##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AclI AcsI AflII AocI ApoI
AseI AsnI Asp700I AviII BciVI
BclI BfrI BsiWI BsmBI Bsp1407I
BspHI BsrGI Bst1107I BstBI BstZ17I
Bsu36I CpoI DraI DrdI Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI MfeI MluI
MunI NdeI NotI NruI NsiI
NspV PacI PmeI PshBI Psp1406I
PvuI RcaI RsrII SapI SbfI
ScaI SexAI SfuI SnaBI Sse8387I
SspBI SspI SwaI Tsp509I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 4460 GGTACC G^GTACC
AccI 1005 GTCGAC GT^[AC][GT]AC
AccIII 1556 TCCGGA T^CCGGA
AhdI 4292 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 24 GGCGCGCC GG^CGCGCC
Asp718I 4460 GGTACC G^GTACC
AspEI 4292 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4473 CCTAGG C^CTAGG
BamHI 69 GGATCC G^GATCC
BbsI 2187 GAAGAC GAAGAC(2/6)
BbvCI 4159 GCTGAGG CCTCAGC(-5/-2)
BglII 2239 AGATCT A^GATCT
BlnI 4473 CCTAGG C^CTAGG
BlpI 3826 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3826 GCTCAGC GC^T[ACGT]AGC
BpuAI 2187 GAAGAC GAAGAC(2/6)
BsaBI 872 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2280 GAGACC GGTCTC(1/5)
BseAI 1556 TCCGGA T^CCGGA
BsmI 378 GAATGC GAATGC(1/-1)
BspDI 999 ATCGAT AT^CGAT
BspEI 1556 TCCGGA T^CCGGA
BspLU11I 954 ACATGT A^CATGT
BssSI 1221 CACGAG CACGAG(-5/-1)
CelII 3826 GCTCAGC GC^T[ACGT]AGC
ClaI 999 ATCGAT AT^CGAT
DraIII 3744 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 4292 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 1005 GTCGAC GT[CT]^[GA]AC
HindII 1005 GTCGAC GT[CT]^[GA]AC
KpnI 4460 GGTACC GGTAC^C
MamI 872 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1556 TCCGGA T^CCGGA
NheI 713 GCTAGC G^CTAGC
PaeR7I 2108 CTCGAG C^TCGAG
PflMI 197 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2967 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1005 GTCGAC G^TCGAC
SpeI 3889 ACTAGT A^CTAGT
TfiI 4414 GATTC G^A[AT]TC
Van91I 197 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2108 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AatII 1353 GACGTC GACGT^C
2868 GACGTC
AgeI 409 ACCGGT A^CCGGT
3382 ACCGGT
Alw44I 950 GTGCAC G^TGCAC
1043 GTGCAC
ApaLI 950 GTGCAC G^TGCAC
1043 GTGCAC
BstEII 354 GGTGACC G^GT[ACGT]ACC
3953 GGTGACC
BstPI 354 GGTGACC G^GT[ACGT]ACC
3953 GGTGACC
BstYI 69 GGATCC [GA]^GATC[CT]
2239 AGATCT
EcoO65I 354 GGTGACC G^GT[ACGT]ACC
3953 GGTGACC
MflI 69 GGATCC [GA]^GATC[CT]
2239 AGATCT
MseI 7 TTAA T^TAA
496 TTAA
PinAI 409 ACCGGT A^CCGGT
3382 ACCGGT
SacI 2111 GAGCTC GAGCT^C
3986 GAGCTC
SmlI 2108 CTCGAG C^T[CT][GA]AG
4071 CTTGAG
Tru9I 7 TTAA T^TAA
496 TTAA
XhoII 69 GGATCC [GA]^GATC[CT]
2239 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI Aor51HI ApaI
AspHI AspI AvaI AvaII BalI
BanI BanII BbeI BbrPI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BsaAI BsaHI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssHII BssKI BstAPI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SfoI SgrAI SmaI SphI StuI
StyI TaqI TseI Tsp45I TspRI
Tth111I TthHB8I XcmI XmaCI XmaI