##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AccI AclI AcsI AflII AgeI
AhdI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII BciVI
BfrI BsaBI BsiWI BspDI BsrDI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI Eam1105I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI MamI MfeI
MluI MseI MunI NcoI NdeI
NotI NsiI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacI SalI SbfI ScaI
SfuI SnaBI SpeI SphI Sse8387I
SspI SwaI Tru9I Tth111I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 327 GACGTC GACGT^C
Acc65I 2041 GGTACC G^GTACC
Aor51HI 897 AGCGCT AGC^GCT
Asp718I 2041 GGTACC G^GTACC
AvrII 2880 CCTAGG C^CTAGG
BamHI 373 GGATCC G^GATCC
BbvCI 2795 GCTGAGG CCTCAGC(-5/-2)
BclI 2761 TGATCA T^GATCA
BlnI 2880 CCTAGG C^CTAGG
BlpI 2318 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2318 GCTGAGC GC^T[ACGT]AGC
BsmBI 1553 CGTCTC CGTCTC(1/5)
BsmI 4567 GAATGC GAATGC(1/-1)
BspHI 347 TCATGA T^CATGA
BspLU11I 3070 ACATGT A^CATGT
CelII 2318 GCTGAGC GC^T[ACGT]AGC
Eco47III 897 AGCGCT AGC^GCT
EcoNI 4734 CCTCGGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2761 TGATCA T^GATCA
HincII 4643 GTCAAC GT[CT]^[GA]AC
HindII 4643 GTCAAC GT[CT]^[GA]AC
KpnI 2041 GGTACC GGTAC^C
NheI 3824 GCTAGC G^CTAGC
NruI 4432 TCGCGA TCG^CGA
PaeR7I 2912 CTCGAG C^TCGAG
RcaI 347 TCATGA T^CATGA
SapI 1316 GAAGAGC GCTCTTC(1/4)
SgrAI 490 CACCGGCG C[GA]^CCGG[CT]G
XhoI 2912 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BbeI 885 GGCGCC GGCGC^C
2022 GGCGCC
BcgI 2046 CGACTGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2299 GCATTGGCATCG
BglII 961 AGATCT A^GATCT
1258 AGATCT
BsiEI 70 CGGCCG CG[GA][CT]^CG
1361 CGGCCG
Bsp1407I 1723 TGTACA T^GTACA
2626 TGTACA
BsrGI 1723 TGTACA T^GTACA
2626 TGTACA
BstAPI 553 GCAGTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3549 GCAGGCAGTGC
DraIII 2973 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
3283 CACTTTGTG
EagI 70 CGGCCG C^GGCCG
1361 CGGCCG
EclXI 70 CGGCCG C^GGCCG
1361 CGGCCG
Eco52I 70 CGGCCG C^GGCCG
1361 CGGCCG
KasI 885 GGCGCC G^GCGCC
2022 GGCGCC
NarI 885 GGCGCC GG^CGCC
2022 GGCGCC
SexAI 2562 ACCAGGT A^CC[AT]GGT
3568 ACCTGGT
SfiI 43 GGCCGCTCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1854 GGCCTCAAGGGCC
SfoI 885 GGCGCC GGC^GCC
2022 GGCGCC
SspBI 1723 TGTACA T^GTACA
2626 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrI BssHII BssKI
BssSI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PshAI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI