##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccI AccIII AgeI
AscI Asp718I AspI AviII AvrII
BbeI BbvCI BcgI BlnI BlpI
Bpu1102I BsaWI BseAI BsiWI BspDI
BspEI BsrBI BssHII Bst1107I BstAPI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraIII EagI EclXI Eco52I
EcoO65I FseI FspI HpaI KasI
KpnI KspI MluI MroI NarI
NotI NruI PacI PaeR7I PflFI
PinAI PmeI PstI PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SnaBI SpeI Sse8387I
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 2227 CGCG CG^CG
AclI 241 AACGTT AA^CGTT
AflII 4721 CTTAAG C^TTAAG
Alw44I 2824 GTGCAC G^TGCAC
Aor51HI 1014 AGCGCT AGC^GCT
ApaLI 2824 GTGCAC G^TGCAC
AseI 2527 ATTAAT AT^TAAT
AsnI 2527 ATTAAT AT^TAAT
AvaI 33 CCCGGG C^[CT]CG[GA]G
BbrPI 693 CACGTG CAC^GTG
BbsI 2064 GAAGAC GAAGAC(2/6)
BfrI 4721 CTTAAG C^TTAAG
BpuAI 2064 GAAGAC GAAGAC(2/6)
BsaAI 693 CACGTG [CT]AC^GT[GA]
BsaBI 189 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2129 CGACCG CG[GA][CT]^CG
BsoBI 33 CCCGGG C^[CT]CG[GA]G
BstBI 2805 TTCGAA TT^CGAA
BstUI 2227 CGCG CG^CG
CfoI 1015 GCGC GCG^C
DrdI 1473 GACCTTCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1014 AGCGCT AGC^GCT
EcoRV 2520 GATATC GAT^ATC
EcoT22I 3600 ATGCAT ATGCA^T
HaeII 1014 AGCGCT [GA]GCGC^[CT]
HhaI 1015 GCGC GCG^C
HinP1I 1015 GCGC G^CGC
HincII 2440 GTTGAC GT[CT]^[GA]AC
HindII 2440 GTTGAC GT[CT]^[GA]AC
MamI 189 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2227 CGCG CG^CG
NaeI 757 GCCGGC GCC^GGC
NdeI 1713 CATATG CA^TATG
NgoMIV 757 GCCGGC G^CCGGC
NsiI 3600 ATGCAT ATGCA^T
NspV 2805 TTCGAA TT^CGAA
PflMI 1279 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 693 CACGTG CAC^GTG
PmlI 693 CACGTG CAC^GTG
PshAI 4659 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2527 ATTAAT AT^TAAT
Psp1406I 241 AACGTT AA^CGTT
PvuII 3455 CAGCTG CAG^CTG
SacI 2337 GAGCTC GAGCT^C
SapI 3936 GCTCTTC GCTCTTC(1/4)
SexAI 1141 ACCTGGT A^CC[AT]GGT
SfuI 2805 TTCGAA TT^CGAA
SmaI 33 CCCGGG CCC^GGG
SphI 2885 GCATGC GCATG^C
SwaI 4317 ATTTAAAT ATTT^AAAT
Van91I 1279 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2760 TCTAGA T^CTAGA
XmaCI 33 CCCGGG C^CCGGG
XmaI 33 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 847 GGCGTC G[GA]^CG[CT]C
2848 GGCGTC
AhdI 1569 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3472 GACTCTCAGTC
AocI 1821 CCTAAGG CC^T[ACGT]AGG
3322 CCTCAGG
ApaI 38 GGGCCC GGGCC^C
719 GGGCCC
AspEI 1569 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3472 GACTCTCAGTC
BalI 967 TGGCCA TGG^CCA
1174 TGGCCA
BamHI 1787 GGATCC G^GATCC
2857 GGATCC
BciVI 1538 GGATAC GTATCC(6/5)
3539 GTATCC
BclI 2368 TGATCA T^GATCA
3696 TGATCA
BglI 752 GCCTGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
765 GCCCAGAAGGC
BsaHI 847 GGCGTC G[GA]^CG[CT]C
2848 GGCGTC
BsmBI 1041 GAGACG CGTCTC(1/5)
2125 GAGACG
BsmI 631 GAATGC GAATGC(1/-1)
2783 GAATGC
BspHI 2261 TCATGA T^CATGA
3865 TCATGA
BspMI 1021 ACCTGC ACCTGC(4/8)
3932 ACCTGC
BsrDI 299 GCAATG GCAATG(2/0)
3977 GCAATG
BsrFI 757 GCCGGC [GA]^CCGG[CT]
2131 ACCGGC
BstXI 1252 CCAAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4269 CCAACTCATTGG
Bsu36I 1821 CCTAAGG CC^T[ACGT]AGG
3322 CCTCAGG
Cfr10I 757 GCCGGC [GA]^CCGG[CT]
2131 ACCGGC
Eam1105I 1569 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3472 GACTCTCAGTC
Eco81I 1821 CCTAAGG CC^T[ACGT]AGG
3322 CCTCAGG
EcoNI 2647 CCTAATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3507 CCTAGAGCAGG
FbaI 2368 TGATCA T^GATCA
3696 TGATCA
Hin1I 847 GGCGTC G[GA]^CG[CT]C
2848 GGCGTC
HindIII 3273 AAGCTT A^AGCTT
4307 AAGCTT
MfeI 673 CAATTG C^AATTG
1450 CAATTG
MluNI 967 TGGCCA TGG^CCA
1174 TGGCCA
MscI 967 TGGCCA TGG^CCA
1174 TGGCCA
MunI 673 CAATTG C^AATTG
1450 CAATTG
NcoI 3304 CCATGG C^CATGG
3484 CCATGG
NheI 468 GCTAGC G^CTAGC
761 GCTAGC
PpuMI 1281 AGGACCT [GA]G^G[AT]CC[CT]
1692 GGGACCT
RcaI 2261 TCATGA T^CATGA
3865 TCATGA
StuI 926 AGGCCT AGG^CCT
2644 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanI BanII BbvI BcnI
BfaI BglII BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspLU11I BsrGI BsrI BssKI BssSI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI