##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AflII AscI AseI AsnI AspI
AviII BalI BbeI BbrPI BfrI
BglI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BsrBI BsrGI BssHII
BssSI Bst1107I BstZ17I ClaI CpoI
DraIII DrdI EaeI EagI EclXI
Eco52I FseI FspI HindIII HpaI
KasI KspI MfeI MluNI MscI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PflFI
PmaCI PmeI PmlI PshBI PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SnaBI
SphI Sse8387I SspBI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 2233 GACGTC GACGT^C
Acc65I 4014 GGTACC G^GTACC
AccIII 1904 TCCGGA T^CCGGA
AgeI 3290 ACCGGT A^CCGGT
Alw44I 4210 GTGCAC G^TGCAC
Aor51HI 901 AGCGCT AGC^GCT
ApaI 4484 GGGCCC GGGCC^C
ApaLI 4210 GTGCAC G^TGCAC
Asp718I 4014 GGTACC G^GTACC
BbvCI 2406 GCTGAGG CCTCAGC(-5/-2)
BcgI 3203 CGAGTCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2536 GGATAC GTATCC(6/5)
BclI 3164 TGATCA T^GATCA
BglII 3624 AGATCT A^GATCT
BsaAI 2305 TACGTG [CT]AC^GT[GA]
BsaI 253 GAGACC GGTCTC(1/5)
BseAI 1904 TCCGGA T^CCGGA
BspEI 1904 TCCGGA T^CCGGA
BsrFI 3290 ACCGGT [GA]^CCGG[CT]
BstAPI 836 GCAGTATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3331 TTCGAA TT^CGAA
BstEII 992 GGTCACC G^GT[ACGT]ACC
BstPI 992 GGTCACC G^GT[ACGT]ACC
Cfr10I 3290 ACCGGT [GA]^CCGG[CT]
Eco47III 901 AGCGCT AGC^GCT
EcoO65I 992 GGTCACC G^GT[ACGT]ACC
EcoRI 3790 GAATTC G^AATTC
EcoRV 1890 GATATC GAT^ATC
FbaI 3164 TGATCA T^GATCA
HaeII 901 AGCGCT [GA]GCGC^[CT]
KpnI 4014 GGTACC GGTAC^C
MluI 394 ACGCGT A^CGCGT
MroI 1904 TCCGGA T^CCGGA
NspV 3331 TTCGAA TT^CGAA
PinAI 3290 ACCGGT A^CCGGT
PshAI 3260 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2112 GAGCTC GAGCT^C
SfuI 3331 TTCGAA TT^CGAA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 135 CGCG CG^CG
395 CGCG
AhdI 46 GACTGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3900 GACCTTAGGTC
AocI 1635 CCTAAGG CC^T[ACGT]AGG
3902 CCTTAGG
AspEI 46 GACTGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3900 GACCTTAGGTC
AvrII 476 CCTAGG C^CTAGG
1818 CCTAGG
BamHI 701 GGATCC G^GATCC
2816 GGATCC
BanI 2547 GGTGCC G^G[CT][GA]CC
4014 GGTACC
BlnI 476 CCTAGG C^CTAGG
1818 CCTAGG
BlpI 1272 GCTGAGC GC^T[ACGT]AGC
3408 GCTAAGC
Bpu1102I 1272 GCTGAGC GC^T[ACGT]AGC
3408 GCTAAGC
BsaWI 1904 TCCGGA [AT]^CCGG[AT]
3290 ACCGGT
BsgI 56 CTGCAC GTGCAG(16/14)
766 CTGCAC
BspLU11I 3068 ACATGT A^CATGT
3555 ACATGT
BspMI 178 GCAGGT ACCTGC(4/8)
1257 GCAGGT
BstUI 135 CGCG CG^CG
395 CGCG
Bsu36I 1635 CCTAAGG CC^T[ACGT]AGG
3902 CCTTAGG
CelII 1272 GCTGAGC GC^T[ACGT]AGC
3408 GCTAAGC
Eam1105I 46 GACTGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3900 GACCTTAGGTC
Eco81I 1635 CCTAAGG CC^T[ACGT]AGG
3902 CCTTAGG
EcoNI 103 CCTCGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4393 CCTACTACAGG
EcoT22I 3828 ATGCAT ATGCA^T
4786 ATGCAT
HincII 1847 GTTGAC GT[CT]^[GA]AC
3233 GTCAAC
HindII 1847 GTTGAC GT[CT]^[GA]AC
3233 GTCAAC
MvnI 135 CGCG CG^CG
395 CGCG
NcoI 4018 CCATGG C^CATGG
4253 CCATGG
NsiI 3828 ATGCAT ATGCA^T
4786 ATGCAT
PflMI 2527 CCAATTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3450 CCACCACCTGG
PpuMI 3898 GGGACCT [GA]G^G[AT]CC[CT]
4350 AGGTCCT
ScaI 627 AGTACT AGT^ACT
4371 AGTACT
SpeI 4374 ACTAGT A^CTAGT
4774 ACTAGT
StuI 473 AGGCCT AGG^CCT
668 AGGCCT
Van91I 2527 CCAATTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3450 CCACCACCTGG
XcmI 3450 CCACCACCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4159 CCATGTGGGACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AclI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI Asp700I AspHI AvaI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaBI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PaeR7I PleI Psp1406I
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoI XhoII XmaCI
XmaI XmnI