##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccIII AflII AgeI Alw44I
ApaI ApaLI AscI AseI AsnI
AvaI AviII AvrII BbeI BbrPI
BcnI BfrI BglI BlnI BsaAI
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspMI BsrFI Bst1107I
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraIII DrdI EagI EclXI
Eco52I EcoO65I EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KspI MluI MroI NaeI NarI
NciI NgoMIV NotI NsiI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 476 GGTACC G^GTACC
AccI 3883 GTAGAC GT^[AC][GT]AC
AclI 3853 AACGTT AA^CGTT
Aor51HI 828 AGCGCT AGC^GCT
Asp700I 4344 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 476 GGTACC G^GTACC
AspI 5303 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2266 TGGCCA TGG^CCA
BamHI 1904 GGATCC G^GATCC
BbvCI 2409 GCTGAGG CCTCAGC(-5/-2)
BcgI 355 CGAGCAGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1858 TGATCA T^GATCA
BglII 4155 AGATCT A^GATCT
BlpI 3202 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3202 GCTCAGC GC^T[ACGT]AGC
BsaBI 4528 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3403 ACCGGA [AT]^CCGG[AT]
BsgI 2254 GTGCAG GTGCAG(16/14)
BspLU11I 3537 ACATGT A^CATGT
BsrBI 3143 GAGCGG CCGCTC(-3/-3)
BssSI 2422 CTCGTG CACGAG(-5/-1)
BstBI 1339 TTCGAA TT^CGAA
CelII 3202 GCTCAGC GC^T[ACGT]AGC
DraI 4332 TTTAAA TTT^AAA
Eco47III 828 AGCGCT AGC^GCT
EcoRV 1245 GATATC GAT^ATC
FbaI 1858 TGATCA T^GATCA
KpnI 476 GGTACC GGTAC^C
MamI 4528 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3248 CAATTG C^AATTG
MluNI 2266 TGGCCA TGG^CCA
MscI 2266 TGGCCA TGG^CCA
MunI 3248 CAATTG C^AATTG
NheI 4110 GCTAGC G^CTAGC
NruI 75 TCGCGA TCG^CGA
NspV 1339 TTCGAA TT^CGAA
PacI 3057 TTAATTAA TTAAT^TAA
PflFI 5303 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 3853 AACGTT AA^CGTT
SacI 3200 GAGCTC GAGCT^C
SapI 2010 GAAGAGC GCTCTTC(1/4)
ScaI 4514 AGTACT AGT^ACT
SfuI 1339 TTCGAA TT^CGAA
SpeI 97 ACTAGT A^CTAGT
SspI 5377 AATATT AAT^ATT
StuI 3389 AGGCCT AGG^CCT
Tth111I 5303 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3465 CCATATAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 4344 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 3190 ACGTGT A^C[GA][CT]GT
3537 ACATGT
AhdI 301 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1384 GACGTAGAGTC
AocI 3092 CCTGAGG CC^T[ACGT]AGG
4900 CCTCAGG
AspEI 301 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1384 GACGTAGAGTC
BanI 476 GGTACC G^G[CT][GA]CC
5195 GGTGCC
BanII 3200 GAGCTC G[GA]GC[CT]^C
4937 GAGCCC
BsaI 375 GGTCTC GGTCTC(1/5)
2237 GGTCTC
BsmI 4695 GAATGC GAATGC(1/-1)
4348 GCATTC
BssHII 257 GCGCGC G^CGCGC
259 GCGCGC
Bsu36I 3092 CCTGAGG CC^T[ACGT]AGG
4900 CCTCAGG
DraII 1430 AGGACCT [GA]G^G[ACGT]CC[CT]
2033 AGGACCC
Eam1105I 301 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1384 GACGTAGAGTC
Eco81I 3092 CCTGAGG CC^T[ACGT]AGG
4900 CCTCAGG
EcoNI 33 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2569 CCTCATTAAGG
EcoO109I 1430 AGGACCT [GA]G^G[ACGT]CC[CT]
2033 AGGACCC
EcoRI 1342 GAATTC G^AATTC
2319 GAATTC
PpuMI 1430 AGGACCT [GA]G^G[AT]CC[CT]
2033 AGGACCC
PstI 611 CTGCAG CTGCA^G
4816 CTGCAG
XbaI 546 TCTAGA T^CTAGA
875 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BbsI BbvI BciVI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspHI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NcoI NdeI
NdeII NlaIII NlaIV NspI PflMI
PleI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII