################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII Acc65I AccIII AcyI AgeI Alw44I AocI ApaI ApaLI AscI Asp718I AviII BanI BbeI BbrPI BbvCI BglI BsaHI BseAI BsgI BsiEI BsiWI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII BssSI Bsu36I Cfr10I ClaI CpoI DraII DrdI EagI EclXI Eco52I Eco81I EcoO109I EcoRV FseI FspI Hin1I KasI KpnI KspI MluI MroI NaeI NarI NcoI NdeI NgoMIV NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI Sse8387I SspBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccII 3179 CGCG CG^CG AflII 2204 CTTAAG C^TTAAG AhdI 2786 GACGCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 800 AGCGCT AGC^GCT AspEI 2786 GACGCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2089 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1497 CCCGGG C^[CT]CG[GA]G AvrII 5180 CCTAGG C^CTAGG BalI 2301 TGGCCA TGG^CCA BamHI 2384 GGATCC G^GATCC BbsI 4556 GAAGAC GAAGAC(2/6) BcgI 1213 GCAGTTAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 2204 CTTAAG C^TTAAG BglII 820 AGATCT A^GATCT BlnI 5180 CCTAGG C^CTAGG BlpI 5160 GCTAAGC GC^T[ACGT]AGC Bpu1102I 5160 GCTAAGC GC^T[ACGT]AGC BpuAI 4556 GAAGAC GAAGAC(2/6) BsaAI 1081 TACGTA [CT]AC^GT[GA] BsaWI 2914 TCCGGT [AT]^CCGG[AT] BseRI 4607 GAGGAG GAGGAG(10/8) BsmBI 1798 GAGACG CGTCTC(1/5) BsoBI 1497 CCCGGG C^[CT]CG[GA]G Bst1107I 775 GTATAC GTA^TAC BstEII 4014 GGTTACC G^GT[ACGT]ACC BstPI 4014 GGTTACC G^GT[ACGT]ACC BstUI 3179 CGCG CG^CG BstZ17I 775 GTATAC GTA^TAC CelII 5160 GCTAAGC GC^T[ACGT]AGC DsaI 1589 CCGTGG C^C[GA][CT]GG EaeI 2301 TGGCCA [CT]^GGCC[GA] Eam1105I 2786 GACGCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 800 AGCGCT AGC^GCT EcoNI 1666 CCTCTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 4014 GGTTACC G^GT[ACGT]ACC EcoT14I 5180 CCTAGG C^C[AT][AT]GG FauI 3180 GCGGG CCCGC(4/6) HaeII 800 AGCGCT [GA]GCGC^[CT] HindIII 1235 AAGCTT A^AGCTT MfeI 3472 CAATTG C^AATTG MluNI 2301 TGGCCA TGG^CCA MscI 2301 TGGCCA TGG^CCA MspA1I 4412 CAGCTG C[AC]G^C[GT]G MunI 3472 CAATTG C^AATTG MvnI 3179 CGCG CG^CG NheI 2115 GCTAGC G^CTAGC PflFI 2089 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 4412 CAGCTG CAG^CTG SacI 4799 GAGCTC GAGCT^C SmaI 1497 CCCGGG CCC^GGG SnaBI 1081 TACGTA TAC^GTA SpeI 550 ACTAGT A^CTAGT StuI 5056 AGGCCT AGG^CCT StyI 5180 CCTAGG C^C[AT][AT]GG Tth111I 2089 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 4345 TCTAGA T^CTAGA XcmI 564 CCACCAGAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1497 CCCGGG C^CCGGG XmaI 1497 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 775 GTATAC GT^[AC][GT]AC 1772 GTCTAC AclI 1118 AACGTT AA^CGTT 4471 AACGTT Asp700I 205 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3341 GAATAGATTC BanII 2369 GGGCTC G[GA]GC[CT]^C 4799 GAGCTC BciVI 2511 GGATAC GTATCC(6/5) 429 GTATCC BclI 341 TGATCA T^GATCA 2938 TGATCA BcnI 1497 CCCGG CC^[GC]GG 1498 CCGGG BsaBI 290 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4272 GATAAAAATC BsaI 539 GAGACC GGTCTC(1/5) 2793 GGTCTC BsmI 845 GAATGC GAATGC(1/-1) 5086 GAATGC BsrDI 335 CATTGC GCAATG(2/0) 3437 CATTGC BstBI 1441 TTCGAA TT^CGAA 1945 TTCGAA CfoI 801 GCGC GCG^C 2577 GCGC DraIII 2516 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG 4695 CACAACGTG EcoRI 1948 GAATTC G^AATTC 2910 GAATTC FbaI 341 TGATCA T^GATCA 2938 TGATCA HapII 1498 CCGG C^CGG 2915 CCGG HhaI 801 GCGC GCG^C 2577 GCGC HinP1I 801 GCGC G^CGC 2577 GCGC HpaII 1498 CCGG C^CGG 2915 CCGG MamI 290 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4272 GATAAAAATC MspI 1498 CCGG C^CGG 2915 CCGG NciI 1497 CCCGG CC^[GC]GG 1498 CCGGG NspV 1441 TTCGAA TT^CGAA 1945 TTCGAA PflMI 2091 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3443 CCAGATTGTGG PshAI 3122 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4787 GACGTTAGTC Psp1406I 1118 AACGTT AA^CGTT 4471 AACGTT PstI 869 CTGCAG CTGCA^G 1461 CTGCAG SfuI 1441 TTCGAA TT^CGAA 1945 TTCGAA SspI 4027 AATATT AAT^ATT 4725 AATATT Van91I 2091 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3443 CCAGATTGTGG XmnI 205 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3341 GAATAGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaII BbvI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspLU11I BspMI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII EcoT22I Fnu4HI FokI HaeIII HgaI HincII HindII HinfI HpaI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PshBI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII