################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp718I AspEI AspHI AspI AvrII BamHI BbeI BbrPI BcgI BfrI BglI BlnI BsaAI BsaWI BseAI BsiHKAI BsiWI BsmBI BspDI BspEI BspHI BsrDI BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I ClaI CpoI DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRV EcoT14I FseI HpaI KasI KpnI MluI MroI NarI NcoI NdeI NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI SexAI SfiI SfoI SgrAI SnaBI Sse8387I StyI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcyI 298 GACGCC G[GA]^CG[CT]C AviII 2875 TGCGCA TGC^GCA BalI 1104 TGGCCA TGG^CCA BanI 552 GGTGCC G^G[CT][GA]CC BbvCI 176 GCTGAGG CCTCAGC(-5/-2) BciVI 1815 GGATAC GTATCC(6/5) BsaBI 3407 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 298 GACGCC G[GA]^CG[CT]C BsiEI 189 CGGCCG CG[GA][CT]^CG Bsp1407I 844 TGTACA T^GTACA BsrGI 844 TGTACA T^GTACA BssHII 69 GCGCGC G^CGCGC BstBI 2604 TTCGAA TT^CGAA DraI 3409 TTTAAA TTT^AAA DraIII 1347 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 189 CGGCCG C^GGCCG EarI 3296 GAAGAG CTCTTC(1/4) EclXI 189 CGGCCG C^GGCCG Eco52I 189 CGGCCG C^GGCCG EcoNI 2168 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2954 GAATTC G^AATTC EcoT22I 2499 ATGCAT ATGCA^T FspI 2875 TGCGCA TGC^GCA Hin1I 298 GACGCC G[GA]^CG[CT]C HincII 822 GTCAAC GT[CT]^[GA]AC HindII 822 GTCAAC GT[CT]^[GA]AC Ksp632I 3296 GAAGAG CTCTTC(1/4) MamI 3407 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1104 TGGCCA TGG^CCA MscI 1104 TGGCCA TGG^CCA NsiI 2499 ATGCAT ATGCA^T NspV 2604 TTCGAA TT^CGAA PstI 2538 CTGCAG CTGCA^G PvuII 2269 CAGCTG CAG^CTG SfuI 2604 TTCGAA TT^CGAA SmaI 508 CCCGGG CCC^GGG SpeI 665 ACTAGT A^CTAGT SphI 1888 GCATGC GCATG^C SspBI 844 TGTACA T^GTACA SspI 1839 AATATT AAT^ATT StuI 1222 AGGCCT AGG^CCT SwaI 3408 ATTTAAAT ATTT^AAAT Tsp45I 2133 GTCAC ^GT[GC]AC XbaI 2482 TCTAGA T^CTAGA XmaCI 508 CCCGGG C^CCGGG XmaI 508 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcsI 758 AAATTT [GA]^AATT[CT] 2954 GAATTC ApaI 95 GGGCCC GGGCC^C 194 GGGCCC ApoI 758 AAATTT [GA]^AATT[CT] 2954 GAATTC AseI 1130 ATTAAT AT^TAAT 3173 ATTAAT AsnI 1130 ATTAAT AT^TAAT 3173 ATTAAT BglII 2704 AGATCT A^GATCT 3249 AGATCT BlpI 493 GCTCAGC GC^T[ACGT]AGC 2572 GCTCAGC Bpu1102I 493 GCTCAGC GC^T[ACGT]AGC 2572 GCTCAGC BsaI 2233 GAGACC GGTCTC(1/5) 3376 GAGACC BsgI 687 GTGCAG GTGCAG(16/14) 1043 GTGCAG BsmI 572 GAATGC GAATGC(1/-1) 1795 GAATGC BsrBI 327 CCGCTC CCGCTC(-3/-3) 46 GAGCGG BstAPI 400 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1729 GCAAACAATGC BstXI 532 CCAGCAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2067 CCATACAGTTGG BstYI 2704 AGATCT [GA]^GATC[CT] 3249 AGATCT CelII 493 GCTCAGC GC^T[ACGT]AGC 2572 GCTCAGC HaeII 173 GGCGCT [GA]GCGC^[CT] 486 AGCGCC HgaI 298 GACGC GACGC(5/10) 1330 GCGTC HindIII 954 AAGCTT A^AGCTT 3038 AAGCTT KspI 129 CCGCGG CCGC^GG 216 CCGCGG MaeII 1384 ACGT A^CGT 2914 ACGT MflI 2704 AGATCT [GA]^GATC[CT] 3249 AGATCT MslI 127 CACCGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2224 CACACCTGTG PflMI 1401 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1457 CCAGCTCCTGG PshBI 1130 ATTAAT AT^TAAT 3173 ATTAAT SacII 129 CCGCGG CCGC^GG 216 CCGCGG ScaI 2243 AGTACT AGT^ACT 2252 AGTACT SmlI 1004 CTTGAG C^T[CT][GA]AG 2089 CTCAAG Van91I 1401 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1457 CCAGCTCCTGG XcmI 2666 CCAAATGAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2777 CCAAGTGGGGACTGG XhoII 2704 AGATCT [GA]^GATC[CT] 3249 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI Asp700I AvaI AvaII BanII BbsI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MfeI MnlI MseI MspA1I MspI MunI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XmnI