##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AhdI ApoI AscI AseI
AsnI Asp718I AspEI AviII BbrPI
BciVI BfrI BlpI Bpu1102I BsaBI
BseAI BsiWI BspEI BsrDI Bst1107I
BstBI BstZ17I CelII CpoI DraI
Eam1105I EcoRI EcoRV EcoT22I FspI
HpaI KpnI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RsrII SalI SapI SbfI SfuI
SnaBI SpeI Sse8387I SspI SwaI
Tsp509I XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflIII 1621 ACATGT A^C[GA][CT]GT
AgeI 770 ACCGGT A^CCGGT
Alw44I 2927 GTGCAC G^TGCAC
AocI 1990 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1231 AGCGCT AGC^GCT
ApaLI 2927 GTGCAC G^TGCAC
Asp700I 1334 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2264 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 20 CCTAGG C^CTAGG
BclI 3541 TGATCA T^GATCA
BlnI 20 CCTAGG C^CTAGG
BsaAI 4443 TACGTG [CT]AC^GT[GA]
BspDI 1458 ATCGAT AT^CGAT
BspLU11I 1621 ACATGT A^CATGT
BssSI 1440 CACGAG CACGAG(-5/-1)
BstXI 3829 CCAAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1990 CCTCAGG CC^T[ACGT]AGG
ClaI 1458 ATCGAT AT^CGAT
DraIII 1440 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1605 GACCGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2303 GAAGAG CTCTTC(1/4)
Eco47III 1231 AGCGCT AGC^GCT
Eco81I 1990 CCTCAGG CC^T[ACGT]AGG
FbaI 3541 TGATCA T^GATCA
FseI 2950 GGCCGGCC GGCCGG^CC
HincII 2556 GTCAAC GT[CT]^[GA]AC
HindII 2556 GTCAAC GT[CT]^[GA]AC
HindIII 287 AAGCTT A^AGCTT
Ksp632I 2303 GAAGAG CTCTTC(1/4)
PaeR7I 3708 CTCGAG C^TCGAG
PflFI 2264 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 770 ACCGGT A^CCGGT
PvuI 86 CGATCG CGAT^CG
ScaI 1324 AGTACT AGT^ACT
SexAI 3593 ACCTGGT A^CC[AT]GGT
SfiI 2975 GGCCGCTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tth111I 2264 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3708 CTCGAG C^TCGAG
XmnI 1334 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 438 GACGTC GACGT^C
4062 GACGTC
BalI 703 TGGCCA TGG^CCA
4600 TGGCCA
BamHI 339 GGATCC G^GATCC
4429 GGATCC
BfaI 21 CTAG C^TAG
660 CTAG
BglII 898 AGATCT A^GATCT
2323 AGATCT
BsmBI 1781 GAGACG CGTCTC(1/5)
4263 GAGACG
BspHI 1130 TCATGA T^CATGA
1615 TCATGA
BssHII 72 GCGCGC G^CGCGC
127 GCGCGC
BstAPI 1859 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3338 GCAAAACCTGC
BstEII 3263 GGTCACC G^GT[ACGT]ACC
3597 GGTCACC
BstPI 3263 GGTCACC G^GT[ACGT]ACC
3597 GGTCACC
EcoO65I 3263 GGTCACC G^GT[ACGT]ACC
3597 GGTCACC
MaeI 21 CTAG C^TAG
660 CTAG
MluNI 703 TGGCCA TGG^CCA
4600 TGGCCA
MscI 703 TGGCCA TGG^CCA
4600 TGGCCA
MseI 262 TTAA T^TAA
279 TTAA
PflMI 851 CCAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1241 CCAGCTCATGG
RcaI 1130 TCATGA T^CATGA
1615 TCATGA
SacI 573 GAGCTC GAGCT^C
3053 GAGCTC
SgrAI 11 CGCCGGCG C[GA]^CCGG[CT]G
2389 CGCCGGCG
TfiI 1061 GATTC G^A[AT]TC
1193 GAATC
Tru9I 262 TTAA T^TAA
279 TTAA
Van91I 851 CCAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1241 CCAGCTCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbsI
BbvCI BbvI BcgI BcnI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
KspI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI SphI SspBI StuI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI XhoII XmaCI XmaI