##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccIII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
AspEI AspI AviII AvrII BamHI
BbrPI BbvCI BciVI BclI BglII
BlnI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BssHII
BstBI BstEII BstPI Bsu36I ClaI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I FbaI FseI
FspI HgaI HindIII HpaI KspI
MfeI MroI MunI NaeI NcoI
NgoMIV NotI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI PvuI RcaI SacI SacII
SalI ScaI SfiI SfuI SphI
StuI TaqI Tth111I TthHB8I XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 1306 GGTACC G^GTACC
AclI 3299 AACGTT AA^CGTT
AcyI 96 GGCGCC G[GA]^CG[CT]C
AflII 3187 CTTAAG C^TTAAG
Alw44I 2763 GTGCAC G^TGCAC
ApaLI 2763 GTGCAC G^TGCAC
Asp718I 1306 GGTACC G^GTACC
AvaI 52 CCCGGG C^[CT]CG[GA]G
BanII 2097 GAGCCC G[GA]GC[CT]^C
BbeI 96 GGCGCC GGCGC^C
BcgI 720 CGACTGTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3187 CTTAAG C^TTAAG
BlpI 75 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 75 GCTCAGC GC^T[ACGT]AGC
BsaBI 1355 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 96 GGCGCC G[GA]^CG[CT]C
BsaI 282 GAGACC GGTCTC(1/5)
BsoBI 52 CCCGGG C^[CT]CG[GA]G
BspLU11I 4482 ACATGT A^CATGT
BsrBI 217 GAGCGG CCGCTC(-3/-3)
BssSI 4643 CTCGTG CACGAG(-5/-1)
Bst1107I 3265 GTATAC GTA^TAC
BstAPI 1879 GCACACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2497 CCACATACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 4536 AGATCC [GA]^GATC[CT]
BstZ17I 3265 GTATAC GTA^TAC
CelII 75 GCTCAGC GC^T[ACGT]AGC
CpoI 301 CGGTCCG CG^G[AT]CCG
DraIII 4716 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 836 CCGTGG C^C[GA][CT]GG
EcoNI 758 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 96 GGCGCC [GA]GCGC^[CT]
Hin1I 96 GGCGCC G[GA]^CG[CT]C
KasI 96 GGCGCC G^GCGCC
KpnI 1306 GGTACC GGTAC^C
MamI 1355 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 4536 AGATCC [GA]^GATC[CT]
MluI 236 ACGCGT A^CGCGT
NarI 96 GGCGCC GG^CGCC
NdeI 4636 CATATG CA^TATG
NruI 717 TCGCGA TCG^CGA
PpuMI 2008 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 3299 AACGTT AA^CGTT
PstI 1886 CTGCAG CTGCA^G
RsrII 301 CGGTCCG CG^G[AT]CCG
SapI 1469 GAAGAGC GCTCTTC(1/4)
SbfI 1885 CCTGCAGG CCTGCA^GG
SexAI 2978 ACCAGGT A^CC[AT]GGT
SfcI 1886 CTGCAG C^T[GA][CT]AG
SfoI 96 GGCGCC GGC^GCC
SgrAI 1813 CACCGGCG C[GA]^CCGG[CT]G
SmaI 52 CCCGGG CCC^GGG
SnaBI 1511 TACGTA TAC^GTA
Sse8387I 1885 CCTGCAGG CCTGCA^GG
XhoII 4536 AGATCC [GA]^GATC[CT]
XmaCI 52 CCCGGG C^CCGGG
XmaI 52 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 236 ACGCGT A^C[GA][CT]GT
4482 ACATGT
AlwNI 1136 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1981 CAGTATCTG
Asp700I 1943 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3231 GAACAAGTTC
BalI 1423 TGGCCA TGG^CCA
1848 TGGCCA
BglI 1910 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3984 GCCTTTTAGGC
BsaAI 1205 TACGTG [CT]AC^GT[GA]
1511 TACGTA
BsmAI 282 GAGAC GTCTC(1/5)
1247 GAGAC
Bsp1407I 2621 TGTACA T^GTACA
2882 TGTACA
BsrDI 828 CATTGC GCAATG(2/0)
735 GCAATG
BsrFI 251 GCCGGT [GA]^CCGG[CT]
1814 ACCGGC
BsrGI 2621 TGTACA T^GTACA
2882 TGTACA
Cfr10I 251 GCCGGT [GA]^CCGG[CT]
1814 ACCGGC
EcoRV 1490 GATATC GAT^ATC
3840 GATATC
EcoT14I 1176 CCTTGG C^C[AT][AT]GG
3496 CCAAGG
HincII 2808 GTCAAC GT[CT]^[GA]AC
4199 GTTGAC
HindII 2808 GTCAAC GT[CT]^[GA]AC
4199 GTTGAC
MluNI 1423 TGGCCA TGG^CCA
1848 TGGCCA
MscI 1423 TGGCCA TGG^CCA
1848 TGGCCA
NheI 339 GCTAGC G^CTAGC
472 GCTAGC
NspI 1842 ACATGC [GA]CATG^[CT]
4482 ACATGT
SpeI 2412 ACTAGT A^CTAGT
3345 ACTAGT
SspBI 2621 TGTACA T^GTACA
2882 TGTACA
StyI 1176 CCTTGG C^C[AT][AT]GG
3496 CCAAGG
XmnI 1943 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3231 GAACAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI AspHI AvaII
BanI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRI EcoRII
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI PflMI PleI PshAI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI SspI SwaI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XbaI XcmI