##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I BbrPI BclI
BfrI BglII BsaBI BseAI BsiWI
BspEI BspHI Bst1107I BstZ17I CpoI
DraI Eco47III EcoRV FbaI HindIII
HpaI KpnI MamI MfeI MroI
MunI NdeI NheI NotI NruI
PacI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SapI SgrAI SnaBI SpeI SspI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 747 GTCGAC GT^[AC][GT]AC
AgeI 638 ACCGGT A^CCGGT
AhdI 3285 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3285 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 572 TGCGCA TGC^GCA
AvrII 4258 CCTAGG C^CTAGG
BcgI 2654 GCAGCGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3014 GGATAC GTATCC(6/5)
BlnI 4258 CCTAGG C^CTAGG
BsaI 823 GAGACC GGTCTC(1/5)
BsmBI 4603 GAGACG CGTCTC(1/5)
BspDI 1856 ATCGAT AT^CGAT
BspLU11I 4583 ACATGT A^CATGT
BsrDI 4846 GCAATG GCAATG(2/0)
BssHII 1194 GCGCGC G^CGCGC
BstBI 4764 TTCGAA TT^CGAA
ClaI 1856 ATCGAT AT^CGAT
DraIII 3560 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3285 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1390 CTCTTC CTCTTC(1/4)
EcoT22I 4790 ATGCAT ATGCA^T
FseI 9 GGCCGGCC GGCCGG^CC
FspI 572 TGCGCA TGC^GCA
HincII 747 GTCGAC GT[CT]^[GA]AC
HindII 747 GTCGAC GT[CT]^[GA]AC
Ksp632I 1390 CTCTTC CTCTTC(1/4)
MluI 4606 ACGCGT A^CGCGT
NsiI 4790 ATGCAT ATGCA^T
NspV 4764 TTCGAA TT^CGAA
PaeR7I 2120 CTCGAG C^TCGAG
PflMI 3556 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 638 ACCGGT A^CCGGT
SalI 747 GTCGAC G^TCGAC
SbfI 2333 CCTGCAGG CCTGCA^GG
SfuI 4764 TTCGAA TT^CGAA
SphI 4273 GCATGC GCATG^C
Sse8387I 2333 CCTGCAGG CCTGCA^GG
Van91I 3556 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2120 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AspI 871 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
970 GACCAGGTC
BsaAI 829 TACGTG [CT]AC^GT[GA]
3803 TACGTG
BsmI 3612 GAATGC GAATGC(1/-1)
225 GCATTC
BssSI 2038 CTCGTG CACGAG(-5/-1)
2524 CTCGTG
BstAPI 2845 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4783 GCAGGAGATGC
BstEII 4184 GGTCACC G^GT[ACGT]ACC
4655 GGTTACC
BstPI 4184 GGTCACC G^GT[ACGT]ACC
4655 GGTTACC
BstXI 1665 CCAGGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2373 CCACCTCACTGG
DrdI 967 GACGACCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1782 GACTACAGGGTC
EcoO65I 4184 GGTCACC G^GT[ACGT]ACC
4655 GGTTACC
EcoRI 1873 GAATTC G^AATTC
4410 GAATTC
PflFI 871 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
970 GACCAGGTC
SacI 246 GAGCTC GAGCT^C
3222 GAGCTC
ScaI 647 AGTACT AGT^ACT
2933 AGTACT
SfiI 3431 GGCCAGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3768 GGCCTCCCTGGCC
TfiI 2634 GAATC G^A[AT]TC
3299 GATTC
Tth111I 871 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
970 GACCAGGTC
XcmI 254 CCATCAACGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
326 CCACACCCACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BamHI BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SfoI SmaI SmlI
SspBI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmaCI XmaI