##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AhdI Alw44I Aor51HI ApaLI AscI
AspEI AviII BbrPI BfrI BglII
BseAI BsiWI BsmBI BspEI BspHI
BspLU11I Bst1107I BstBI BstZ17I CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoRI FseI FspI HpaI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NspV PacI
PaeR7I PmaCI PmeI PmlI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcyI 1004 GGCGCC G[GA]^CG[CT]C
AgeI 2017 ACCGGT A^CCGGT
AocI 1497 CCTGAGG CC^T[ACGT]AGG
AseI 3252 ATTAAT AT^TAAT
AsnI 3252 ATTAAT AT^TAAT
AspI 1353 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3567 GGATCC G^GATCC
BbeI 1004 GGCGCC GGCGC^C
BcgI 3535 CGAGGAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4214 GTATCC GTATCC(6/5)
BglI 708 GCCAATGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 158 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 158 GCTGAGC GC^T[ACGT]AGC
BsaAI 3106 TACGTG [CT]AC^GT[GA]
BsaHI 1004 GGCGCC G[GA]^CG[CT]C
BsaI 905 GGTCTC GGTCTC(1/5)
BsiEI 400 CGACCG CG[GA][CT]^CG
BsmI 2827 GCATTC GAATGC(1/-1)
BspDI 3219 ATCGAT AT^CGAT
BspMI 1484 GCAGGT ACCTGC(4/8)
BssHII 3676 GCGCGC G^CGCGC
BstAPI 2198 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1497 CCTGAGG CC^T[ACGT]AGG
CelII 158 GCTGAGC GC^T[ACGT]AGC
ClaI 3219 ATCGAT AT^CGAT
Eco81I 1497 CCTGAGG CC^T[ACGT]AGG
EcoNI 372 CCTCCCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1331 GATATC GAT^ATC
EcoT22I 1770 ATGCAT ATGCA^T
Hin1I 1004 GGCGCC G[GA]^CG[CT]C
KasI 1004 GGCGCC G^GCGCC
NarI 1004 GGCGCC GG^CGCC
NsiI 1770 ATGCAT ATGCA^T
PflFI 1353 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2017 ACCGGT A^CCGGT
PshAI 876 GACGAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3252 ATTAAT AT^TAAT
SacI 408 GAGCTC GAGCT^C
SfoI 1004 GGCGCC GGC^GCC
SmaI 2762 CCCGGG CCC^GGG
SphI 3848 GCATGC GCATG^C
Tth111I 1353 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2762 CCCGGG C^CCGGG
XmaI 2762 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 2959 GGTACC G^GTACC
4141 GGTACC
AcsI 1899 GAATTT [GA]^AATT[CT]
3992 GAATTT
AflIII 1828 ACGTGT A^C[GA][CT]GT
2047 ACACGT
ApaI 123 GGGCCC GGGCC^C
3880 GGGCCC
ApoI 1899 GAATTT [GA]^AATT[CT]
3992 GAATTT
Asp700I 2882 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3294 GAATACCTTC
Asp718I 2959 GGTACC G^GTACC
4141 GGTACC
AvaI 2517 CCCGAG C^[CT]CG[GA]G
2762 CCCGGG
BalI 2855 TGGCCA TGG^CCA
3368 TGGCCA
BclI 2572 TGATCA T^GATCA
3280 TGATCA
BsaWI 2017 ACCGGT [AT]^CCGG[AT]
2890 TCCGGT
BseRI 11 GAGGAG GAGGAG(10/8)
3760 GAGGAG
BsoBI 2517 CCCGAG C^[CT]CG[GA]G
2762 CCCGGG
BsrBI 161 GAGCGG CCGCTC(-3/-3)
3171 GAGCGG
BssSI 3533 CACGAG CACGAG(-5/-1)
3947 CTCGTG
DraI 612 TTTAAA TTT^AAA
1455 TTTAAA
EarI 3087 GAAGAG CTCTTC(1/4)
4102 GAAGAG
FbaI 2572 TGATCA T^GATCA
3280 TGATCA
HaeII 1004 GGCGCC [GA]GCGC^[CT]
3176 GGCGCT
HgaI 3590 GACGC GACGC(5/10)
3852 GCGTC
HindIII 2726 AAGCTT A^AGCTT
3318 AAGCTT
KpnI 2959 GGTACC GGTAC^C
4141 GGTACC
Ksp632I 3087 GAAGAG CTCTTC(1/4)
4102 GAAGAG
KspI 127 CCGCGG CCGC^GG
142 CCGCGG
MluNI 2855 TGGCCA TGG^CCA
3368 TGGCCA
MscI 2855 TGGCCA TGG^CCA
3368 TGGCCA
NcoI 3028 CCATGG C^CATGG
4132 CCATGG
NspI 1861 ACATGC [GA]CATG^[CT]
3848 GCATGC
PflMI 852 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1064 CCACGTCATGG
PvuII 1287 CAGCTG CAG^CTG
2220 CAGCTG
SacII 127 CCGCGG CCGC^GG
142 CCGCGG
StuI 2489 AGGCCT AGG^CCT
2704 AGGCCT
Van91I 852 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1064 CCACGTCATGG
XcmI 2087 CCACCATGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3028 CCATGGAAGCGGTGG
XmnI 2882 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3294 GAATACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII AvrII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BlnI BmyI BpmI BpuAI
BsaBI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrDI BsrFI BsrGI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII