################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII AccIII AflII AgeI Alw44I ApaI ApaLI AscI AseI AsnI AvaI AviII AvrII BbeI BbrPI BcnI BfrI BglI BlnI BsaAI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspMI BsrFI Bst1107I BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco52I EcoO65I EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KspI MluI MroI NaeI NarI NciI NgoMIV NotI NsiI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 476 GGTACC G^GTACC AccI 3883 GTAGAC GT^[AC][GT]AC AclI 3853 AACGTT AA^CGTT Aor51HI 828 AGCGCT AGC^GCT Asp700I 4344 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 476 GGTACC G^GTACC AspI 5303 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2266 TGGCCA TGG^CCA BamHI 1904 GGATCC G^GATCC BbvCI 2409 GCTGAGG CCTCAGC(-5/-2) BcgI 355 CGAGCAGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1858 TGATCA T^GATCA BglII 4155 AGATCT A^GATCT BlpI 3202 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3202 GCTCAGC GC^T[ACGT]AGC BsaBI 4528 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 3403 ACCGGA [AT]^CCGG[AT] BsgI 2254 GTGCAG GTGCAG(16/14) BspLU11I 3537 ACATGT A^CATGT BsrBI 3143 GAGCGG CCGCTC(-3/-3) BssSI 2422 CTCGTG CACGAG(-5/-1) BstBI 1339 TTCGAA TT^CGAA CelII 3202 GCTCAGC GC^T[ACGT]AGC DraI 4332 TTTAAA TTT^AAA Eco47III 828 AGCGCT AGC^GCT EcoRV 1245 GATATC GAT^ATC FbaI 1858 TGATCA T^GATCA KpnI 476 GGTACC GGTAC^C MamI 4528 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3248 CAATTG C^AATTG MluNI 2266 TGGCCA TGG^CCA MscI 2266 TGGCCA TGG^CCA MunI 3248 CAATTG C^AATTG NheI 4110 GCTAGC G^CTAGC NruI 75 TCGCGA TCG^CGA NspV 1339 TTCGAA TT^CGAA PacI 3057 TTAATTAA TTAAT^TAA PflFI 5303 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 3853 AACGTT AA^CGTT SacI 3200 GAGCTC GAGCT^C SapI 2010 GAAGAGC GCTCTTC(1/4) ScaI 4514 AGTACT AGT^ACT SfuI 1339 TTCGAA TT^CGAA SpeI 97 ACTAGT A^CTAGT SspI 5377 AATATT AAT^ATT StuI 3389 AGGCCT AGG^CCT Tth111I 5303 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 3465 CCATATAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 4344 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflIII 3190 ACGTGT A^C[GA][CT]GT 3537 ACATGT AhdI 301 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1384 GACGTAGAGTC AocI 3092 CCTGAGG CC^T[ACGT]AGG 4900 CCTCAGG AspEI 301 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1384 GACGTAGAGTC BanI 476 GGTACC G^G[CT][GA]CC 5195 GGTGCC BanII 3200 GAGCTC G[GA]GC[CT]^C 4937 GAGCCC BsaI 375 GGTCTC GGTCTC(1/5) 2237 GGTCTC BsmI 4695 GAATGC GAATGC(1/-1) 4348 GCATTC BssHII 257 GCGCGC G^CGCGC 259 GCGCGC Bsu36I 3092 CCTGAGG CC^T[ACGT]AGG 4900 CCTCAGG DraII 1430 AGGACCT [GA]G^G[ACGT]CC[CT] 2033 AGGACCC Eam1105I 301 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1384 GACGTAGAGTC Eco81I 3092 CCTGAGG CC^T[ACGT]AGG 4900 CCTCAGG EcoNI 33 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2569 CCTCATTAAGG EcoO109I 1430 AGGACCT [GA]G^G[ACGT]CC[CT] 2033 AGGACCC EcoRI 1342 GAATTC G^AATTC 2319 GAATTC PpuMI 1430 AGGACCT [GA]G^G[AT]CC[CT] 2033 AGGACCC PstI 611 CTGCAG CTGCA^G 4816 CTGCAG XbaI 546 TCTAGA T^CTAGA 875 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BbsI BbvI BciVI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspHI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NcoI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII