##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AclI AgeI Alw44I ApaLI
AscI AseI AsnI Asp718I AvrII
BciVI BclI BlnI BssHII BstBI
BstEII BstPI CpoI DraIII DrdI
EcoO65I EcoRV EcoT22I FbaI FseI
HindIII HpaI KpnI MluI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 2323 TCCGGA T^CCGGA
AflII 28 CTTAAG C^TTAAG
ApaI 4868 GGGCCC GGGCC^C
AviII 638 TGCGCA TGC^GCA
BfrI 28 CTTAAG C^TTAAG
BlpI 4267 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4267 GCTGAGC GC^T[ACGT]AGC
BsaBI 4093 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2323 TCCGGA [AT]^CCGG[AT]
BseAI 2323 TCCGGA T^CCGGA
BsiWI 2591 CGTACG C^GTACG
BsmI 3201 GCATTC GAATGC(1/-1)
Bsp1407I 685 TGTACA T^GTACA
BspEI 2323 TCCGGA T^CCGGA
BspHI 4603 TCATGA T^CATGA
BspLU11I 4366 ACATGT A^CATGT
BsrDI 1157 CATTGC GCAATG(2/0)
BsrGI 685 TGTACA T^GTACA
BssSI 1698 CTCGTG CACGAG(-5/-1)
Bst1107I 405 GTATAC GTA^TAC
BstZ17I 405 GTATAC GTA^TAC
CelII 4267 GCTGAGC GC^T[ACGT]AGC
EcoRI 4427 GAATTC G^AATTC
FspI 638 TGCGCA TGC^GCA
MamI 4093 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 429 CAATTG C^AATTG
MroI 2323 TCCGGA T^CCGGA
MunI 429 CAATTG C^AATTG
NcoI 2186 CCATGG C^CATGG
PaeR7I 622 CTCGAG C^TCGAG
RcaI 4603 TCATGA T^CATGA
SapI 2511 GAAGAGC GCTCTTC(1/4)
SexAI 960 ACCAGGT A^CC[AT]GGT
SphI 3090 GCATGC GCATG^C
SspBI 685 TGTACA T^GTACA
StuI 4781 AGGCCT AGG^CCT
XhoI 622 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 2485 GACGTC GACGT^C
4252 GACGTC
AccI 405 GTATAC GT^[AC][GT]AC
3914 GTCTAC
AflIII 128 ACACGT A^C[GA][CT]GT
4366 ACATGT
Aor51HI 4564 AGCGCT AGC^GCT
4644 AGCGCT
BamHI 657 GGATCC G^GATCC
4230 GGATCC
BbeI 1315 GGCGCC GGCGC^C
2465 GGCGCC
BbrPI 1611 CACGTG CAC^GTG
4715 CACGTG
BfaI 775 CTAG C^TAG
3095 CTAG
BglI 285 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4272 GCCACGCCGGC
BglII 24 AGATCT A^GATCT
4092 AGATCT
BstAPI 947 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1544 GCAGCCTCTGC
DraI 2064 TTTAAA TTT^AAA
2161 TTTAAA
Eco47III 4564 AGCGCT AGC^GCT
4644 AGCGCT
EcoNI 2901 CCTTGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4773 CCTGAGGGAGG
HincII 166 GTCAAC GT[CT]^[GA]AC
1894 GTCAAC
HindII 166 GTCAAC GT[CT]^[GA]AC
1894 GTCAAC
KasI 1315 GGCGCC G^GCGCC
2465 GGCGCC
KspI 3439 CCGCGG CCGC^GG
4864 CCGCGG
MaeI 775 CTAG C^TAG
3095 CTAG
NaeI 1466 GCCGGC GCC^GGC
4277 GCCGGC
NarI 1315 GGCGCC GG^CGCC
2465 GGCGCC
NgoMIV 1466 GCCGGC G^CCGGC
4277 GCCGGC
PmaCI 1611 CACGTG CAC^GTG
4715 CACGTG
PmlI 1611 CACGTG CAC^GTG
4715 CACGTG
PstI 245 CTGCAG CTGCA^G
1542 CTGCAG
SacI 1687 GAGCTC GAGCT^C
4506 GAGCTC
SacII 3439 CCGCGG CCGC^GG
4864 CCGCGG
SfoI 1315 GGCGCC GGC^GCC
2465 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI AlwNI AocI
ApoI Asp700I AspEI AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspDI BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I ClaI
DdeI DpnII DraII DsaI EaeI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI