################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AhdI ApoI AscI AseI AsnI Asp718I AspEI AviII BbrPI BciVI BfrI BlpI Bpu1102I BsaBI BseAI BsiWI BspEI BsrDI Bst1107I BstBI BstZ17I CelII CpoI DraI Eam1105I EcoRI EcoRV EcoT22I FspI HpaI KpnI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PmaCI PmeI PmlI PshAI PshBI Psp1406I RsrII SalI SapI SbfI SfuI SnaBI SpeI Sse8387I SspI SwaI Tsp509I XbaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AflIII 1621 ACATGT A^C[GA][CT]GT AgeI 770 ACCGGT A^CCGGT Alw44I 2927 GTGCAC G^TGCAC AocI 1990 CCTCAGG CC^T[ACGT]AGG Aor51HI 1231 AGCGCT AGC^GCT ApaLI 2927 GTGCAC G^TGCAC Asp700I 1334 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 2264 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 20 CCTAGG C^CTAGG BclI 3541 TGATCA T^GATCA BlnI 20 CCTAGG C^CTAGG BsaAI 4443 TACGTG [CT]AC^GT[GA] BspDI 1458 ATCGAT AT^CGAT BspLU11I 1621 ACATGT A^CATGT BssSI 1440 CACGAG CACGAG(-5/-1) BstXI 3829 CCAAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1990 CCTCAGG CC^T[ACGT]AGG ClaI 1458 ATCGAT AT^CGAT DraIII 1440 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1605 GACCGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 2303 GAAGAG CTCTTC(1/4) Eco47III 1231 AGCGCT AGC^GCT Eco81I 1990 CCTCAGG CC^T[ACGT]AGG FbaI 3541 TGATCA T^GATCA FseI 2950 GGCCGGCC GGCCGG^CC HincII 2556 GTCAAC GT[CT]^[GA]AC HindII 2556 GTCAAC GT[CT]^[GA]AC HindIII 287 AAGCTT A^AGCTT Ksp632I 2303 GAAGAG CTCTTC(1/4) PaeR7I 3708 CTCGAG C^TCGAG PflFI 2264 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 770 ACCGGT A^CCGGT PvuI 86 CGATCG CGAT^CG ScaI 1324 AGTACT AGT^ACT SexAI 3593 ACCTGGT A^CC[AT]GGT SfiI 2975 GGCCGCTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Tth111I 2264 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 3708 CTCGAG C^TCGAG XmnI 1334 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AatII 438 GACGTC GACGT^C 4062 GACGTC BalI 703 TGGCCA TGG^CCA 4600 TGGCCA BamHI 339 GGATCC G^GATCC 4429 GGATCC BfaI 21 CTAG C^TAG 660 CTAG BglII 898 AGATCT A^GATCT 2323 AGATCT BsmBI 1781 GAGACG CGTCTC(1/5) 4263 GAGACG BspHI 1130 TCATGA T^CATGA 1615 TCATGA BssHII 72 GCGCGC G^CGCGC 127 GCGCGC BstAPI 1859 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3338 GCAAAACCTGC BstEII 3263 GGTCACC G^GT[ACGT]ACC 3597 GGTCACC BstPI 3263 GGTCACC G^GT[ACGT]ACC 3597 GGTCACC EcoO65I 3263 GGTCACC G^GT[ACGT]ACC 3597 GGTCACC MaeI 21 CTAG C^TAG 660 CTAG MluNI 703 TGGCCA TGG^CCA 4600 TGGCCA MscI 703 TGGCCA TGG^CCA 4600 TGGCCA MseI 262 TTAA T^TAA 279 TTAA PflMI 851 CCAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1241 CCAGCTCATGG RcaI 1130 TCATGA T^CATGA 1615 TCATGA SacI 573 GAGCTC GAGCT^C 3053 GAGCTC SgrAI 11 CGCCGGCG C[GA]^CCGG[CT]G 2389 CGCCGGCG TfiI 1061 GATTC G^A[AT]TC 1193 GAATC Tru9I 262 TTAA T^TAA 279 TTAA Van91I 851 CCAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1241 CCAGCTCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbeI BbsI BbvCI BbvI BcgI BcnI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI SphI SspBI StuI StyI TaqI TseI Tsp45I TspRI TthHB8I XcmI XhoII XmaCI XmaI