##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccIII AclI AhdI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BcgI BlnI
BlpI Bpu1102I BsaBI BseAI BsgI
BsiWI BspDI BspEI BsrBI BssHII
BstBI BstEII BstPI Bsu36I CelII
ClaI CpoI Eam1105I Eco47III Eco81I
EcoO65I EcoRI FseI FspI KasI
KpnI MamI MluI MroI NaeI
NarI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SnaBI SphI Sse8387I
StuI TaqI Tth111I TthHB8I XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccI 2459 GTATAC GT^[AC][GT]AC
AcyI 2435 GACGCC G[GA]^CG[CT]C
AflII 1921 CTTAAG C^TTAAG
AgeI 2978 ACCGGT A^CCGGT
ApaI 2125 GGGCCC GGGCC^C
AseI 2160 ATTAAT AT^TAAT
AsnI 2160 ATTAAT AT^TAAT
AspHI 2844 GTGCTC G[AT]GC[AT]^C
AvaI 65 CCCGGG C^[CT]CG[GA]G
BalI 1064 TGGCCA TGG^CCA
BanI 3034 GGTGCC G^G[CT][GA]CC
BanII 2125 GGGCCC G[GA]GC[CT]^C
BbvCI 137 CCTCAGC CCTCAGC(-5/-2)
BclI 2816 TGATCA T^GATCA
BfrI 1921 CTTAAG C^TTAAG
BsaAI 929 TACGTG [CT]AC^GT[GA]
BsaHI 2435 GACGCC G[GA]^CG[CT]C
BsaWI 2978 ACCGGT [AT]^CCGG[AT]
BsiEI 143 CGGCCG CG[GA][CT]^CG
BsiHKAI 2844 GTGCTC G[AT]GC[AT]^C
BsmBI 79 CGTCTC CGTCTC(1/5)
BsmI 1858 GAATGC GAATGC(1/-1)
BsoBI 65 CCCGGG C^[CT]CG[GA]G
BspHI 3609 TCATGA T^CATGA
Bst1107I 2459 GTATAC GTA^TAC
BstAPI 3426 GCACAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2459 GTATAC GTA^TAC
CfoI 71 GCGC GCG^C
DraIII 2521 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4406 GACCTATCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 143 CGGCCG C^GGCCG
EclXI 143 CGGCCG C^GGCCG
Eco52I 143 CGGCCG C^GGCCG
EcoNI 2357 CCTTATAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2667 GATATC GAT^ATC
EcoT22I 1860 ATGCAT ATGCA^T
FbaI 2816 TGATCA T^GATCA
HaeII 70 GGCGCT [GA]GCGC^[CT]
HhaI 71 GCGC GCG^C
Hin1I 2435 GACGCC G[GA]^CG[CT]C
HinP1I 71 GCGC G^CGC
HindIII 2750 AAGCTT A^AGCTT
MluNI 1064 TGGCCA TGG^CCA
MscI 1064 TGGCCA TGG^CCA
NheI 858 GCTAGC G^CTAGC
NsiI 1860 ATGCAT ATGCA^T
PflMI 3714 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2978 ACCGGT A^CCGGT
PshAI 266 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2160 ATTAAT AT^TAAT
RcaI 3609 TCATGA T^CATGA
SapI 4075 GCTCTTC GCTCTTC(1/4)
SgrAI 2437 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 65 CCCGGG CCC^GGG
Van91I 3714 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3799 TCTAGA T^CTAGA
XcmI 1067 CCACCTATGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 65 CCCGGG C^CCGGG
XmaI 65 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 124 GATCC GGATC(4/5)
508 GATCC
AlwNI 731 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
2823 CAGTAACTG
Asp700I 652 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3510 GAAATATTTC
BciVI 1163 GGATAC GTATCC(6/5)
3709 GGATAC
BglI 136 GCCTCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1173 GCCAGCATGGC
BglII 952 AGATCT A^GATCT
4257 AGATCT
BpmI 82 CTCCAG CTGGAG(16/14)
323 CTGGAG
BsaI 2880 GAGACC GGTCTC(1/5)
1001 GGTCTC
BspMI 550 GCAGGT ACCTGC(4/8)
2148 GCAGGT
BssSI 1108 CACGAG CACGAG(-5/-1)
1825 CTCGTG
BstXI 425 CCAATTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1055 CCATTATGATGG
HpaI 1734 GTTAAC GTT^AAC
4444 GTTAAC
KspI 2 CCGCGG CCGC^GG
624 CCGCGG
MaeII 930 ACGT A^CGT
2967 ACGT
MfeI 2429 CAATTG C^AATTG
2849 CAATTG
MunI 2429 CAATTG C^AATTG
2849 CAATTG
NcoI 317 CCATGG C^CATGG
1158 CCATGG
NdeI 529 CATATG CA^TATG
716 CATATG
PpuMI 1569 AGGACCT [GA]G^G[AT]CC[CT]
4104 GGGACCC
PstI 30 CTGCAG CTGCA^G
268 CTGCAG
SacII 2 CCGCGG CCGC^GG
624 CCGCGG
SmlI 521 CTCAAG C^T[CT][GA]AG
1921 CTTAAG
SpeI 1777 ACTAGT A^CTAGT
1884 ACTAGT
SwaI 1725 ATTTAAAT ATTT^AAAT
4199 ATTTAAAT
XmnI 652 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3510 GAAATATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI ApoI AvaII BbsI BbvI
BcnI BfaI BmyI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspLU11I BsrDI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII