##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AclI AcsI AflIII Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspI AvrII BamHI BciVI
BclI BlnI BsiWI BspDI BspHI
BspLU11I BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI DraI DrdI
Eco47III EcoO65I EcoRI FbaI HindIII
HpaI MfeI MluI MunI NdeI
NheI NotI NruI NspV PacI
PflFI PmeI PshAI PshBI Psp1406I
PvuI RcaI SalI SbfI SexAI
SfuI SpeI Sse8387I SwaI Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 3821 GACGTC GACGT^C
Acc65I 1569 GGTACC G^GTACC
AflII 2390 CTTAAG C^TTAAG
AhdI 2543 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 3483 GGATC GGATC(4/5)
Asp718I 1569 GGTACC G^GTACC
AspEI 2543 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1644 GGCGCC GGCGC^C
BbrPI 3425 CACGTG CAC^GTG
BcgI 2160 GCATGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2390 CTTAAG C^TTAAG
BglII 3123 AGATCT A^GATCT
BsaBI 1357 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2264 GAGACG CGTCTC(1/5)
BsmI 899 GAATGC GAATGC(1/-1)
Bsp1407I 4924 TGTACA T^GTACA
BsrGI 4924 TGTACA T^GTACA
BssHII 75 GCGCGC G^CGCGC
BstYI 3123 AGATCT [GA]^GATC[CT]
CpoI 1149 CGGTCCG CG^G[AT]CCG
Eam1105I 2543 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 845 GATATC GAT^ATC
EcoT22I 901 ATGCAT ATGCA^T
HincII 3902 GTCAAC GT[CT]^[GA]AC
HindII 3902 GTCAAC GT[CT]^[GA]AC
KasI 1644 GGCGCC G^GCGCC
KpnI 1569 GGTACC GGTAC^C
MamI 1357 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 3123 AGATCT [GA]^GATC[CT]
NarI 1644 GGCGCC GG^CGCC
NsiI 901 ATGCAT ATGCA^T
PaeR7I 4525 CTCGAG C^TCGAG
PmaCI 3425 CACGTG CAC^GTG
PmlI 3425 CACGTG CAC^GTG
RsrII 1149 CGGTCCG CG^G[AT]CCG
ScaI 2147 AGTACT AGT^ACT
SfiI 3307 GGCCCACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1644 GGCGCC GGC^GCC
SgrAI 4182 CACCGGTG C[GA]^CCGG[CT]G
SnaBI 635 TACGTA TAC^GTA
SspBI 4924 TGTACA T^GTACA
SspI 4604 AATATT AAT^ATT
XhoI 4525 CTCGAG C^TCGAG
XhoII 3123 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccIII 3146 TCCGGA T^CCGGA
3480 TCCGGA
AgeI 2769 ACCGGT A^CCGGT
4183 ACCGGT
AocI 2207 CCTGAGG CC^T[ACGT]AGG
4436 CCTCAGG
AviII 648 TGCGCA TGC^GCA
1254 TGCGCA
BbsI 2773 GTCTTC GAAGAC(2/6)
3142 GTCTTC
BfaI 1493 CTAG C^TAG
2825 CTAG
BpuAI 2773 GTCTTC GAAGAC(2/6)
3142 GTCTTC
BseAI 3146 TCCGGA T^CCGGA
3480 TCCGGA
BspEI 3146 TCCGGA T^CCGGA
3480 TCCGGA
Bsu36I 2207 CCTGAGG CC^T[ACGT]AGG
4436 CCTCAGG
DraIII 233 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
3914 CACTCGGTG
Eco81I 2207 CCTGAGG CC^T[ACGT]AGG
4436 CCTCAGG
FseI 3767 GGCCGGCC GGCCGG^CC
3881 GGCCGGCC
FspI 648 TGCGCA TGC^GCA
1254 TGCGCA
MaeI 1493 CTAG C^TAG
2825 CTAG
MroI 3146 TCCGGA T^CCGGA
3480 TCCGGA
NcoI 417 CCATGG C^CATGG
4667 CCATGG
PinAI 2769 ACCGGT A^CCGGT
4183 ACCGGT
SacI 3038 GAGCTC GAGCT^C
3830 GAGCTC
SapI 1859 GAAGAGC GCTCTTC(1/4)
3556 GAAGAGC
SphI 903 GCATGC GCATG^C
1717 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BglI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI