##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
Alw44I AocI ApaI ApaLI AscI
Asp718I AviII BanI BbeI BbrPI
BbvCI BglI BsaHI BseAI BsgI
BsiEI BsiWI Bsp1407I BspDI BspEI
BsrBI BsrFI BsrGI BssHII BssSI
Bsu36I Cfr10I ClaI CpoI DraII
DrdI EagI EclXI Eco52I Eco81I
EcoO109I EcoRV FseI FspI Hin1I
KasI KpnI KspI MluI MroI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
Sse8387I SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccII 3179 CGCG CG^CG
AflII 2204 CTTAAG C^TTAAG
AhdI 2786 GACGCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 800 AGCGCT AGC^GCT
AspEI 2786 GACGCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2089 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1497 CCCGGG C^[CT]CG[GA]G
AvrII 5180 CCTAGG C^CTAGG
BalI 2301 TGGCCA TGG^CCA
BamHI 2384 GGATCC G^GATCC
BbsI 4556 GAAGAC GAAGAC(2/6)
BcgI 1213 GCAGTTAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2204 CTTAAG C^TTAAG
BglII 820 AGATCT A^GATCT
BlnI 5180 CCTAGG C^CTAGG
BlpI 5160 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 5160 GCTAAGC GC^T[ACGT]AGC
BpuAI 4556 GAAGAC GAAGAC(2/6)
BsaAI 1081 TACGTA [CT]AC^GT[GA]
BsaWI 2914 TCCGGT [AT]^CCGG[AT]
BseRI 4607 GAGGAG GAGGAG(10/8)
BsmBI 1798 GAGACG CGTCTC(1/5)
BsoBI 1497 CCCGGG C^[CT]CG[GA]G
Bst1107I 775 GTATAC GTA^TAC
BstEII 4014 GGTTACC G^GT[ACGT]ACC
BstPI 4014 GGTTACC G^GT[ACGT]ACC
BstUI 3179 CGCG CG^CG
BstZ17I 775 GTATAC GTA^TAC
CelII 5160 GCTAAGC GC^T[ACGT]AGC
DsaI 1589 CCGTGG C^C[GA][CT]GG
EaeI 2301 TGGCCA [CT]^GGCC[GA]
Eam1105I 2786 GACGCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 800 AGCGCT AGC^GCT
EcoNI 1666 CCTCTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4014 GGTTACC G^GT[ACGT]ACC
EcoT14I 5180 CCTAGG C^C[AT][AT]GG
FauI 3180 GCGGG CCCGC(4/6)
HaeII 800 AGCGCT [GA]GCGC^[CT]
HindIII 1235 AAGCTT A^AGCTT
MfeI 3472 CAATTG C^AATTG
MluNI 2301 TGGCCA TGG^CCA
MscI 2301 TGGCCA TGG^CCA
MspA1I 4412 CAGCTG C[AC]G^C[GT]G
MunI 3472 CAATTG C^AATTG
MvnI 3179 CGCG CG^CG
NheI 2115 GCTAGC G^CTAGC
PflFI 2089 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 4412 CAGCTG CAG^CTG
SacI 4799 GAGCTC GAGCT^C
SmaI 1497 CCCGGG CCC^GGG
SnaBI 1081 TACGTA TAC^GTA
SpeI 550 ACTAGT A^CTAGT
StuI 5056 AGGCCT AGG^CCT
StyI 5180 CCTAGG C^C[AT][AT]GG
Tth111I 2089 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4345 TCTAGA T^CTAGA
XcmI 564 CCACCAGAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1497 CCCGGG C^CCGGG
XmaI 1497 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 775 GTATAC GT^[AC][GT]AC
1772 GTCTAC
AclI 1118 AACGTT AA^CGTT
4471 AACGTT
Asp700I 205 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3341 GAATAGATTC
BanII 2369 GGGCTC G[GA]GC[CT]^C
4799 GAGCTC
BciVI 2511 GGATAC GTATCC(6/5)
429 GTATCC
BclI 341 TGATCA T^GATCA
2938 TGATCA
BcnI 1497 CCCGG CC^[GC]GG
1498 CCGGG
BsaBI 290 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4272 GATAAAAATC
BsaI 539 GAGACC GGTCTC(1/5)
2793 GGTCTC
BsmI 845 GAATGC GAATGC(1/-1)
5086 GAATGC
BsrDI 335 CATTGC GCAATG(2/0)
3437 CATTGC
BstBI 1441 TTCGAA TT^CGAA
1945 TTCGAA
CfoI 801 GCGC GCG^C
2577 GCGC
DraIII 2516 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
4695 CACAACGTG
EcoRI 1948 GAATTC G^AATTC
2910 GAATTC
FbaI 341 TGATCA T^GATCA
2938 TGATCA
HapII 1498 CCGG C^CGG
2915 CCGG
HhaI 801 GCGC GCG^C
2577 GCGC
HinP1I 801 GCGC G^CGC
2577 GCGC
HpaII 1498 CCGG C^CGG
2915 CCGG
MamI 290 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4272 GATAAAAATC
MspI 1498 CCGG C^CGG
2915 CCGG
NciI 1497 CCCGG CC^[GC]GG
1498 CCGGG
NspV 1441 TTCGAA TT^CGAA
1945 TTCGAA
PflMI 2091 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3443 CCAGATTGTGG
PshAI 3122 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4787 GACGTTAGTC
Psp1406I 1118 AACGTT AA^CGTT
4471 AACGTT
PstI 869 CTGCAG CTGCA^G
1461 CTGCAG
SfuI 1441 TTCGAA TT^CGAA
1945 TTCGAA
SspI 4027 AATATT AAT^ATT
4725 AATATT
Van91I 2091 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3443 CCAGATTGTGG
XmnI 205 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3341 GAATAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaII BbvI BfaI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspLU11I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT22I Fnu4HI FokI HaeIII HgaI
HincII HindII HinfI HpaI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NsiI NspI PleI PshBI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII