##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
ApaLI AscI AspI AvaI AviII
BamHI BbrPI BbvCI BcgI BglI
BsaAI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BsrBI BsrDI
BssHII BssSI BstEII BstPI ClaI
CpoI DsaI EagI EclXI Eco52I
EcoO65I EcoRV FseI FspI HpaI
KspI MluI MroI NaeI NcoI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI PvuII
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 2451 GGTACC G^GTACC
AccI 2659 GTATAC GT^[AC][GT]AC
AccII 762 CGCG CG^CG
AflII 3541 CTTAAG C^TTAAG
AhdI 1522 GACTTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1849 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 707 AGCGCT AGC^GCT
ApaI 4432 GGGCCC GGGCC^C
AseI 3468 ATTAAT AT^TAAT
AsnI 3468 ATTAAT AT^TAAT
Asp718I 2451 GGTACC G^GTACC
AspEI 1522 GACTTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 183 CCTAGG C^CTAGG
BbeI 1629 GGCGCC GGCGC^C
BciVI 3635 GTATCC GTATCC(6/5)
BclI 3357 TGATCA T^GATCA
BcnI 849 CCGGG CC^[GC]GG
BfrI 3541 CTTAAG C^TTAAG
BglII 410 AGATCT A^GATCT
BlnI 183 CCTAGG C^CTAGG
BsaI 3851 GAGACC GGTCTC(1/5)
BsaWI 4895 TCCGGT [AT]^CCGG[AT]
Bsp1407I 3572 TGTACA T^GTACA
BspLU11I 2570 ACATGT A^CATGT
BsrFI 2236 ACCGGC [GA]^CCGG[CT]
BsrGI 3572 TGTACA T^GTACA
Bst1107I 2659 GTATAC GTA^TAC
BstBI 3629 TTCGAA TT^CGAA
BstUI 762 CGCG CG^CG
BstZ17I 2659 GTATAC GTA^TAC
Cfr10I 2236 ACCGGC [GA]^CCGG[CT]
DraIII 3219 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2873 GACACTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1522 GACTTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 707 AGCGCT AGC^GCT
EcoNI 2747 CCTTCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1272 ATGCAT ATGCA^T
FbaI 3357 TGATCA T^GATCA
HgaI 1576 GACGC GACGC(5/10)
HincII 652 GTTGAC GT[CT]^[GA]AC
HindII 652 GTTGAC GT[CT]^[GA]AC
HindIII 1170 AAGCTT A^AGCTT
KasI 1629 GGCGCC G^GCGCC
KpnI 2451 GGTACC GGTAC^C
MfeI 1679 CAATTG C^AATTG
MunI 1679 CAATTG C^AATTG
MvnI 762 CGCG CG^CG
NarI 1629 GGCGCC GG^CGCC
NciI 849 CCGGG CC^[GC]GG
NsiI 1272 ATGCAT ATGCA^T
NspV 3629 TTCGAA TT^CGAA
PflMI 1848 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1893 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 3468 ATTAAT AT^TAAT
SfoI 1629 GGCGCC GGC^GCC
SfuI 3629 TTCGAA TT^CGAA
SspBI 3572 TGTACA T^GTACA
Van91I 1848 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 1576 GACGCC G[GA]^CG[CT]C
1629 GGCGCC
AflIII 1198 ACACGT A^C[GA][CT]GT
2570 ACATGT
BalI 791 TGGCCA TGG^CCA
1151 TGGCCA
BbsI 907 GTCTTC GAAGAC(2/6)
2147 GTCTTC
BlpI 804 GCTCAGC GC^T[ACGT]AGC
2103 GCTAAGC
Bpu1102I 804 GCTCAGC GC^T[ACGT]AGC
2103 GCTAAGC
BpuAI 907 GTCTTC GAAGAC(2/6)
2147 GTCTTC
BsaHI 1576 GACGCC G[GA]^CG[CT]C
1629 GGCGCC
BsmI 3782 GAATGC GAATGC(1/-1)
4780 GCATTC
BstAPI 331 GCAAACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
424 GCAACTTATGC
BstXI 1062 CCATTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3886 CCATGCCAGTGG
BstYI 410 AGATCT [GA]^GATC[CT]
2359 AGATCC
CelII 804 GCTCAGC GC^T[ACGT]AGC
2103 GCTAAGC
CfoI 708 GCGC GCG^C
1630 GCGC
EaeI 791 TGGCCA [CT]^GGCC[GA]
1151 TGGCCA
FauI 4442 CCCGC CCCGC(4/6)
1435 GCGGG
HaeII 707 AGCGCT [GA]GCGC^[CT]
1629 GGCGCC
HhaI 708 GCGC GCG^C
1630 GCGC
Hin1I 1576 GACGCC G[GA]^CG[CT]C
1629 GGCGCC
HinP1I 708 GCGC G^CGC
1630 GCGC
MflI 410 AGATCT [GA]^GATC[CT]
2359 AGATCC
MluNI 791 TGGCCA TGG^CCA
1151 TGGCCA
MscI 791 TGGCCA TGG^CCA
1151 TGGCCA
MspA1I 1478 CAGCGG C[AC]G^C[GT]G
4443 CCGCTG
NdeI 1187 CATATG CA^TATG
2681 CATATG
PstI 519 CTGCAG CTGCA^G
3376 CTGCAG
SacI 2600 GAGCTC GAGCT^C
4094 GAGCTC
XhoII 410 AGATCT [GA]^GATC[CT]
2359 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI Asp700I AspHI AvaII
BanI BanII BbvI BfaI BmyI
BpmI BsaBI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspMI BsrI BssKI
BstF5I BstNI Bsu36I Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
Eco81I EcoO109I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XmnI