##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
Acc65I AccII AgeI AhdI AocI
Aor51HI AscI Asp718I AspEI AviII
AvrII BamHI BanI BbeI BbrPI
BcgI BcnI BglI BlnI BlpI
Bpu1102I BsiEI BsiWI BspDI BspHI
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I FseI
FspI HaeII HhaI HinP1I KasI
KpnI KspI MluI MspA1I MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NspV PinAI
PmaCI PmeI PmlI PshAI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI Sse8387I
SspI StuI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 2196 GACGTC GACGT^C
AclI 2734 AACGTT AA^CGTT
AcyI 2196 GACGTC G[GA]^CG[CT]C
AflII 74 CTTAAG C^TTAAG
Alw44I 3274 GTGCAC G^TGCAC
ApaI 3844 GGGCCC GGGCC^C
ApaLI 3274 GTGCAC G^TGCAC
AseI 4371 ATTAAT AT^TAAT
AsnI 4371 ATTAAT AT^TAAT
AspI 2515 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 472 CTCGAG C^[CT]CG[GA]G
BbvCI 3532 CCTCAGC CCTCAGC(-5/-2)
BciVI 2023 GTATCC GTATCC(6/5)
BfrI 74 CTTAAG C^TTAAG
BsaAI 4270 TACGTA [CT]AC^GT[GA]
BsaHI 2196 GACGTC G[GA]^CG[CT]C
BsmBI 3395 GAGACG CGTCTC(1/5)
BsoBI 472 CTCGAG C^[CT]CG[GA]G
BspMI 1460 ACCTGC ACCTGC(4/8)
BsrDI 442 GCAATG GCAATG(2/0)
BssSI 3576 CTCGTG CACGAG(-5/-1)
DsaI 3569 CCACGG C^C[GA][CT]GG
EcoNI 4897 CCTAAAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 2453 GCGGG CCCGC(4/6)
HgaI 3153 GACGC GACGC(5/10)
Hin1I 2196 GACGTC G[GA]^CG[CT]C
HpaI 1263 GTTAAC GTT^AAC
PacI 4368 TTAATTAA TTAAT^TAA
PaeR7I 472 CTCGAG C^TCGAG
PflFI 2515 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 4371 ATTAAT AT^TAAT
Psp1406I 2734 AACGTT AA^CGTT
SapI 771 GCTCTTC GCTCTTC(1/4)
SnaBI 4270 TACGTA TAC^GTA
SpeI 4857 ACTAGT A^CTAGT
SphI 970 GCATGC GCATG^C
SwaI 1429 ATTTAAAT ATTT^AAAT
Tth111I 2515 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 682 CCACTAGAAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 472 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 2077 GTCTAC GT^[AC][GT]AC
4930 GTAGAC
AccIII 98 TCCGGA T^CCGGA
1216 TCCGGA
AciI 1048 GCGG CCGC(-3/-1)
2453 GCGG
BalI 2340 TGGCCA TGG^CCA
4331 TGGCCA
BanII 63 GGGCTC G[GA]GC[CT]^C
3844 GGGCCC
BclI 2681 TGATCA T^GATCA
3262 TGATCA
BsaBI 3558 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3675 GATGTGAATC
BsaI 3695 GAGACC GGTCTC(1/5)
3573 GGTCTC
BsaWI 98 TCCGGA [AT]^CCGG[AT]
1216 TCCGGA
BseAI 98 TCCGGA T^CCGGA
1216 TCCGGA
Bsp1407I 2251 TGTACA T^GTACA
4404 TGTACA
BspEI 98 TCCGGA T^CCGGA
1216 TCCGGA
BsrGI 2251 TGTACA T^GTACA
4404 TGTACA
BstAPI 936 GCAGAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1280 GCAGCATCTGC
BstXI 2491 CCAAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3308 CCAGATTAGTGG
EaeI 2340 TGGCCA [CT]^GGCC[GA]
4331 TGGCCA
FbaI 2681 TGATCA T^GATCA
3262 TGATCA
HapII 99 CCGG C^CGG
1217 CCGG
HindIII 660 AAGCTT A^AGCTT
2134 AAGCTT
HpaII 99 CCGG C^CGG
1217 CCGG
MamI 3558 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3675 GATGTGAATC
MluNI 2340 TGGCCA TGG^CCA
4331 TGGCCA
MroI 98 TCCGGA T^CCGGA
1216 TCCGGA
MscI 2340 TGGCCA TGG^CCA
4331 TGGCCA
MspI 99 CCGG C^CGG
1217 CCGG
PpuMI 1550 AGGTCCT [GA]G^G[AT]CC[CT]
2637 GGGTCCC
SspBI 2251 TGTACA T^GTACA
4404 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BbsI BbvI BfaI BglII BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspLU11I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRI EcoRII EcoRV EcoT14I
EcoT22I Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MunI MvaI MwoI NdeI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XhoII XmnI