##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI Asp718I AspEI
AspHI AspI AvrII BamHI BbeI
BbrPI BcgI BfrI BglI BlnI
BsaAI BsaWI BseAI BsiHKAI BsiWI
BsmBI BspDI BspEI BspHI BsrDI
BssSI Bst1107I BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRV EcoT14I
FseI HpaI KasI KpnI MluI
MroI NarI NcoI NdeI NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SnaBI Sse8387I StyI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcyI 298 GACGCC G[GA]^CG[CT]C
AviII 2875 TGCGCA TGC^GCA
BalI 1104 TGGCCA TGG^CCA
BanI 552 GGTGCC G^G[CT][GA]CC
BbvCI 176 GCTGAGG CCTCAGC(-5/-2)
BciVI 1815 GGATAC GTATCC(6/5)
BsaBI 3407 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 298 GACGCC G[GA]^CG[CT]C
BsiEI 189 CGGCCG CG[GA][CT]^CG
Bsp1407I 844 TGTACA T^GTACA
BsrGI 844 TGTACA T^GTACA
BssHII 69 GCGCGC G^CGCGC
BstBI 2604 TTCGAA TT^CGAA
DraI 3409 TTTAAA TTT^AAA
DraIII 1347 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 189 CGGCCG C^GGCCG
EarI 3296 GAAGAG CTCTTC(1/4)
EclXI 189 CGGCCG C^GGCCG
Eco52I 189 CGGCCG C^GGCCG
EcoNI 2168 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2954 GAATTC G^AATTC
EcoT22I 2499 ATGCAT ATGCA^T
FspI 2875 TGCGCA TGC^GCA
Hin1I 298 GACGCC G[GA]^CG[CT]C
HincII 822 GTCAAC GT[CT]^[GA]AC
HindII 822 GTCAAC GT[CT]^[GA]AC
Ksp632I 3296 GAAGAG CTCTTC(1/4)
MamI 3407 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1104 TGGCCA TGG^CCA
MscI 1104 TGGCCA TGG^CCA
NsiI 2499 ATGCAT ATGCA^T
NspV 2604 TTCGAA TT^CGAA
PstI 2538 CTGCAG CTGCA^G
PvuII 2269 CAGCTG CAG^CTG
SfuI 2604 TTCGAA TT^CGAA
SmaI 508 CCCGGG CCC^GGG
SpeI 665 ACTAGT A^CTAGT
SphI 1888 GCATGC GCATG^C
SspBI 844 TGTACA T^GTACA
SspI 1839 AATATT AAT^ATT
StuI 1222 AGGCCT AGG^CCT
SwaI 3408 ATTTAAAT ATTT^AAAT
Tsp45I 2133 GTCAC ^GT[GC]AC
XbaI 2482 TCTAGA T^CTAGA
XmaCI 508 CCCGGG C^CCGGG
XmaI 508 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 758 AAATTT [GA]^AATT[CT]
2954 GAATTC
ApaI 95 GGGCCC GGGCC^C
194 GGGCCC
ApoI 758 AAATTT [GA]^AATT[CT]
2954 GAATTC
AseI 1130 ATTAAT AT^TAAT
3173 ATTAAT
AsnI 1130 ATTAAT AT^TAAT
3173 ATTAAT
BglII 2704 AGATCT A^GATCT
3249 AGATCT
BlpI 493 GCTCAGC GC^T[ACGT]AGC
2572 GCTCAGC
Bpu1102I 493 GCTCAGC GC^T[ACGT]AGC
2572 GCTCAGC
BsaI 2233 GAGACC GGTCTC(1/5)
3376 GAGACC
BsgI 687 GTGCAG GTGCAG(16/14)
1043 GTGCAG
BsmI 572 GAATGC GAATGC(1/-1)
1795 GAATGC
BsrBI 327 CCGCTC CCGCTC(-3/-3)
46 GAGCGG
BstAPI 400 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1729 GCAAACAATGC
BstXI 532 CCAGCAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2067 CCATACAGTTGG
BstYI 2704 AGATCT [GA]^GATC[CT]
3249 AGATCT
CelII 493 GCTCAGC GC^T[ACGT]AGC
2572 GCTCAGC
HaeII 173 GGCGCT [GA]GCGC^[CT]
486 AGCGCC
HgaI 298 GACGC GACGC(5/10)
1330 GCGTC
HindIII 954 AAGCTT A^AGCTT
3038 AAGCTT
KspI 129 CCGCGG CCGC^GG
216 CCGCGG
MaeII 1384 ACGT A^CGT
2914 ACGT
MflI 2704 AGATCT [GA]^GATC[CT]
3249 AGATCT
MslI 127 CACCGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2224 CACACCTGTG
PflMI 1401 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1457 CCAGCTCCTGG
PshBI 1130 ATTAAT AT^TAAT
3173 ATTAAT
SacII 129 CCGCGG CCGC^GG
216 CCGCGG
ScaI 2243 AGTACT AGT^ACT
2252 AGTACT
SmlI 1004 CTTGAG C^T[CT][GA]AG
2089 CTCAAG
Van91I 1401 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1457 CCAGCTCCTGG
XcmI 2666 CCAAATGAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2777 CCAAGTGGGGACTGG
XhoII 2704 AGATCT [GA]^GATC[CT]
3249 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI Asp700I AvaI AvaII
BanII BbsI BbvI BclI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspLU11I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MfeI MnlI MseI
MspA1I MspI MunI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XmnI