##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI ApoI
AscI AseI AsnI Asp718I AspEI
BamHI BbrPI BcgI BfrI BsaBI
BsiWI BspDI BspLU11I BsrBI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI Eam1105I EcoO65I EcoRI
EcoRV EcoT22I FseI KpnI KspI
MamI MfeI MluI MunI NdeI
NotI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI ScaI SfiI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 2414 TCCGGA T^CCGGA
Aor51HI 3774 AGCGCT AGC^GCT
AspI 2983 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3717 TGCGCA TGC^GCA
BciVI 706 GGATAC GTATCC(6/5)
BclI 1197 TGATCA T^GATCA
BglII 1031 AGATCT A^GATCT
BsaAI 2959 TACGTG [CT]AC^GT[GA]
BseAI 2414 TCCGGA T^CCGGA
BsmBI 2317 CGTCTC CGTCTC(1/5)
Bsp1407I 4041 TGTACA T^GTACA
BspEI 2414 TCCGGA T^CCGGA
BspHI 3498 TCATGA T^CATGA
BsrGI 4041 TGTACA T^GTACA
BssSI 2170 CTCGTG CACGAG(-5/-1)
DrdI 4285 GACCCCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3774 AGCGCT AGC^GCT
FbaI 1197 TGATCA T^GATCA
FspI 3717 TGCGCA TGC^GCA
HindIII 117 AAGCTT A^AGCTT
HpaI 1984 GTTAAC GTT^AAC
MaeII 2960 ACGT A^CGT
MroI 2414 TCCGGA T^CCGGA
NruI 2244 TCGCGA TCG^CGA
PflFI 2983 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 3498 TCATGA T^CATGA
SacI 3630 GAGCTC GAGCT^C
SmaI 3343 CCCGGG CCC^GGG
SspBI 4041 TGTACA T^GTACA
TaqI 4078 TCGA T^CGA
Tsp509I 4542 AATT ^AATT
Tth111I 2983 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 4078 TCGA T^CGA
XmaCI 3343 CCCGGG C^CCGGG
XmaI 3343 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AvaI 2469 CTCGGG C^[CT]CG[GA]G
3343 CCCGGG
AvrII 1675 CCTAGG C^CTAGG
3246 CCTAGG
BbeI 2238 GGCGCC GGCGC^C
3768 GGCGCC
BbsI 2596 GAAGAC GAAGAC(2/6)
4172 GAAGAC
BbvCI 273 CCTCAGC CCTCAGC(-5/-2)
3511 CCTCAGC
BlnI 1675 CCTAGG C^CTAGG
3246 CCTAGG
BlpI 1779 GCTAAGC GC^T[ACGT]AGC
3229 GCTCAGC
Bpu1102I 1779 GCTAAGC GC^T[ACGT]AGC
3229 GCTCAGC
BpuAI 2596 GAAGAC GAAGAC(2/6)
4172 GAAGAC
BsiEI 811 CGGCCG CG[GA][CT]^CG
2310 CGGCCG
BsmI 2521 GAATGC GAATGC(1/-1)
3818 GAATGC
BsoBI 2469 CTCGGG C^[CT]CG[GA]G
3343 CCCGGG
BstXI 165 CCACGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2094 CCATTCCCATGG
CelII 1779 GCTAAGC GC^T[ACGT]AGC
3229 GCTCAGC
DraIII 279 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
3800 CACTCCGTG
EagI 811 CGGCCG C^GGCCG
2310 CGGCCG
EclXI 811 CGGCCG C^GGCCG
2310 CGGCCG
Eco52I 811 CGGCCG C^GGCCG
2310 CGGCCG
HincII 1509 GTCAAC GT[CT]^[GA]AC
1984 GTTAAC
HindII 1509 GTCAAC GT[CT]^[GA]AC
1984 GTTAAC
KasI 2238 GGCGCC G^GCGCC
3768 GGCGCC
NaeI 1100 GCCGGC GCC^GGC
2312 GCCGGC
NarI 2238 GGCGCC GG^CGCC
3768 GGCGCC
NgoMIV 1100 GCCGGC G^CCGGC
2312 GCCGGC
PflMI 767 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2979 CCAGGACCTGG
SbfI 137 CCTGCAGG CCTGCA^GG
3099 CCTGCAGG
SexAI 1170 ACCTGGT A^CC[AT]GGT
2984 ACCTGGT
SfoI 2238 GGCGCC GGC^GCC
3768 GGCGCC
SphI 2384 GCATGC GCATG^C
3921 GCATGC
Sse8387I 137 CCTGCAGG CCTGCA^GG
3099 CCTGCAGG
TfiI 1544 GAATC G^A[AT]TC
2187 GAATC
Van91I 767 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2979 CCAGGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI Asp700I AspHI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrFI BsrI BssHII
BssKI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NheI NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI Tru9I TseI Tsp45I
TspRI XcmI XhoII XmnI