##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII BbrPI BcgI BfrI BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FspI HindIII HpaI KpnI MamI
MfeI MluI MroI MunI NdeI
NotI NruI NsiI PacI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RsrII SacI ScaI SfiI SspBI
SspI SwaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AgeI 2584 ACCGGT A^CCGGT
Asp700I 2042 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3627 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 4255 TGGCCA TGG^CCA
BbeI 80 GGCGCC GGCGC^C
BclI 1180 TGATCA T^GATCA
BglII 3903 AGATCT A^GATCT
BsaAI 307 TACGTA [CT]AC^GT[GA]
BsaI 3487 GAGACC GGTCTC(1/5)
BsaWI 2584 ACCGGT [AT]^CCGG[AT]
BsiEI 241 CGATCG CG[GA][CT]^CG
BsmI 3442 GAATGC GAATGC(1/-1)
BspHI 919 TCATGA T^CATGA
BssSI 3750 CACGAG CACGAG(-5/-1)
BstBI 1985 TTCGAA TT^CGAA
DrdI 452 GACCCCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1180 TGATCA T^GATCA
FseI 2360 GGCCGGCC GGCCGG^CC
KasI 80 GGCGCC G^GCGCC
MluNI 4255 TGGCCA TGG^CCA
MscI 4255 TGGCCA TGG^CCA
MseI 4987 TTAA T^TAA
NarI 80 GGCGCC GG^CGCC
NheI 4490 GCTAGC G^CTAGC
NspI 1665 GCATGC [GA]CATG^[CT]
NspV 1985 TTCGAA TT^CGAA
PflFI 3627 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2584 ACCGGT A^CCGGT
PvuI 241 CGATCG CGAT^CG
PvuII 4322 CAGCTG CAG^CTG
RcaI 919 TCATGA T^CATGA
SalI 4679 GTCGAC G^TCGAC
SexAI 3628 ACCTGGT A^CC[AT]GGT
SfoI 80 GGCGCC GGC^GCC
SfuI 1985 TTCGAA TT^CGAA
SgrAI 2583 CACCGGTG C[GA]^CCGG[CT]G
SnaBI 307 TACGTA TAC^GTA
SphI 1665 GCATGC GCATG^C
Tru9I 4987 TTAA T^TAA
Tth111I 3627 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2455 TCTAGA T^CTAGA
XmnI 2042 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 4313 GTAGAC GT^[AC][GT]AC
4679 GTCGAC
AcsI 740 GAATTT [GA]^AATT[CT]
841 AAATTC
AcyI 80 GGCGCC G[GA]^CG[CT]C
580 GACGCC
ApoI 740 GAATTT [GA]^AATT[CT]
841 AAATTC
AspHI 684 GAGCAC G[AT]GC[AT]^C
2352 GTGCTC
BamHI 151 GGATCC G^GATCC
2590 GGATCC
BsaHI 80 GGCGCC G[GA]^CG[CT]C
580 GACGCC
BsiHKAI 684 GAGCAC G[AT]GC[AT]^C
2352 GTGCTC
BsmBI 4628 CGTCTC CGTCTC(1/5)
59 GAGACG
Hin1I 80 GGCGCC G[GA]^CG[CT]C
580 GACGCC
KspI 42 CCGCGG CCGC^GG
973 CCGCGG
PaeR7I 732 CTCGAG C^TCGAG
4758 CTCGAG
SacII 42 CCGCGG CCGC^GG
973 CCGCGG
SapI 1673 GAAGAGC GCTCTTC(1/4)
1874 GAAGAGC
SbfI 198 CCTGCAGG CCTGCA^GG
751 CCTGCAGG
SpeI 466 ACTAGT A^CTAGT
3653 ACTAGT
Sse8387I 198 CCTGCAGG CCTGCA^GG
751 CCTGCAGG
StuI 3419 AGGCCT AGG^CCT
4695 AGGCCT
XhoI 732 CTCGAG C^TCGAG
4758 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AvaI AvaII
AvrII BanI BanII BbsI BbvCI
BbvI BciVI BcnI BfaI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI