##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AclI AflII AgeI AscI AseI
AsnI Asp700I AviII AvrII BbrPI
BfrI BlnI BlpI Bpu1102I BsiEI
BsiWI BspDI BspHI BspLU11I BssSI
CelII ClaI CpoI EagI EclXI
Eco52I EcoT22I FseI FspI HindIII
HpaI MfeI MluI MunI NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SfiI SnaBI
SpeI SspI SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 2248 GACGTC GACGT^C
Acc65I 3367 GGTACC G^GTACC
AccIII 2340 TCCGGA T^CCGGA
AflIII 1125 ACACGT A^C[GA][CT]GT
AhdI 1183 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3246 AGCGCT AGC^GCT
Asp718I 3367 GGTACC G^GTACC
AspEI 1183 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2717 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 1565 GTCTTC GAAGAC(2/6)
BcgI 1844 CGAGGGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4505 GTATCC GTATCC(6/5)
BclI 3153 TGATCA T^GATCA
BpuAI 1565 GTCTTC GAAGAC(2/6)
BseAI 2340 TCCGGA T^CCGGA
BsmBI 2444 GAGACG CGTCTC(1/5)
Bsp1407I 4517 TGTACA T^GTACA
BspEI 2340 TCCGGA T^CCGGA
BsrDI 4493 GCAATG GCAATG(2/0)
BsrGI 4517 TGTACA T^GTACA
Bst1107I 1159 GTATAC GTA^TAC
BstBI 720 TTCGAA TT^CGAA
BstZ17I 1159 GTATAC GTA^TAC
DraI 4565 TTTAAA TTT^AAA
Eam1105I 1183 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3246 AGCGCT AGC^GCT
EcoRV 521 GATATC GAT^ATC
FbaI 3153 TGATCA T^GATCA
HincII 1880 GTCAAC GT[CT]^[GA]AC
HindII 1880 GTCAAC GT[CT]^[GA]AC
KpnI 3367 GGTACC GGTAC^C
KspI 2734 CCGCGG CCGC^GG
MroI 2340 TCCGGA T^CCGGA
MseI 4566 TTAA T^TAA
NdeI 2634 CATATG CA^TATG
NspV 720 TTCGAA TT^CGAA
PflFI 2717 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 2734 CCGCGG CCGC^GG
SbfI 514 CCTGCAGG CCTGCA^GG
SexAI 3342 ACCTGGT A^CC[AT]GGT
SfuI 720 TTCGAA TT^CGAA
SgrAI 2981 CGCCGGCG C[GA]^CCGG[CT]G
SphI 2159 GCATGC GCATG^C
Sse8387I 514 CCTGCAGG CCTGCA^GG
SspBI 4517 TGTACA T^GTACA
Tru9I 4566 TTAA T^TAA
Tth111I 2717 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 370 GTGCAC G^TGCAC
1724 GTGCAC
ApaLI 370 GTGCAC G^TGCAC
1724 GTGCAC
BalI 2544 TGGCCA TGG^CCA
3231 TGGCCA
BbeI 1169 GGCGCC GGCGC^C
1610 GGCGCC
BglI 152 GCCCCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2646 GCCTTCTGGGC
BglII 735 AGATCT A^GATCT
1401 AGATCT
BsaAI 542 TACGTG [CT]AC^GT[GA]
2609 TACGTG
BsaBI 2311 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2428 GATGCGGATC
DraIII 1727 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
1870 CACCCAGTG
DrdI 1556 GACCTCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4309 GACTCTAGGGTC
EcoNI 439 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
677 CCTGTCACAGG
EcoRI 1596 GAATTC G^AATTC
3218 GAATTC
KasI 1169 GGCGCC G^GCGCC
1610 GGCGCC
MamI 2311 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2428 GATGCGGATC
MluNI 2544 TGGCCA TGG^CCA
3231 TGGCCA
MscI 2544 TGGCCA TGG^CCA
3231 TGGCCA
NaeI 1019 GCCGGC GCC^GGC
2982 GCCGGC
NarI 1169 GGCGCC GG^CGCC
1610 GGCGCC
NcoI 268 CCATGG C^CATGG
294 CCATGG
NgoMIV 1019 GCCGGC G^CCGGC
2982 GCCGGC
SfoI 1169 GGCGCC GGC^GCC
1610 GGCGCC
SmlI 82 CTTGAG C^T[CT][GA]AG
4216 CTTGAG
StuI 700 AGGCCT AGG^CCT
4115 AGGCCT
TaqI 721 TCGA T^CGA
3945 TCGA
TthHB8I 721 TCGA T^CGA
3945 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BamHI BanI BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmaI StyI
TfiI TseI Tsp45I Tsp509I TspRI
Van91I XcmI XhoII XmaCI XmaI