################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AatII Acc65I AflII AgeI AscI AseI AsnI Asp700I Asp718I AviII BfrI BsaBI BsiEI BsiWI BsmBI BsrBI BssHII BssSI Bst1107I BstBI BstZ17I CpoI EagI EclXI Eco52I EcoRV EcoT22I FseI FspI HindIII HpaI KpnI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshBI PvuI RsrII SbfI SexAI SfiI SfuI SgrAI SnaBI Sse8387I SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 611 GTCGAC GT^[AC][GT]AC AccIII 315 TCCGGA T^CCGGA AclI 3844 AACGTT AA^CGTT AhdI 3977 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1527 GTGCAC G^TGCAC Aor51HI 3967 AGCGCT AGC^GCT ApaI 887 GGGCCC GGGCC^C ApaLI 1527 GTGCAC G^TGCAC AspEI 3977 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3927 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 502 CCTAGG C^CTAGG BamHI 874 GGATCC G^GATCC BbrPI 3917 CACGTG CAC^GTG BglI 2904 GCCCCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 502 CCTAGG C^CTAGG BlpI 812 GCTGAGC GC^T[ACGT]AGC Bpu1102I 812 GCTGAGC GC^T[ACGT]AGC BsaWI 315 TCCGGA [AT]^CCGG[AT] BseAI 315 TCCGGA T^CCGGA Bsp1407I 2964 TGTACA T^GTACA BspDI 2321 ATCGAT AT^CGAT BspEI 315 TCCGGA T^CCGGA BspMI 273 ACCTGC ACCTGC(4/8) BsrGI 2964 TGTACA T^GTACA BstEII 107 GGTCACC G^GT[ACGT]ACC BstPI 107 GGTCACC G^GT[ACGT]ACC CelII 812 GCTGAGC GC^T[ACGT]AGC ClaI 2321 ATCGAT AT^CGAT DrdI 2689 GACCCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 3977 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3967 AGCGCT AGC^GCT EcoO65I 107 GGTCACC G^GT[ACGT]ACC EcoRI 1411 GAATTC G^AATTC KspI 3646 CCGCGG CCGC^GG MroI 315 TCCGGA T^CCGGA NaeI 3776 GCCGGC GCC^GGC NgoMIV 3776 GCCGGC G^CCGGC PaeR7I 2988 CTCGAG C^TCGAG PflFI 3927 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1141 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3917 CACGTG CAC^GTG PmlI 3917 CACGTG CAC^GTG PshAI 3178 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 3844 AACGTT AA^CGTT SacII 3646 CCGCGG CCGC^GG SalI 611 GTCGAC G^TCGAC SapI 3494 GAAGAGC GCTCTTC(1/4) SpeI 4591 ACTAGT A^CTAGT SphI 2787 GCATGC GCATG^C SspBI 2964 TGTACA T^GTACA SspI 2371 AATATT AAT^ATT Tth111I 3927 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1141 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 4281 CCAAAAATTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2988 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcsI 1411 GAATTC [GA]^AATT[CT] 4557 AAATTT AcyI 262 GGCGCC G[GA]^CG[CT]C 3779 GGCGCC AocI 242 CCTGAGG CC^T[ACGT]AGG 2731 CCTCAGG ApoI 1411 GAATTC [GA]^AATT[CT] 4557 AAATTT BalI 285 TGGCCA TGG^CCA 1932 TGGCCA BbeI 262 GGCGCC GGCGC^C 3779 GGCGCC BbsI 743 GAAGAC GAAGAC(2/6) 3175 GAAGAC BbvCI 3721 CCTCAGC CCTCAGC(-5/-2) 1279 GCTGAGG BcgI 1093 CGATAAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 540 GCACATCATTCG BciVI 1239 GTATCC GTATCC(6/5) 3698 GTATCC BclI 2053 TGATCA T^GATCA 4672 TGATCA BpuAI 743 GAAGAC GAAGAC(2/6) 3175 GAAGAC BsaAI 880 CACGTA [CT]AC^GT[GA] 3917 CACGTG BsaHI 262 GGCGCC G[GA]^CG[CT]C 3779 GGCGCC BspHI 412 TCATGA T^CATGA 3521 TCATGA BsrDI 388 CATTGC GCAATG(2/0) 1536 GCAATG BsrFI 1338 ACCGGC [GA]^CCGG[CT] 3776 GCCGGC BstXI 216 CCAATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 513 CCAAGGAGCTGG Bsu36I 242 CCTGAGG CC^T[ACGT]AGG 2731 CCTCAGG Cfr10I 1338 ACCGGC [GA]^CCGG[CT] 3776 GCCGGC DraIII 1105 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG 3656 CACATGGTG Eco81I 242 CCTGAGG CC^T[ACGT]AGG 2731 CCTCAGG EcoNI 3508 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3612 CCTGGAGCAGG FbaI 2053 TGATCA T^GATCA 4672 TGATCA HgaI 3754 GACGC GACGC(5/10) 66 GCGTC Hin1I 262 GGCGCC G[GA]^CG[CT]C 3779 GGCGCC HincII 611 GTCGAC GT[CT]^[GA]AC 2806 GTTGAC HindII 611 GTCGAC GT[CT]^[GA]AC 2806 GTTGAC KasI 262 GGCGCC G^GCGCC 3779 GGCGCC MluNI 285 TGGCCA TGG^CCA 1932 TGGCCA MscI 285 TGGCCA TGG^CCA 1932 TGGCCA NarI 262 GGCGCC GG^CGCC 3779 GGCGCC RcaI 412 TCATGA T^CATGA 3521 TCATGA SfoI 262 GGCGCC GGC^GCC 3779 GGCGCC StuI 3516 AGGCCT AGG^CCT 3568 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BglII BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI