##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI Alw44I ApaLI AscI Asp718I
AspEI AspI AviII AvrII BalI
BbvCI BcgI BclI BfrI BglII
BlnI BlpI Bpu1102I BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BsrGI
BssSI Bst1107I BstEII BstPI BstZ17I
CelII ClaI DrdI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KpnI
MluI MluNI MroI MscI NheI
NotI NruI NsiI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PvuI SacI SalI SbfI ScaI
SexAI SfiI SgrAI SnaBI SpeI
SphI Sse8387I SspBI StuI Tth111I
Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AclI 1946 AACGTT AA^CGTT
AlwNI 558 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3482 CCTAAGG CC^T[ACGT]AGG
Aor51HI 2618 AGCGCT AGC^GCT
AspHI 374 GTGCTC G[AT]GC[AT]^C
BamHI 2346 GGATCC G^GATCC
BbrPI 1877 CACGTG CAC^GTG
BsaBI 3095 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4470 ACCGGA [AT]^CCGG[AT]
BsgI 1073 GTGCAG GTGCAG(16/14)
BsiEI 576 CGGCCG CG[GA][CT]^CG
BsiHKAI 374 GTGCTC G[AT]GC[AT]^C
BspLU11I 36 ACATGT A^CATGT
BsrFI 2 GCCGGC [GA]^CCGG[CT]
BstAPI 557 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2430 TTCGAA TT^CGAA
Bsu36I 3482 CCTAAGG CC^T[ACGT]AGG
Cfr10I 2 GCCGGC [GA]^CCGG[CT]
CpoI 544 CGGTCCG CG^G[AT]CCG
DraII 196 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 1234 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 576 CGGCCG C^GGCCG
EclXI 576 CGGCCG C^GGCCG
Eco47III 2618 AGCGCT AGC^GCT
Eco52I 576 CGGCCG C^GGCCG
Eco81I 3482 CCTAAGG CC^T[ACGT]AGG
EcoO109I 196 GGGGCCC [GA]G^G[ACGT]CC[CT]
HgaI 3936 GCGTC GACGC(5/10)
HindIII 4757 AAGCTT A^AGCTT
KspI 644 CCGCGG CCGC^GG
MamI 3095 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2 GCCGGC GCC^GGC
NcoI 2688 CCATGG C^CATGG
NdeI 2208 CATATG CA^TATG
NgoMIV 2 GCCGGC G^CCGGC
NspV 2430 TTCGAA TT^CGAA
PacI 2030 TTAATTAA TTAAT^TAA
PmaCI 1877 CACGTG CAC^GTG
PmlI 1877 CACGTG CAC^GTG
Psp1406I 1946 AACGTT AA^CGTT
RsrII 544 CGGTCCG CG^G[AT]CCG
SacII 644 CCGCGG CCGC^GG
SfuI 2430 TTCGAA TT^CGAA
SspI 4615 AATATT AAT^ATT
SwaI 3054 ATTTAAAT ATTT^AAAT
Tsp45I 1089 GTCAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 36 ACATGT A^C[GA][CT]GT
2733 ACGTGT
ApaI 197 GGGCCC GGGCC^C
449 GGGCCC
BglI 683 GCCTGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1518 GCCAGCTAGGC
BsaAI 1877 CACGTG [CT]AC^GT[GA]
2732 TACGTG
BseRI 695 GAGGAG GAGGAG(10/8)
1700 GAGGAG
BsrBI 639 CCGCTC CCGCTC(-3/-3)
221 GAGCGG
BsrDI 1317 CATTGC GCAATG(2/0)
1784 GCAATG
BssHII 190 GCGCGC G^CGCGC
226 GCGCGC
EcoNI 2593 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3346 CCTGTAAGAGG
EcoT14I 81 CCAAGG C^C[AT][AT]GG
2688 CCATGG
MfeI 2226 CAATTG C^AATTG
2779 CAATTG
MunI 2226 CAATTG C^AATTG
2779 CAATTG
SapI 1850 GAAGAGC GCTCTTC(1/4)
2605 GAAGAGC
SmaI 67 CCCGGG CCC^GGG
452 CCCGGG
StyI 81 CCAAGG C^C[AT][AT]GG
2688 CCATGG
TaqI 2431 TCGA T^CGA
2473 TCGA
TthHB8I 2431 TCGA T^CGA
2473 TCGA
XbaI 3419 TCTAGA T^CTAGA
4397 TCTAGA
XmaCI 67 CCCGGG C^CCGGG
452 CCCGGG
XmaI 67 CCCGGG C^CCGGG
452 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI ApoI AseI
AsnI Asp700I AvaI AvaII BanI
BanII BbeI BbsI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PleI
PshBI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI TfiI Tru9I TseI
Tsp509I TspRI XhoII XmnI