##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AspEI AvaI AviII BamHI
BbeI BbrPI BcgI BglI BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsoBI BspDI BspEI BsrBI BssHII
BstEII BstPI BstUI ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I FseI FspI HgaI Hin1I
HpaI KasI KspI MluI MroI
MvnI NaeI NarI NgoMIV NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI PvuI RsrII SacII
SalI SbfI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 3327 GGTACC G^GTACC
AclI 1602 AACGTT AA^CGTT
Asp718I 3327 GGTACC G^GTACC
AvrII 7820 CCTAGG C^CTAGG
BalI 6552 TGGCCA TGG^CCA
BclI 6448 TGATCA T^GATCA
BglII 2962 AGATCT A^GATCT
BlnI 7820 CCTAGG C^CTAGG
Bsp1407I 6613 TGTACA T^GTACA
BspHI 6360 TCATGA T^CATGA
BspLU11I 3368 ACATGT A^CATGT
BspMI 1703 ACCTGC ACCTGC(4/8)
BsrFI 7574 ACCGGC [GA]^CCGG[CT]
BsrGI 6613 TGTACA T^GTACA
BssSI 2446 CACGAG CACGAG(-5/-1)
Cfr10I 7574 ACCGGC [GA]^CCGG[CT]
DraIII 5757 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 6952 CCTACAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 4277 GCGGG CCCGC(4/6)
FbaI 6448 TGATCA T^GATCA
HincII 5786 GTCAAC GT[CT]^[GA]AC
HindII 5786 GTCAAC GT[CT]^[GA]AC
KpnI 3327 GGTACC GGTAC^C
MfeI 4521 CAATTG C^AATTG
MluNI 6552 TGGCCA TGG^CCA
MscI 6552 TGGCCA TGG^CCA
MunI 4521 CAATTG C^AATTG
NheI 7537 GCTAGC G^CTAGC
NspI 3368 ACATGT [GA]CATG^[CT]
PshAI 3690 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1602 AACGTT AA^CGTT
RcaI 6360 TCATGA T^CATGA
SacI 3901 GAGCTC GAGCT^C
SexAI 6734 ACCAGGT A^CC[AT]GGT
SspBI 6613 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 3368 ACATGT A^C[GA][CT]GT
6307 ACACGT
AocI 7314 CCTCAGG CC^T[ACGT]AGG
7917 CCTGAGG
AseI 1466 ATTAAT AT^TAAT
1586 ATTAAT
AsnI 1466 ATTAAT AT^TAAT
1586 ATTAAT
BbvCI 4606 CCTCAGC CCTCAGC(-5/-2)
53 GCTGAGG
BlpI 5420 GCTAAGC GC^T[ACGT]AGC
7116 GCTTAGC
Bpu1102I 5420 GCTAAGC GC^T[ACGT]AGC
7116 GCTTAGC
BsmBI 6686 CGTCTC CGTCTC(1/5)
6804 CGTCTC
BsrDI 1794 CATTGC GCAATG(2/0)
6313 CATTGC
Bst1107I 6199 GTATAC GTA^TAC
6434 GTATAC
BstAPI 4670 GCAGCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6012 GCAAATTATGC
BstBI 1461 TTCGAA TT^CGAA
2760 TTCGAA
BstZ17I 6199 GTATAC GTA^TAC
6434 GTATAC
Bsu36I 7314 CCTCAGG CC^T[ACGT]AGG
7917 CCTGAGG
CelII 5420 GCTAAGC GC^T[ACGT]AGC
7116 GCTTAGC
EaeI 6552 TGGCCA [CT]^GGCC[GA]
7492 TGGCCG
Eco81I 7314 CCTCAGG CC^T[ACGT]AGG
7917 CCTGAGG
HaeII 1125 AGCGCC [GA]GCGC^[CT]
2122 AGCGCC
NdeI 1937 CATATG CA^TATG
7695 CATATG
NspV 1461 TTCGAA TT^CGAA
2760 TTCGAA
PshBI 1466 ATTAAT AT^TAAT
1586 ATTAAT
SapI 1958 GCTCTTC GCTCTTC(1/4)
3161 GCTCTTC
SfuI 1461 TTCGAA TT^CGAA
2760 TTCGAA
SpeI 5587 ACTAGT A^CTAGT
6860 ACTAGT
XbaI 6262 TCTAGA T^CTAGA
6289 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI AlwNI ApoI Asp700I
AspHI AspI AvaII BanI BanII
BbsI BbvI BciVI BcnI BfaI
BfrI BmyI BpmI BpuAI BsaBI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DrdI
DsaI EarI EcoO109I EcoRI EcoRII
EcoRV EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmnI