##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI Alw44I AocI ApaLI AscI
Asp718I AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsiEI BsiWI BsmBI
BspMI BsrFI BssHII BstAPI BstEII
BstPI Bsu36I CelII Cfr10I CpoI
DraIII DrdI EagI EclXI Eco52I
Eco81I EcoO65I FseI FspI Hin1I
HpaI KasI KpnI MluI NaeI
NarI NcoI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SalI SbfI
SexAI SfiI SfoI SgrAI SmaI
SmlI Sse8387I SwaI Tth111I Van91I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 246 TCCGGA T^CCGGA
Aor51HI 34 AGCGCT AGC^GCT
ApaI 1310 GGGCCC GGGCC^C
AvaI 1183 CCCGAG C^[CT]CG[GA]G
BanI 18 GGTGCC G^G[CT][GA]CC
BsaAI 2912 TACGTA [CT]AC^GT[GA]
BsaI 3663 GGTCTC GGTCTC(1/5)
BseAI 246 TCCGGA T^CCGGA
BsoBI 1183 CCCGAG C^[CT]CG[GA]G
BspDI 2762 ATCGAT AT^CGAT
BspEI 246 TCCGGA T^CCGGA
BsrBI 2128 CCGCTC CCGCTC(-3/-3)
BsrDI 2167 CATTGC GCAATG(2/0)
BssSI 2998 CACGAG CACGAG(-5/-1)
BstBI 834 TTCGAA TT^CGAA
ClaI 2762 ATCGAT AT^CGAT
Eco47III 34 AGCGCT AGC^GCT
EcoNI 3115 CCTTATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3519 GATATC GAT^ATC
FauI 1819 GCGGG CCCGC(4/6)
HgaI 729 GACGC GACGC(5/10)
HindIII 492 AAGCTT A^AGCTT
KspI 22 CCGCGG CCGC^GG
MfeI 2802 CAATTG C^AATTG
MroI 246 TCCGGA T^CCGGA
MunI 2802 CAATTG C^AATTG
NdeI 2934 CATATG CA^TATG
NspV 834 TTCGAA TT^CGAA
PpuMI 1821 GGGACCC [GA]G^G[AT]CC[CT]
PstI 30 CTGCAG CTGCA^G
SacII 22 CCGCGG CCGC^GG
SapI 7 GAAGAGC GCTCTTC(1/4)
ScaI 3474 AGTACT AGT^ACT
SfuI 834 TTCGAA TT^CGAA
SnaBI 2912 TACGTA TAC^GTA
SpeI 1195 ACTAGT A^CTAGT
SphI 2642 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1522 GTATAC GT^[AC][GT]AC
4582 GTATAC
AhdI 2252 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3622 GACATTCTGTC
AlwNI 819 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
3083 CAGTTACTG
Asp700I 3743 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4516 GAAATTCTTC
AspEI 2252 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3622 GACATTCTGTC
BsaBI 416 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3128 GATAAAAATC
BsaWI 246 TCCGGA [AT]^CCGG[AT]
429 ACCGGA
BsgI 1769 CTGCAC GTGCAG(16/14)
1973 GTGCAG
BspHI 696 TCATGA T^CATGA
4524 TCATGA
Bst1107I 1522 GTATAC GTA^TAC
4582 GTATAC
BstXI 1780 CCACATCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1998 CCACTTTTCTGG
BstYI 299 GGATCT [GA]^GATC[CT]
556 AGATCC
BstZ17I 1522 GTATAC GTA^TAC
4582 GTATAC
DsaI 22 CCGCGG C^C[GA][CT]GG
2264 CCGTGG
Eam1105I 2252 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3622 GACATTCTGTC
EarI 4272 CTCTTC CTCTTC(1/4)
7 GAAGAG
EcoT22I 2644 ATGCAT ATGCA^T
2931 ATGCAT
HaeII 34 AGCGCT [GA]GCGC^[CT]
635 GGCGCT
HincII 1067 GTCAAC GT[CT]^[GA]AC
3539 GTCAAC
HindII 1067 GTCAAC GT[CT]^[GA]AC
3539 GTCAAC
Ksp632I 4272 CTCTTC CTCTTC(1/4)
7 GAAGAG
MamI 416 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3128 GATAAAAATC
MflI 299 GGATCT [GA]^GATC[CT]
556 AGATCC
MwoI 12 GCTCCGGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
2380 GCAACATCAGC
NsiI 2644 ATGCAT ATGCA^T
2931 ATGCAT
PvuII 1322 CAGCTG CAG^CTG
1962 CAGCTG
RcaI 696 TCATGA T^CATGA
4524 TCATGA
SacI 10 GAGCTC GAGCT^C
1649 GAGCTC
SspI 470 AATATT AAT^ATT
2493 AATATT
StuI 1487 AGGCCT AGG^CCT
4114 AGGCCT
XbaI 3594 TCTAGA T^CTAGA
4673 TCTAGA
XhoII 299 GGATCT [GA]^GATC[CT]
556 AGATCC
XmnI 3743 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4516 GAAATTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AspHI AvaII BalI BanII BbsI
BbvI BclI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspLU11I BsrGI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EcoO109I EcoRI EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI NciI NdeII NlaIII NlaIV
NspI PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I