##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AclI AhdI Alw44I
AocI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BamHI BcgI BclI BsiWI BsmBI
Bsp1407I BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII KpnI KspI
MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 2659 TCCGGA T^CCGGA
AflIII 4178 ACACGT A^C[GA][CT]GT
AgeI 256 ACCGGT A^CCGGT
Aor51HI 2680 AGCGCT AGC^GCT
ApaI 590 GGGCCC GGGCC^C
BalI 4249 TGGCCA TGG^CCA
BbeI 1024 GGCGCC GGCGC^C
BbsI 1867 GTCTTC GAAGAC(2/6)
BglI 2179 GCCAATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 503 AGATCT A^GATCT
BpuAI 1867 GTCTTC GAAGAC(2/6)
BseAI 2659 TCCGGA T^CCGGA
BsiEI 738 CGACCG CG[GA][CT]^CG
BsmI 1239 GAATGC GAATGC(1/-1)
BspDI 2949 ATCGAT AT^CGAT
BspEI 2659 TCCGGA T^CCGGA
BsrFI 256 ACCGGT [GA]^CCGG[CT]
Cfr10I 256 ACCGGT [GA]^CCGG[CT]
ClaI 2949 ATCGAT AT^CGAT
Eco47III 2680 AGCGCT AGC^GCT
EcoNI 4158 CCTATCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 1024 GGCGCC G^GCGCC
MfeI 4737 CAATTG C^AATTG
MluNI 4249 TGGCCA TGG^CCA
MroI 2659 TCCGGA T^CCGGA
MscI 4249 TGGCCA TGG^CCA
MunI 4737 CAATTG C^AATTG
NarI 1024 GGCGCC GG^CGCC
PaeR7I 1109 CTCGAG C^TCGAG
PinAI 256 ACCGGT A^CCGGT
PshAI 801 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1301 GAGCTC GAGCT^C
SfoI 1024 GGCGCC GGC^GCC
SnaBI 4435 TACGTA TAC^GTA
XhoI 1109 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 721 GTCTAC GT^[AC][GT]AC
4018 GTAGAC
AccII 270 CGCG CG^CG
4927 CGCG
AflII 3651 CTTAAG C^TTAAG
4351 CTTAAG
BanI 280 GGTGCC G^G[CT][GA]CC
1024 GGCGCC
BbrPI 1384 CACGTG CAC^GTG
1630 CACGTG
BbvCI 2691 CCTCAGC CCTCAGC(-5/-2)
1186 GCTGAGG
BciVI 771 GGATAC GTATCC(6/5)
835 GGATAC
BfrI 3651 CTTAAG C^TTAAG
4351 CTTAAG
BlpI 482 GCTTAGC GC^T[ACGT]AGC
4061 GCTAAGC
Bpu1102I 482 GCTTAGC GC^T[ACGT]AGC
4061 GCTAAGC
BsaBI 1287 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1824 GATCTCCATC
BspHI 1832 TCATGA T^CATGA
4949 TCATGA
BsrBI 874 GAGCGG CCGCTC(-3/-3)
5199 GAGCGG
BstAPI 1308 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1615 GCAGAGATTGC
BstUI 270 CGCG CG^CG
4927 CGCG
CelII 482 GCTTAGC GC^T[ACGT]AGC
4061 GCTAAGC
FauI 2864 CCCGC CCCGC(4/6)
2873 GCGGG
HaeII 1024 GGCGCC [GA]GCGC^[CT]
2680 AGCGCT
HpaI 33 GTTAAC GTT^AAC
5325 GTTAAC
MamI 1287 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1824 GATCTCCATC
MvnI 270 CGCG CG^CG
4927 CGCG
PflMI 3575 CCACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4670 CCATATTCTGG
PmaCI 1384 CACGTG CAC^GTG
1630 CACGTG
PmlI 1384 CACGTG CAC^GTG
1630 CACGTG
RcaI 1832 TCATGA T^CATGA
4949 TCATGA
SapI 1736 GAAGAGC GCTCTTC(1/4)
3788 GAAGAGC
StuI 492 AGGCCT AGG^CCT
1700 AGGCCT
Van91I 3575 CCACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4670 CCATATTCTGG
XcmI 1264 CCAGCTGTTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3587 CCAAACCTCTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII AvrII BanII BbvI BcnI
BfaI BlnI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII