##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I ApaLI AscI AspEI AviII
BamHI BbeI BbrPI BclI BfrI
BlpI Bpu1102I BsiEI BsiWI BsmBI
BspDI BspLU11I BssHII Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoRV EcoT22I FbaI FspI HpaI
KasI MluI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII ScaI SfoI
SfuI SgrAI SnaBI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 1587 GGTACC G^GTACC
AccIII 4745 TCCGGA T^CCGGA
AflIII 5265 ACGTGT A^C[GA][CT]GT
Aor51HI 17 AGCGCT AGC^GCT
AseI 3298 ATTAAT AT^TAAT
AsnI 3298 ATTAAT AT^TAAT
Asp700I 779 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1587 GGTACC G^GTACC
AspI 4208 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 3069 GAAGAC GAAGAC(2/6)
BcgI 1972 GCAGAAACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3828 GGATAC GTATCC(6/5)
BpuAI 3069 GAAGAC GAAGAC(2/6)
BsaAI 4470 CACGTA [CT]AC^GT[GA]
BsaBI 4329 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 789 GAGACC GGTCTC(1/5)
BseAI 4745 TCCGGA T^CCGGA
Bsp1407I 5273 TGTACA T^GTACA
BspEI 4745 TCCGGA T^CCGGA
BspHI 2265 TCATGA T^CATGA
BsrDI 2417 GCAATG GCAATG(2/0)
BsrGI 5273 TGTACA T^GTACA
BstEII 451 GGTGACC G^GT[ACGT]ACC
BstPI 451 GGTGACC G^GT[ACGT]ACC
Eco47III 17 AGCGCT AGC^GCT
EcoO65I 451 GGTGACC G^GT[ACGT]ACC
FseI 237 GGCCGGCC GGCCGG^CC
HindIII 4183 AAGCTT A^AGCTT
KpnI 1587 GGTACC GGTAC^C
KspI 79 CCGCGG CCGC^GG
MamI 4329 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3442 CAATTG C^AATTG
MroI 4745 TCCGGA T^CCGGA
MunI 3442 CAATTG C^AATTG
PflFI 4208 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 844 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3298 ATTAAT AT^TAAT
RcaI 2265 TCATGA T^CATGA
SacII 79 CCGCGG CCGC^GG
SalI 3525 GTCGAC G^TCGAC
SfiI 346 GGCCAGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 3801 ACTAGT A^CTAGT
SphI 204 GCATGC GCATG^C
SspBI 5273 TGTACA T^GTACA
Tth111I 4208 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 779 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 851 GTCTAC GT^[AC][GT]AC
3525 GTCGAC
ApaI 1597 GGGCCC GGGCC^C
4583 GGGCCC
AvrII 193 CCTAGG C^CTAGG
3821 CCTAGG
BbvCI 385 CCTCAGC CCTCAGC(-5/-2)
2627 CCTCAGC
BlnI 193 CCTAGG C^CTAGG
3821 CCTAGG
BsaWI 132 ACCGGA [AT]^CCGG[AT]
4745 TCCGGA
BsmI 691 GAATGC GAATGC(1/-1)
1324 GAATGC
DraIII 4382 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4688 CACTCAGTG
EcoRI 1472 GAATTC G^AATTC
2501 GAATTC
HaeII 17 AGCGCT [GA]GCGC^[CT]
69 GGCGCT
HincII 3525 GTCGAC GT[CT]^[GA]AC
3779 GTTGAC
HindII 3525 GTCGAC GT[CT]^[GA]AC
3779 GTTGAC
NaeI 238 GCCGGC GCC^GGC
2327 GCCGGC
NgoMIV 238 GCCGGC G^CCGGC
2327 GCCGGC
SbfI 1708 CCTGCAGG CCTGCA^GG
3423 CCTGCAGG
SexAI 744 ACCTGGT A^CC[AT]GGT
4383 ACCAGGT
Sse8387I 1708 CCTGCAGG CCTGCA^GG
3423 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BglI
BglII BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI