##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AclI AgeI AscI BbrPI
BciVI BclI BglII BsaI BsiEI
BsiWI Bsp1407I BspDI BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI EagI EclXI Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
HindIII HpaI KspI MluI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SfuI
SgrAI SspBI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 2834 GGTACC G^GTACC
AccI 269 GTCGAC GT^[AC][GT]AC
AccIII 1235 TCCGGA T^CCGGA
AflIII 2798 ACATGT A^C[GA][CT]GT
AhdI 1980 GACCCGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2139 GTGCAC G^TGCAC
Aor51HI 1795 AGCGCT AGC^GCT
ApaLI 2139 GTGCAC G^TGCAC
Asp718I 2834 GGTACC G^GTACC
AspEI 1980 GACCCGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4692 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 614 TGCGCA TGC^GCA
BamHI 3828 GGATCC G^GATCC
BbeI 4574 GGCGCC GGCGC^C
BbsI 2089 GAAGAC GAAGAC(2/6)
BcgI 2046 GCAGCAGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 2089 GAAGAC GAAGAC(2/6)
BsaBI 2816 GATAACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1235 TCCGGA T^CCGGA
BsmBI 4697 CGTCTC CGTCTC(1/5)
BsmI 742 GAATGC GAATGC(1/-1)
BspEI 1235 TCCGGA T^CCGGA
BspLU11I 2798 ACATGT A^CATGT
BsrFI 2074 GCCGGC [GA]^CCGG[CT]
BssSI 4482 CACGAG CACGAG(-5/-1)
BstAPI 4142 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 3828 GGATCC [GA]^GATC[CT]
Cfr10I 2074 GCCGGC [GA]^CCGG[CT]
DrdI 3642 GACAGCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1980 GACCCGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1795 AGCGCT AGC^GCT
EcoRI 4122 GAATTC G^AATTC
FspI 614 TGCGCA TGC^GCA
HincII 269 GTCGAC GT[CT]^[GA]AC
HindII 269 GTCGAC GT[CT]^[GA]AC
KasI 4574 GGCGCC G^GCGCC
KpnI 2834 GGTACC GGTAC^C
MamI 2816 GATAACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2391 CAATTG C^AATTG
MflI 3828 GGATCC [GA]^GATC[CT]
MroI 1235 TCCGGA T^CCGGA
MunI 2391 CAATTG C^AATTG
NaeI 2074 GCCGGC GCC^GGC
NarI 4574 GGCGCC GG^CGCC
NgoMIV 2074 GCCGGC G^CCGGC
PflFI 4692 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 3087 GAGCTC GAGCT^C
SalI 269 GTCGAC G^TCGAC
SapI 4354 GCTCTTC GCTCTTC(1/4)
SbfI 876 CCTGCAGG CCTGCA^GG
ScaI 620 AGTACT AGT^ACT
SexAI 434 ACCTGGT A^CC[AT]GGT
SfiI 1032 GGCCCACAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4574 GGCGCC GGC^GCC
SnaBI 2609 TACGTA TAC^GTA
SpeI 1456 ACTAGT A^CTAGT
SphI 162 GCATGC GCATG^C
Sse8387I 876 CCTGCAGG CCTGCA^GG
Tth111I 4692 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 3828 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 266 GGCGTC G[GA]^CG[CT]C
4574 GGCGCC
AflII 229 CTTAAG C^TTAAG
4405 CTTAAG
AseI 2426 ATTAAT AT^TAAT
2551 ATTAAT
AsnI 2426 ATTAAT AT^TAAT
2551 ATTAAT
BfrI 229 CTTAAG C^TTAAG
4405 CTTAAG
BlpI 3571 GCTTAGC GC^T[ACGT]AGC
3879 GCTGAGC
Bpu1102I 3571 GCTTAGC GC^T[ACGT]AGC
3879 GCTGAGC
BsaAI 946 TACGTG [CT]AC^GT[GA]
2609 TACGTA
BsaHI 266 GGCGTC G[GA]^CG[CT]C
4574 GGCGCC
BsaWI 461 TCCGGT [AT]^CCGG[AT]
1235 TCCGGA
BspHI 1976 TCATGA T^CATGA
2631 TCATGA
BsrBI 2177 CCGCTC CCGCTC(-3/-3)
805 GAGCGG
BssHII 40 GCGCGC G^CGCGC
338 GCGCGC
CelII 3571 GCTTAGC GC^T[ACGT]AGC
3879 GCTGAGC
EcoNI 1510 CCTCCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2453 CCTCTACAAGG
HaeII 1795 AGCGCT [GA]GCGC^[CT]
4574 GGCGCC
Hin1I 266 GGCGTC G[GA]^CG[CT]C
4574 GGCGCC
NheI 580 GCTAGC G^CTAGC
990 GCTAGC
NspI 162 GCATGC [GA]CATG^[CT]
2798 ACATGT
PshBI 2426 ATTAAT AT^TAAT
2551 ATTAAT
RcaI 1976 TCATGA T^CATGA
2631 TCATGA
SmaI 2134 CCCGGG CCC^GGG
4625 CCCGGG
SmlI 229 CTTAAG C^T[CT][GA]AG
4405 CTTAAG
XbaI 3734 TCTAGA T^CTAGA
4012 TCTAGA
XmaCI 2134 CCCGGG C^CCGGG
4625 CCCGGG
XmaI 2134 CCCGGG C^CCGGG
4625 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
Asp700I AspHI AvaI AvaII AvrII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BglI BlnI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XmnI