##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AclI AhdI AscI AseI AsnI
AspEI AspI BcgI BclI BglII
BsiWI Bsp1407I BspDI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI Eam1105I EcoRV EcoT22I
FbaI MfeI MluI MunI NdeI
NheI NotI NsiI NspV PacI
PaeR7I PflFI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SbfI
ScaI SexAI SfiI SfuI SnaBI
SphI Sse8387I SspBI SspI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 164 GACGTC GACGT^C
Acc65I 1828 GGTACC G^GTACC
AccIII 120 TCCGGA T^CCGGA
AflII 612 CTTAAG C^TTAAG
AgeI 1684 ACCGGT A^CCGGT
AocI 4057 CCTGAGG CC^T[ACGT]AGG
Aor51HI 237 AGCGCT AGC^GCT
ApaI 2337 GGGCCC GGGCC^C
Asp700I 1814 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1828 GGTACC G^GTACC
AvrII 1977 CCTAGG C^CTAGG
BbeI 1295 GGCGCC GGCGC^C
BbsI 3063 GTCTTC GAAGAC(2/6)
BfrI 612 CTTAAG C^TTAAG
BlnI 1977 CCTAGG C^CTAGG
BpuAI 3063 GTCTTC GAAGAC(2/6)
BsaBI 840 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 120 TCCGGA T^CCGGA
BspEI 120 TCCGGA T^CCGGA
BspHI 836 TCATGA T^CATGA
BsrBI 19 GAGCGG CCGCTC(-3/-3)
Bsu36I 4057 CCTGAGG CC^T[ACGT]AGG
DraI 3662 TTTAAA TTT^AAA
DraIII 2080 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1727 GACACAGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 355 CGGCCG C^GGCCG
EclXI 355 CGGCCG C^GGCCG
Eco47III 237 AGCGCT AGC^GCT
Eco52I 355 CGGCCG C^GGCCG
Eco81I 4057 CCTGAGG CC^T[ACGT]AGG
FseI 2172 GGCCGGCC GGCCGG^CC
HpaI 1688 GTTAAC GTT^AAC
KasI 1295 GGCGCC G^GCGCC
KpnI 1828 GGTACC GGTAC^C
MamI 840 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 120 TCCGGA T^CCGGA
NarI 1295 GGCGCC GG^CGCC
NruI 4083 TCGCGA TCG^CGA
PflMI 1593 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1684 ACCGGT A^CCGGT
PstI 3136 CTGCAG CTGCA^G
RcaI 836 TCATGA T^CATGA
SapI 3384 GAAGAGC GCTCTTC(1/4)
SfoI 1295 GGCGCC GGC^GCC
SgrAI 79 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 4071 ACTAGT A^CTAGT
Van91I 1593 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1814 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 167 GTCTAC GT^[AC][GT]AC
1889 GTCTAC
AflIII 729 ACGTGT A^C[GA][CT]GT
1157 ACGTGT
AviII 1570 TGCGCA TGC^GCA
2072 TGCGCA
BalI 1301 TGGCCA TGG^CCA
3578 TGGCCA
BamHI 656 GGATCC G^GATCC
2414 GGATCC
BbrPI 269 CACGTG CAC^GTG
1156 CACGTG
BciVI 1320 GGATAC GTATCC(6/5)
1930 GGATAC
BsaAI 269 CACGTG [CT]AC^GT[GA]
1156 CACGTG
BsaWI 120 TCCGGA [AT]^CCGG[AT]
1684 ACCGGT
BsiEI 99 CGACCG CG[GA][CT]^CG
355 CGGCCG
BsmI 3481 GAATGC GAATGC(1/-1)
3980 GAATGC
BstEII 91 GGTGACC G^GT[ACGT]ACC
487 GGTTACC
BstPI 91 GGTGACC G^GT[ACGT]ACC
487 GGTTACC
EcoO65I 91 GGTGACC G^GT[ACGT]ACC
487 GGTTACC
EcoRI 2890 GAATTC G^AATTC
3896 GAATTC
FspI 1570 TGCGCA TGC^GCA
2072 TGCGCA
HindIII 545 AAGCTT A^AGCTT
1032 AAGCTT
KspI 1704 CCGCGG CCGC^GG
2110 CCGCGG
MluNI 1301 TGGCCA TGG^CCA
3578 TGGCCA
MscI 1301 TGGCCA TGG^CCA
3578 TGGCCA
NcoI 1807 CCATGG C^CATGG
4064 CCATGG
PmaCI 269 CACGTG CAC^GTG
1156 CACGTG
PmlI 269 CACGTG CAC^GTG
1156 CACGTG
PpuMI 1218 GGGTCCT [GA]G^G[AT]CC[CT]
2256 GGGACCC
SacII 1704 CCGCGG CCGC^GG
2110 CCGCGG
SmaI 2217 CCCGGG CCC^GGG
3148 CCCGGG
TaqI 517 TCGA T^CGA
3093 TCGA
TthHB8I 517 TCGA T^CGA
3093 TCGA
XbaI 1360 TCTAGA T^CTAGA
4077 TCTAGA
XcmI 1926 CCAAGGATACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2272 CCAGGCAAGGCGTGG
XmaCI 2217 CCCGGG C^CCGGG
3148 CCCGGG
XmaI 2217 CCCGGG C^CCGGG
3148 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PleI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII