##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Aor51HI AscI AspEI
AviII BalI BamHI BbrPI BclI
BfrI BglII BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraIII Eam1105I Eco47III EcoO65I EcoRV
EcoT22I FbaI FseI FspI HpaI
MfeI MluI MluNI MroI MscI
MunI NdeI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Alw44I 1656 GTGCAC G^TGCAC
AocI 3297 CCTTAGG CC^T[ACGT]AGG
ApaLI 1656 GTGCAC G^TGCAC
Asp700I 1061 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 478 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 194 GGCGCC GGCGC^C
BbvCI 626 CCTCAGC CCTCAGC(-5/-2)
BcgI 907 CGAACCCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 3204 TACGTG [CT]AC^GT[GA]
BsaBI 920 GATCCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2649 GGTCTC GGTCTC(1/5)
BsmBI 1541 GAGACG CGTCTC(1/5)
BspLU11I 1281 ACATGT A^CATGT
BsrDI 1421 CATTGC GCAATG(2/0)
BssHII 672 GCGCGC G^CGCGC
BstYI 3684 AGATCC [GA]^GATC[CT]
Bsu36I 3297 CCTTAGG CC^T[ACGT]AGG
DrdI 1994 GACAGCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3297 CCTTAGG CC^T[ACGT]AGG
EcoRI 3927 GAATTC G^AATTC
KasI 194 GGCGCC G^GCGCC
MamI 920 GATCCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 3684 AGATCC [GA]^GATC[CT]
NaeI 108 GCCGGC GCC^GGC
NarI 194 GGCGCC GG^CGCC
NgoMIV 108 GCCGGC G^CCGGC
NheI 2050 GCTAGC G^CTAGC
NotI 1 GCGGCCGC GC^GGCCGC
PflFI 478 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1357 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 3383 AGTACT AGT^ACT
SexAI 1388 ACCAGGT A^CC[AT]GGT
SfoI 194 GGCGCC GGC^GCC
SspI 3103 AATATT AAT^ATT
TaqI 526 TCGA T^CGA
Tth111I 478 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 526 TCGA T^CGA
Van91I 1357 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 3684 AGATCC [GA]^GATC[CT]
XmnI 1061 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 2196 GGTACC G^GTACC
2437 GGTACC
AlwNI 1238 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1749 CAGGCCCTG
ApaI 745 GGGCCC GGGCC^C
2719 GGGCCC
AseI 3374 ATTAAT AT^TAAT
3975 ATTAAT
AsnI 3374 ATTAAT AT^TAAT
3975 ATTAAT
Asp718I 2196 GGTACC G^GTACC
2437 GGTACC
BciVI 3672 GGATAC GTATCC(6/5)
2604 GTATCC
BglI 20 GCCCCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1474 GCCTTGGGGGC
BlpI 33 GCTCAGC GC^T[ACGT]AGC
773 GCTGAGC
Bpu1102I 33 GCTCAGC GC^T[ACGT]AGC
773 GCTGAGC
BspMI 340 ACCTGC ACCTGC(4/8)
3034 ACCTGC
CelII 33 GCTCAGC GC^T[ACGT]AGC
773 GCTGAGC
HincII 2730 GTTGAC GT[CT]^[GA]AC
3860 GTCAAC
HindII 2730 GTTGAC GT[CT]^[GA]AC
3860 GTCAAC
HindIII 1228 AAGCTT A^AGCTT
2503 AAGCTT
KpnI 2196 GGTACC GGTAC^C
2437 GGTACC
PshBI 3374 ATTAAT AT^TAAT
3975 ATTAAT
PvuII 2542 CAGCTG CAG^CTG
2884 CAGCTG
SacI 31 GAGCTC GAGCT^C
3664 GAGCTC
SmaI 372 CCCGGG CCC^GGG
579 CCCGGG
SmlI 3218 CTTGAG C^T[CT][GA]AG
3869 CTCAAG
SphI 3223 GCATGC GCATG^C
3903 GCATGC
XbaI 3016 TCTAGA T^CTAGA
3425 TCTAGA
XmaCI 372 CCCGGG C^CCGGG
579 CCCGGG
XmaI 372 CCCGGG C^CCGGG
579 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AspHI
AvaI AvaII AvrII BanI BanII
BbsI BbvI BcnI BfaI BlnI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI