##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AhdI Alw44I Aor51HI
ApaLI AscI Asp718I AspEI AspI
BamHI BbeI BbrPI BsiEI BsiWI
BspDI BspLU11I BssHII BstBI ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV FauI FseI KasI
KpnI KspI MluI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 2015 CGCG CG^CG
AccIII 2484 TCCGGA T^CCGGA
AclI 2555 AACGTT AA^CGTT
AcyI 1038 GACGCC G[GA]^CG[CT]C
AflII 1100 CTTAAG C^TTAAG
AgeI 3701 ACCGGT A^CCGGT
ApaI 769 GGGCCC GGGCC^C
AviII 4616 TGCGCA TGC^GCA
BcgI 3144 CGACTCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1100 CTTAAG C^TTAAG
BglI 1290 GCCTGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 6448 AGATCT A^GATCT
BlpI 1841 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1841 GCTCAGC GC^T[ACGT]AGC
BsaAI 4239 CACGTA [CT]AC^GT[GA]
BsaBI 6180 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1038 GACGCC G[GA]^CG[CT]C
BseAI 2484 TCCGGA T^CCGGA
BspEI 2484 TCCGGA T^CCGGA
BspHI 5367 TCATGA T^CATGA
BsrBI 43 CCGCTC CCGCTC(-3/-3)
BssSI 2039 CTCGTG CACGAG(-5/-1)
Bst1107I 7028 GTATAC GTA^TAC
BstEII 6597 GGTGACC G^GT[ACGT]ACC
BstPI 6597 GGTGACC G^GT[ACGT]ACC
BstUI 2015 CGCG CG^CG
BstZ17I 7028 GTATAC GTA^TAC
CelII 1841 GCTCAGC GC^T[ACGT]AGC
DraIII 6222 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 6597 GGTGACC G^GT[ACGT]ACC
FspI 4616 TGCGCA TGC^GCA
HaeII 419 GGCGCT [GA]GCGC^[CT]
Hin1I 1038 GACGCC G[GA]^CG[CT]C
HindIII 4089 AAGCTT A^AGCTT
HpaI 4125 GTTAAC GTT^AAC
MamI 6180 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2484 TCCGGA T^CCGGA
MvnI 2015 CGCG CG^CG
PinAI 3701 ACCGGT A^CCGGT
Psp1406I 2555 AACGTT AA^CGTT
RcaI 5367 TCATGA T^CATGA
SacI 2588 GAGCTC GAGCT^C
SspI 2655 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 15 GTAGAC GT^[AC][GT]AC
7028 GTATAC
AflIII 4238 ACACGT A^C[GA][CT]GT
7133 ACGTGT
AocI 4330 CCTCAGG CC^T[ACGT]AGG
6583 CCTCAGG
AvrII 1652 CCTAGG C^CTAGG
3073 CCTAGG
BbvCI 3465 CCTCAGC CCTCAGC(-5/-2)
3677 GCTGAGG
BlnI 1652 CCTAGG C^CTAGG
3073 CCTAGG
Bsp1407I 2434 TGTACA T^GTACA
6379 TGTACA
BsrDI 1494 CATTGC GCAATG(2/0)
5110 GCAATG
BsrFI 3701 ACCGGT [GA]^CCGG[CT]
6576 ACCGGC
BsrGI 2434 TGTACA T^GTACA
6379 TGTACA
Bsu36I 4330 CCTCAGG CC^T[ACGT]AGG
6583 CCTCAGG
Cfr10I 3701 ACCGGT [GA]^CCGG[CT]
6576 ACCGGC
DrdI 2723 GACACAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6600 GACCAGGTTGTC
Eco81I 4330 CCTCAGG CC^T[ACGT]AGG
6583 CCTCAGG
EcoRI 493 GAATTC G^AATTC
1250 GAATTC
EcoT22I 2114 ATGCAT ATGCA^T
4556 ATGCAT
MfeI 6944 CAATTG C^AATTG
7097 CAATTG
MunI 6944 CAATTG C^AATTG
7097 CAATTG
NcoI 3321 CCATGG C^CATGG
4696 CCATGG
NsiI 2114 ATGCAT ATGCA^T
4556 ATGCAT
PshAI 6204 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
7256 GACAAGTGTC
SapI 2375 GAAGAGC GCTCTTC(1/4)
4205 GAAGAGC
SexAI 5598 ACCTGGT A^CC[AT]GGT
6601 ACCAGGT
SmaI 255 CCCGGG CCC^GGG
766 CCCGGG
SpeI 4001 ACTAGT A^CTAGT
6456 ACTAGT
SspBI 2434 TGTACA T^GTACA
6379 TGTACA
StuI 4654 AGGCCT AGG^CCT
5350 AGGCCT
XmaCI 255 CCCGGG C^CCGGG
766 CCCGGG
XmaI 255 CCCGGG C^CCGGG
766 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BciVI BclI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII
XmnI