##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BanI
BbeI BbrPI BglI BlnI BsaHI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BsrFI BssHII BssSI BstBI
BstEII BstPI Bsu36I Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
FauI FseI FspI HgaI Hin1I
HpaI KasI KpnI MluI MroI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PshBI PvuI
RsrII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI Sse8387I
StuI SwaI Van91I XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 75 CGCG CG^CG
AclI 4313 AACGTT AA^CGTT
AflII 3878 CTTAAG C^TTAAG
AspHI 1427 GAGCTC G[AT]GC[AT]^C
AspI 3143 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3754 CTCGAG C^[CT]CG[GA]G
BbvCI 3213 GCTGAGG CCTCAGC(-5/-2)
BcgI 1792 CGAACACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3878 CTTAAG C^TTAAG
BlpI 3519 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3519 GCTGAGC GC^T[ACGT]AGC
BsaAI 3389 TACGTA [CT]AC^GT[GA]
BsaBI 3438 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2273 GAGACC GGTCTC(1/5)
BsiHKAI 1427 GAGCTC G[AT]GC[AT]^C
BsmBI 979 GAGACG CGTCTC(1/5)
BsoBI 3754 CTCGAG C^[CT]CG[GA]G
BsrBI 18 GAGCGG CCGCTC(-3/-3)
Bst1107I 4193 GTATAC GTA^TAC
BstAPI 2089 GCAACTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 75 CGCG CG^CG
BstZ17I 4193 GTATAC GTA^TAC
CelII 3519 GCTGAGC GC^T[ACGT]AGC
CfoI 2064 GCGC GCG^C
EcoRI 1369 GAATTC G^AATTC
EcoRV 3186 GATATC GAT^ATC
EcoT22I 3403 ATGCAT ATGCA^T
HaeII 2063 AGCGCC [GA]GCGC^[CT]
HhaI 2064 GCGC GCG^C
HinP1I 2064 GCGC G^CGC
KspI 74 CCGCGG CCGC^GG
MamI 3438 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 75 CGCG CG^CG
NcoI 244 CCATGG C^CATGG
NdeI 3169 CATATG CA^TATG
NsiI 3403 ATGCAT ATGCA^T
PaeR7I 3754 CTCGAG C^TCGAG
PflFI 3143 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 4313 AACGTT AA^CGTT
PvuII 3517 CAGCTG CAG^CTG
SacI 1427 GAGCTC GAGCT^C
SacII 74 CCGCGG CCGC^GG
SapI 3813 GCTCTTC GCTCTTC(1/4)
ScaI 1589 AGTACT AGT^ACT
SnaBI 3389 TACGTA TAC^GTA
SpeI 252 ACTAGT A^CTAGT
SphI 3154 GCATGC GCATG^C
Tth111I 3143 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 306 TCTAGA T^CTAGA
XhoI 3754 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 4193 GTATAC GT^[AC][GT]AC
4199 GTCTAC
AflIII 821 ACATGT A^C[GA][CT]GT
2866 ACATGT
BsgI 1515 GTGCAG GTGCAG(16/14)
1800 GTGCAG
BsmFI 7 GGGAC GGGAC(10/14)
96 GGGAC
BspHI 2243 TCATGA T^CATGA
3341 TCATGA
BspLU11I 821 ACATGT A^CATGT
2866 ACATGT
BspMI 1248 ACCTGC ACCTGC(4/8)
1802 GCAGGT
BsrDI 2196 CATTGC GCAATG(2/0)
2553 CATTGC
BstXI 625 CCAATGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2002 CCAGTTGTGTGG
DraI 1492 TTTAAA TTT^AAA
4755 TTTAAA
DsaI 74 CCGCGG C^C[GA][CT]GG
244 CCATGG
EcoNI 1417 CCTACTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1707 CCTGAAGGAGG
HincII 1637 GTCAAC GT[CT]^[GA]AC
2575 GTCAAC
HindII 1637 GTCAAC GT[CT]^[GA]AC
2575 GTCAAC
HindIII 1701 AAGCTT A^AGCTT
4654 AAGCTT
MfeI 523 CAATTG C^AATTG
2971 CAATTG
MslI 2538 CATTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4020 CATGAAGATG
MspA1I 74 CCGCGG C[AC]G^C[GT]G
3517 CAGCTG
MunI 523 CAATTG C^AATTG
2971 CAATTG
PshAI 521 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
868 GACTGCAGTC
RcaI 2243 TCATGA T^CATGA
3341 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AvaII BalI
BanII BbsI BbvI BciVI BclI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI