##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BbeI BbrPI BbvCI
BcgI BciVI BlnI BsaHI BsaWI
BseAI BsiWI BspDI BspEI BssHII
BssSI BstAPI BstBI BstEII BstPI
ClaI CpoI Eam1105I Eco47III EcoO65I
EcoT22I FseI FspI HaeII Hin1I
HpaI KasI KpnI KspI MluI
MroI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SphI
Sse8387I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AflII 2915 CTTAAG C^TTAAG
AocI 1659 CCTGAGG CC^T[ACGT]AGG
AvaI 1331 CTCGGG C^[CT]CG[GA]G
BamHI 198 GGATCC G^GATCC
BbsI 4423 GTCTTC GAAGAC(2/6)
BclI 2272 TGATCA T^GATCA
BfrI 2915 CTTAAG C^TTAAG
BglI 2837 GCCATTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 443 AGATCT A^GATCT
BlpI 3114 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3114 GCTGAGC GC^T[ACGT]AGC
BpuAI 4423 GTCTTC GAAGAC(2/6)
BsaBI 1186 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1635 CGGCCG CG[GA][CT]^CG
BsmBI 33 GAGACG CGTCTC(1/5)
BsoBI 1331 CTCGGG C^[CT]CG[GA]G
BsrBI 23 GAGCGG CCGCTC(-3/-3)
BsrFI 1633 GCCGGC [GA]^CCGG[CT]
Bst1107I 2204 GTATAC GTA^TAC
BstXI 4306 CCACTGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2204 GTATAC GTA^TAC
Bsu36I 1659 CCTGAGG CC^T[ACGT]AGG
CelII 3114 GCTGAGC GC^T[ACGT]AGC
CfoI 98 GCGC GCG^C
Cfr10I 1633 GCCGGC [GA]^CCGG[CT]
EagI 1635 CGGCCG C^GGCCG
EclXI 1635 CGGCCG C^GGCCG
Eco52I 1635 CGGCCG C^GGCCG
Eco81I 1659 CCTGAGG CC^T[ACGT]AGG
EcoNI 4550 CCTCTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3798 GAATTC G^AATTC
EcoRV 1186 GATATC GAT^ATC
EcoT14I 1409 CCTTGG C^C[AT][AT]GG
FbaI 2272 TGATCA T^GATCA
HhaI 98 GCGC GCG^C
HinP1I 98 GCGC G^CGC
HincII 3000 GTCAAC GT[CT]^[GA]AC
HindII 3000 GTCAAC GT[CT]^[GA]AC
MamI 1186 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1633 GCCGGC GCC^GGC
NgoMIV 1633 GCCGGC G^CCGGC
SacI 505 GAGCTC GAGCT^C
SnaBI 4765 TACGTA TAC^GTA
SpeI 3292 ACTAGT A^CTAGT
StyI 1409 CCTTGG C^C[AT][AT]GG
SwaI 2014 ATTTAAAT ATTT^AAAT
XbaI 930 TCTAGA T^CTAGA
XcmI 2677 CCAGGGGCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 69 ACATGT A^C[GA][CT]GT
2719 ACATGT
AlwNI 3130 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
4152 CAGCTACTG
ApaI 2681 GGGCCC GGGCC^C
4607 GGGCCC
AspI 1836 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3227 GACAGGGTC
BalI 4315 TGGCCA TGG^CCA
4975 TGGCCA
BcnI 453 CCGGG CC^[GC]GG
1638 CCGGG
BsaAI 3555 TACGTG [CT]AC^GT[GA]
4765 TACGTA
BseRI 319 GAGGAG GAGGAG(10/8)
4921 GAGGAG
Bsp1407I 104 TGTACA T^GTACA
1364 TGTACA
BspHI 912 TCATGA T^CATGA
4012 TCATGA
BspLU11I 69 ACATGT A^CATGT
2719 ACATGT
BsrGI 104 TGTACA T^GTACA
1364 TGTACA
DraIII 1309 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3513 CACCTGGTG
DrdI 35 GACGTGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3823 GACCCAGGGGTC
DsaI 497 CCGTGG C^C[GA][CT]GG
4428 CCACGG
MfeI 3438 CAATTG C^AATTG
4890 CAATTG
MluNI 4315 TGGCCA TGG^CCA
4975 TGGCCA
MscI 4315 TGGCCA TGG^CCA
4975 TGGCCA
MunI 3438 CAATTG C^AATTG
4890 CAATTG
NciI 453 CCGGG CC^[GC]GG
1638 CCGGG
PflFI 1836 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3227 GACAGGGTC
PpuMI 355 GGGACCT [GA]G^G[AT]CC[CT]
4367 GGGACCT
RcaI 912 TCATGA T^CATGA
4012 TCATGA
SapI 781 GAAGAGC GCTCTTC(1/4)
208 GCTCTTC
SexAI 1171 ACCTGGT A^CC[AT]GGT
3514 ACCTGGT
SspBI 104 TGTACA T^GTACA
1364 TGTACA
Tth111I 1836 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3227 GACAGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI Asp700I AspHI
AvaII BanI BanII BbvI BfaI
BmyI BpmI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StuI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI