##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AclI AflII AlwNI
AocI AscI AseI AsnI Asp700I
Asp718I AspI BclI BfrI BglII
BsaBI BsiWI Bsp1407I BspDI BsrGI
Bst1107I BstBI BstZ17I Bsu36I ClaI
DrdI EagI EclXI Eco52I Eco81I
EcoRI FbaI FseI KpnI MamI
MfeI MluI MunI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PmeI PshAI PshBI
Psp1406I PvuI SapI SbfI ScaI
SexAI SfuI SnaBI SphI Sse8387I
SspBI SspI SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccIII 38 TCCGGA T^CCGGA
AflIII 3771 ACATGT A^C[GA][CT]GT
AgeI 3182 ACCGGT A^CCGGT
AhdI 3885 GACTCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 982 GTGCAC G^TGCAC
Aor51HI 2607 AGCGCT AGC^GCT
ApaLI 982 GTGCAC G^TGCAC
AspEI 3885 GACTCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1225 TGCGCA TGC^GCA
AvrII 1273 CCTAGG C^CTAGG
BalI 669 TGGCCA TGG^CCA
BamHI 3402 GGATCC G^GATCC
BbeI 2924 GGCGCC GGCGC^C
BbrPI 2303 CACGTG CAC^GTG
BbvCI 2384 GCTGAGG CCTCAGC(-5/-2)
BlnI 1273 CCTAGG C^CTAGG
BlpI 2795 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2795 GCTAAGC GC^T[ACGT]AGC
BsaAI 2303 CACGTG [CT]AC^GT[GA]
BseAI 38 TCCGGA T^CCGGA
BsiEI 159 CGGTCG CG[GA][CT]^CG
BsmBI 1574 CGTCTC CGTCTC(1/5)
BspEI 38 TCCGGA T^CCGGA
BspHI 3037 TCATGA T^CATGA
BspLU11I 3771 ACATGT A^CATGT
BspMI 2071 ACCTGC ACCTGC(4/8)
BsrDI 66 CATTGC GCAATG(2/0)
BssSI 2062 CTCGTG CACGAG(-5/-1)
BstAPI 1231 GCACGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3839 GGTCACC G^GT[ACGT]ACC
BstPI 3839 GGTCACC G^GT[ACGT]ACC
CelII 2795 GCTAAGC GC^T[ACGT]AGC
CpoI 35 CGGTCCG CG^G[AT]CCG
Eam1105I 3885 GACTCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2607 AGCGCT AGC^GCT
EcoO65I 3839 GGTCACC G^GT[ACGT]ACC
EcoRV 1211 GATATC GAT^ATC
EcoT22I 3757 ATGCAT ATGCA^T
FspI 1225 TGCGCA TGC^GCA
HindIII 2708 AAGCTT A^AGCTT
HpaI 3767 GTTAAC GTT^AAC
KasI 2924 GGCGCC G^GCGCC
KspI 3700 CCGCGG CCGC^GG
MluNI 669 TGGCCA TGG^CCA
MroI 38 TCCGGA T^CCGGA
MscI 669 TGGCCA TGG^CCA
NarI 2924 GGCGCC GG^CGCC
NsiI 3757 ATGCAT ATGCA^T
NspI 3771 ACATGT [GA]CATG^[CT]
PinAI 3182 ACCGGT A^CCGGT
PmaCI 2303 CACGTG CAC^GTG
PmlI 2303 CACGTG CAC^GTG
PstI 2183 CTGCAG CTGCA^G
RcaI 3037 TCATGA T^CATGA
RsrII 35 CGGTCCG CG^G[AT]CCG
SacII 3700 CCGCGG CCGC^GG
SalI 722 GTCGAC G^TCGAC
SfiI 3782 GGCCCTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2924 GGCGCC GGC^GCC
SgrAI 3181 CACCGGTG C[GA]^CCGG[CT]G
SpeI 1795 ACTAGT A^CTAGT
XbaI 1189 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 722 GTCGAC GT^[AC][GT]AC
4204 GTAGAC
ApaI 2210 GGGCCC GGGCC^C
2276 GGGCCC
BanI 2924 GGCGCC G^G[CT][GA]CC
3185 GGTGCC
BciVI 4028 GGATAC GTATCC(6/5)
3961 GTATCC
BsaWI 38 TCCGGA [AT]^CCGG[AT]
3182 ACCGGT
BsrBI 24 CCGCTC CCGCTC(-3/-3)
389 GAGCGG
BsrFI 2951 GCCGGT [GA]^CCGG[CT]
3182 ACCGGT
Cfr10I 2951 GCCGGT [GA]^CCGG[CT]
3182 ACCGGT
DraI 4010 TTTAAA TTT^AAA
4124 TTTAAA
EcoNI 580 CCTACTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
646 CCTGGACAAGG
NcoI 2932 CCATGG C^CATGG
3344 CCATGG
PaeR7I 1025 CTCGAG C^TCGAG
2362 CTCGAG
PflMI 310 CCATGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
367 CCAGAACATGG
PpuMI 975 GGGACCT [GA]G^G[AT]CC[CT]
1003 GGGTCCC
SmaI 3387 CCCGGG CCC^GGG
3590 CCCGGG
StuI 2268 AGGCCT AGG^CCT
2679 AGGCCT
TfiI 1441 GATTC G^A[AT]TC
1517 GAATC
Van91I 310 CCATGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
367 CCAGAACATGG
XhoI 1025 CTCGAG C^TCGAG
2362 CTCGAG
XmaCI 3387 CCCGGG C^CCGGG
3590 CCCGGG
XmaI 3387 CCCGGG C^CCGGG
3590 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaI
AvaII BanII BbsI BbvI BcgI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrI
BssHII BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII