##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AflII AgeI AscI
AseI AsnI Asp700I Asp718I AviII
BfrI BsaBI BsiEI BsiWI BsmBI
BsrBI BssHII BssSI Bst1107I BstBI
BstZ17I CpoI EagI EclXI Eco52I
EcoRV EcoT22I FseI FspI HindIII
HpaI KpnI MamI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshBI PvuI RsrII SbfI SexAI
SfiI SfuI SgrAI SnaBI Sse8387I
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 611 GTCGAC GT^[AC][GT]AC
AccIII 315 TCCGGA T^CCGGA
AclI 3844 AACGTT AA^CGTT
AhdI 3977 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1527 GTGCAC G^TGCAC
Aor51HI 3967 AGCGCT AGC^GCT
ApaI 887 GGGCCC GGGCC^C
ApaLI 1527 GTGCAC G^TGCAC
AspEI 3977 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3927 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 502 CCTAGG C^CTAGG
BamHI 874 GGATCC G^GATCC
BbrPI 3917 CACGTG CAC^GTG
BglI 2904 GCCCCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 502 CCTAGG C^CTAGG
BlpI 812 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 812 GCTGAGC GC^T[ACGT]AGC
BsaWI 315 TCCGGA [AT]^CCGG[AT]
BseAI 315 TCCGGA T^CCGGA
Bsp1407I 2964 TGTACA T^GTACA
BspDI 2321 ATCGAT AT^CGAT
BspEI 315 TCCGGA T^CCGGA
BspMI 273 ACCTGC ACCTGC(4/8)
BsrGI 2964 TGTACA T^GTACA
BstEII 107 GGTCACC G^GT[ACGT]ACC
BstPI 107 GGTCACC G^GT[ACGT]ACC
CelII 812 GCTGAGC GC^T[ACGT]AGC
ClaI 2321 ATCGAT AT^CGAT
DrdI 2689 GACCCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3977 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3967 AGCGCT AGC^GCT
EcoO65I 107 GGTCACC G^GT[ACGT]ACC
EcoRI 1411 GAATTC G^AATTC
KspI 3646 CCGCGG CCGC^GG
MroI 315 TCCGGA T^CCGGA
NaeI 3776 GCCGGC GCC^GGC
NgoMIV 3776 GCCGGC G^CCGGC
PaeR7I 2988 CTCGAG C^TCGAG
PflFI 3927 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1141 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3917 CACGTG CAC^GTG
PmlI 3917 CACGTG CAC^GTG
PshAI 3178 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3844 AACGTT AA^CGTT
SacII 3646 CCGCGG CCGC^GG
SalI 611 GTCGAC G^TCGAC
SapI 3494 GAAGAGC GCTCTTC(1/4)
SpeI 4591 ACTAGT A^CTAGT
SphI 2787 GCATGC GCATG^C
SspBI 2964 TGTACA T^GTACA
SspI 2371 AATATT AAT^ATT
Tth111I 3927 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1141 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 4281 CCAAAAATTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2988 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 1411 GAATTC [GA]^AATT[CT]
4557 AAATTT
AcyI 262 GGCGCC G[GA]^CG[CT]C
3779 GGCGCC
AocI 242 CCTGAGG CC^T[ACGT]AGG
2731 CCTCAGG
ApoI 1411 GAATTC [GA]^AATT[CT]
4557 AAATTT
BalI 285 TGGCCA TGG^CCA
1932 TGGCCA
BbeI 262 GGCGCC GGCGC^C
3779 GGCGCC
BbsI 743 GAAGAC GAAGAC(2/6)
3175 GAAGAC
BbvCI 3721 CCTCAGC CCTCAGC(-5/-2)
1279 GCTGAGG
BcgI 1093 CGATAAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
540 GCACATCATTCG
BciVI 1239 GTATCC GTATCC(6/5)
3698 GTATCC
BclI 2053 TGATCA T^GATCA
4672 TGATCA
BpuAI 743 GAAGAC GAAGAC(2/6)
3175 GAAGAC
BsaAI 880 CACGTA [CT]AC^GT[GA]
3917 CACGTG
BsaHI 262 GGCGCC G[GA]^CG[CT]C
3779 GGCGCC
BspHI 412 TCATGA T^CATGA
3521 TCATGA
BsrDI 388 CATTGC GCAATG(2/0)
1536 GCAATG
BsrFI 1338 ACCGGC [GA]^CCGG[CT]
3776 GCCGGC
BstXI 216 CCAATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
513 CCAAGGAGCTGG
Bsu36I 242 CCTGAGG CC^T[ACGT]AGG
2731 CCTCAGG
Cfr10I 1338 ACCGGC [GA]^CCGG[CT]
3776 GCCGGC
DraIII 1105 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3656 CACATGGTG
Eco81I 242 CCTGAGG CC^T[ACGT]AGG
2731 CCTCAGG
EcoNI 3508 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3612 CCTGGAGCAGG
FbaI 2053 TGATCA T^GATCA
4672 TGATCA
HgaI 3754 GACGC GACGC(5/10)
66 GCGTC
Hin1I 262 GGCGCC G[GA]^CG[CT]C
3779 GGCGCC
HincII 611 GTCGAC GT[CT]^[GA]AC
2806 GTTGAC
HindII 611 GTCGAC GT[CT]^[GA]AC
2806 GTTGAC
KasI 262 GGCGCC G^GCGCC
3779 GGCGCC
MluNI 285 TGGCCA TGG^CCA
1932 TGGCCA
MscI 285 TGGCCA TGG^CCA
1932 TGGCCA
NarI 262 GGCGCC GG^CGCC
3779 GGCGCC
RcaI 412 TCATGA T^CATGA
3521 TCATGA
SfoI 262 GGCGCC GGC^GCC
3779 GGCGCC
StuI 3516 AGGCCT AGG^CCT
3568 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BglII BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspLU11I BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI