##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AccII AccIII AclI AgeI AhdI
Aor51HI ApaI AscI AspEI AspI
AvaI BalI BamHI BbeI BbrPI
BbvCI BclI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspEI BspHI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstXI BstZ17I CelII CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoT22I FauI FbaI FseI HaeII
HpaI KasI KspI MamI MluI
MluNI MroI MscI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 3200 GACGTC GACGT^C
Acc65I 1477 GGTACC G^GTACC
AcyI 3200 GACGTC G[GA]^CG[CT]C
AocI 1824 CCTTAGG CC^T[ACGT]AGG
Asp718I 1477 GGTACC G^GTACC
AvaII 2500 GGACC G^G[AT]CC
AviII 1906 TGCGCA TGC^GCA
AvrII 1673 CCTAGG C^CTAGG
BcgI 2311 GCACAAAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1829 GGATAC GTATCC(6/5)
BcnI 147 CCGGG CC^[GC]GG
BglI 3416 GCCAAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1673 CCTAGG C^CTAGG
BsaHI 3200 GACGTC G[GA]^CG[CT]C
BsgI 4169 CTGCAC GTGCAG(16/14)
Bsp1407I 5045 TGTACA T^GTACA
BspDI 1651 ATCGAT AT^CGAT
BspMI 2851 ACCTGC ACCTGC(4/8)
BsrBI 4709 CCGCTC CCGCTC(-3/-3)
BsrDI 3676 CATTGC GCAATG(2/0)
BsrFI 4173 ACCGGC [GA]^CCGG[CT]
BsrGI 5045 TGTACA T^GTACA
BstBI 2477 TTCGAA TT^CGAA
Bsu36I 1824 CCTTAGG CC^T[ACGT]AGG
CfoI 1907 GCGC GCG^C
Cfr10I 4173 ACCGGC [GA]^CCGG[CT]
Cfr13I 2500 GGACC G^G[ACGT]CC
ClaI 1651 ATCGAT AT^CGAT
DsaI 2988 CCATGG C^C[GA][CT]GG
EaeI 1143 TGGCCG [CT]^GGCC[GA]
Eco81I 1824 CCTTAGG CC^T[ACGT]AGG
EcoNI 3100 CCTAGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1906 TGCGCA TGC^GCA
HgaI 1544 GCGTC GACGC(5/10)
HhaI 1907 GCGC GCG^C
Hin1I 3200 GACGTC G[GA]^CG[CT]C
HinP1I 1907 GCGC G^CGC
HincII 637 GTTGAC GT[CT]^[GA]AC
HindII 637 GTTGAC GT[CT]^[GA]AC
KpnI 1477 GGTACC GGTAC^C
MfeI 4975 CAATTG C^AATTG
MunI 4975 CAATTG C^AATTG
NciI 147 CCGGG CC^[GC]GG
NcoI 2988 CCATGG C^CATGG
NspV 2477 TTCGAA TT^CGAA
PflMI 922 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I 2500 GGACC G^G[ACGT]CC
ScaI 317 AGTACT AGT^ACT
SfuI 2477 TTCGAA TT^CGAA
SpeI 343 ACTAGT A^CTAGT
SphI 726 GCATGC GCATG^C
SspBI 5045 TGTACA T^GTACA
StuI 4268 AGGCCT AGG^CCT
Van91I 922 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 4970 CCAGTCAATTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AccI 1759 GTCTAC GT^[AC][GT]AC
2471 GTAGAC
AflII 290 CTTAAG C^TTAAG
313 CTTAAG
Alw44I 630 GTGCAC G^TGCAC
1500 GTGCAC
ApaLI 630 GTGCAC G^TGCAC
1500 GTGCAC
BanI 816 GGTGCC G^G[CT][GA]CC
1477 GGTACC
BfrI 290 CTTAAG C^TTAAG
313 CTTAAG
BsmFI 2499 GGGAC GGGAC(10/14)
4735 GTCCC
BsmI 3187 GAATGC GAATGC(1/-1)
4216 GAATGC
BstAPI 2765 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3671 GCACACATTGC
HapII 147 CCGG C^CGG
4174 CCGG
HindIII 513 AAGCTT A^AGCTT
903 AAGCTT
HpaII 147 CCGG C^CGG
4174 CCGG
MspI 147 CCGG C^CGG
4174 CCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI BanII BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspLU11I BsrI BssKI
BstF5I BstNI BstYI Cac8I DdeI
DpnII DraI EarI EcoRI EcoRII
EcoRV EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PshBI PstI PvuII RsaI
SacI Sau3AI ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI