##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BanI BbeI BbrPI BcgI
BciVI BfrI BlnI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraI Eam1105I Eco47III EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
HaeII HindIII HpaI KasI KpnI
KspI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tsp509I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 1725 GACGTC GACGT^C
AflIII 1022 ACATGT A^C[GA][CT]GT
Alw44I 261 GTGCAC G^TGCAC
AocI 1137 CCTGAGG CC^T[ACGT]AGG
ApaLI 261 GTGCAC G^TGCAC
AviII 1582 TGCGCA TGC^GCA
BamHI 2059 GGATCC G^GATCC
BbsI 762 GAAGAC GAAGAC(2/6)
BbvCI 1239 GCTGAGG CCTCAGC(-5/-2)
BclI 967 TGATCA T^GATCA
BglI 1740 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 979 AGATCT A^GATCT
BlpI 1116 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1116 GCTCAGC GC^T[ACGT]AGC
BpuAI 762 GAAGAC GAAGAC(2/6)
BsaAI 258 TACGTG [CT]AC^GT[GA]
BsaBI 1187 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1491 CTGCAC GTGCAG(16/14)
BsiEI 13 CGGCCG CG[GA][CT]^CG
BspLU11I 1022 ACATGT A^CATGT
BspMI 1489 ACCTGC ACCTGC(4/8)
BsrDI 67 GCAATG GCAATG(2/0)
BssSI 354 CACGAG CACGAG(-5/-1)
Bsu36I 1137 CCTGAGG CC^T[ACGT]AGG
CelII 1116 GCTCAGC GC^T[ACGT]AGC
DraIII 384 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1754 GACTGTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 13 CGGCCG C^GGCCG
EclXI 13 CGGCCG C^GGCCG
Eco52I 13 CGGCCG C^GGCCG
Eco81I 1137 CCTGAGG CC^T[ACGT]AGG
FbaI 967 TGATCA T^GATCA
FspI 1582 TGCGCA TGC^GCA
HincII 2141 GTCAAC GT[CT]^[GA]AC
HindII 2141 GTCAAC GT[CT]^[GA]AC
MamI 1187 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1333 TTAA T^TAA
NcoI 541 CCATGG C^CATGG
PflMI 1715 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1635 CTGCAG CTGCA^G
SacI 810 GAGCTC GAGCT^C
SapI 1508 GCTCTTC GCTCTTC(1/4)
ScaI 508 AGTACT AGT^ACT
SexAI 850 ACCTGGT A^CC[AT]GGT
SfcI 1635 CTGCAG C^T[GA][CT]AG
SmaI 2048 CCCGGG CCC^GGG
Tru9I 1333 TTAA T^TAA
Van91I 1715 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2203 TCTAGA T^CTAGA
XmaCI 2048 CCCGGG C^CCGGG
XmaI 2048 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccIII 514 TCCGGA T^CCGGA
1473 TCCGGA
AcyI 1245 GACGCC G[GA]^CG[CT]C
1725 GACGTC
ApaI 1435 GGGCCC GGGCC^C
1647 GGGCCC
AspI 600 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
849 GACCTGGTC
AvaI 472 CCCGAG C^[CT]CG[GA]G
2048 CCCGGG
BsaHI 1245 GACGCC G[GA]^CG[CT]C
1725 GACGTC
BsaI 1477 GAGACC GGTCTC(1/5)
1056 GGTCTC
BsaWI 514 TCCGGA [AT]^CCGG[AT]
1473 TCCGGA
BseAI 514 TCCGGA T^CCGGA
1473 TCCGGA
BsoBI 472 CCCGAG C^[CT]CG[GA]G
2048 CCCGGG
BspEI 514 TCCGGA T^CCGGA
1473 TCCGGA
DsaI 541 CCATGG C^C[GA][CT]GG
1573 CCACGG
HgaI 1245 GACGC GACGC(5/10)
778 GCGTC
Hin1I 1245 GACGCC G[GA]^CG[CT]C
1725 GACGTC
MroI 514 TCCGGA T^CCGGA
1473 TCCGGA
PflFI 600 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
849 GACCTGGTC
TaqI 503 TCGA T^CGA
1090 TCGA
Tth111I 600 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
849 GACCTGGTC
TthHB8I 503 TCGA T^CGA
1090 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BalI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TseI Tsp45I
TspRI XcmI XhoII