################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AccIII AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BglI BlnI BsaHI BsaWI BseAI BsiEI BsiWI BspDI BspEI BsrFI BssHII BssSI BstBI BstEII BstPI Bsu36I Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I FauI FseI FspI HgaI Hin1I HpaI KasI KpnI MluI MroI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshBI PvuI RsrII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI Sse8387I StuI SwaI Van91I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 75 CGCG CG^CG AclI 4313 AACGTT AA^CGTT AflII 3878 CTTAAG C^TTAAG AspHI 1427 GAGCTC G[AT]GC[AT]^C AspI 3143 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 3754 CTCGAG C^[CT]CG[GA]G BbvCI 3213 GCTGAGG CCTCAGC(-5/-2) BcgI 1792 CGAACACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 3878 CTTAAG C^TTAAG BlpI 3519 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3519 GCTGAGC GC^T[ACGT]AGC BsaAI 3389 TACGTA [CT]AC^GT[GA] BsaBI 3438 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2273 GAGACC GGTCTC(1/5) BsiHKAI 1427 GAGCTC G[AT]GC[AT]^C BsmBI 979 GAGACG CGTCTC(1/5) BsoBI 3754 CTCGAG C^[CT]CG[GA]G BsrBI 18 GAGCGG CCGCTC(-3/-3) Bst1107I 4193 GTATAC GTA^TAC BstAPI 2089 GCAACTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 75 CGCG CG^CG BstZ17I 4193 GTATAC GTA^TAC CelII 3519 GCTGAGC GC^T[ACGT]AGC CfoI 2064 GCGC GCG^C EcoRI 1369 GAATTC G^AATTC EcoRV 3186 GATATC GAT^ATC EcoT22I 3403 ATGCAT ATGCA^T HaeII 2063 AGCGCC [GA]GCGC^[CT] HhaI 2064 GCGC GCG^C HinP1I 2064 GCGC G^CGC KspI 74 CCGCGG CCGC^GG MamI 3438 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 75 CGCG CG^CG NcoI 244 CCATGG C^CATGG NdeI 3169 CATATG CA^TATG NsiI 3403 ATGCAT ATGCA^T PaeR7I 3754 CTCGAG C^TCGAG PflFI 3143 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 4313 AACGTT AA^CGTT PvuII 3517 CAGCTG CAG^CTG SacI 1427 GAGCTC GAGCT^C SacII 74 CCGCGG CCGC^GG SapI 3813 GCTCTTC GCTCTTC(1/4) ScaI 1589 AGTACT AGT^ACT SnaBI 3389 TACGTA TAC^GTA SpeI 252 ACTAGT A^CTAGT SphI 3154 GCATGC GCATG^C Tth111I 3143 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 306 TCTAGA T^CTAGA XhoI 3754 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 4193 GTATAC GT^[AC][GT]AC 4199 GTCTAC AflIII 821 ACATGT A^C[GA][CT]GT 2866 ACATGT BsgI 1515 GTGCAG GTGCAG(16/14) 1800 GTGCAG BsmFI 7 GGGAC GGGAC(10/14) 96 GGGAC BspHI 2243 TCATGA T^CATGA 3341 TCATGA BspLU11I 821 ACATGT A^CATGT 2866 ACATGT BspMI 1248 ACCTGC ACCTGC(4/8) 1802 GCAGGT BsrDI 2196 CATTGC GCAATG(2/0) 2553 CATTGC BstXI 625 CCAATGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2002 CCAGTTGTGTGG DraI 1492 TTTAAA TTT^AAA 4755 TTTAAA DsaI 74 CCGCGG C^C[GA][CT]GG 244 CCATGG EcoNI 1417 CCTACTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1707 CCTGAAGGAGG HincII 1637 GTCAAC GT[CT]^[GA]AC 2575 GTCAAC HindII 1637 GTCAAC GT[CT]^[GA]AC 2575 GTCAAC HindIII 1701 AAGCTT A^AGCTT 4654 AAGCTT MfeI 523 CAATTG C^AATTG 2971 CAATTG MslI 2538 CATTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4020 CATGAAGATG MspA1I 74 CCGCGG C[AC]G^C[GT]G 3517 CAGCTG MunI 523 CAATTG C^AATTG 2971 CAATTG PshAI 521 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 868 GACTGCAGTC RcaI 2243 TCATGA T^CATGA 3341 TCATGA ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AvaII BalI BanII BbsI BbvI BciVI BclI BcnI BfaI BglII BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI