################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII Acc65I AclI AflII AlwNI AocI AscI AseI AsnI Asp700I Asp718I AspI BclI BfrI BglII BsaBI BsiWI Bsp1407I BspDI BsrGI Bst1107I BstBI BstZ17I Bsu36I ClaI DrdI EagI EclXI Eco52I Eco81I EcoRI FbaI FseI KpnI MamI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PmeI PshAI PshBI Psp1406I PvuI SapI SbfI ScaI SexAI SfuI SnaBI SphI Sse8387I SspBI SspI SwaI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccIII 38 TCCGGA T^CCGGA AflIII 3771 ACATGT A^C[GA][CT]GT AgeI 3182 ACCGGT A^CCGGT AhdI 3885 GACTCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 982 GTGCAC G^TGCAC Aor51HI 2607 AGCGCT AGC^GCT ApaLI 982 GTGCAC G^TGCAC AspEI 3885 GACTCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1225 TGCGCA TGC^GCA AvrII 1273 CCTAGG C^CTAGG BalI 669 TGGCCA TGG^CCA BamHI 3402 GGATCC G^GATCC BbeI 2924 GGCGCC GGCGC^C BbrPI 2303 CACGTG CAC^GTG BbvCI 2384 GCTGAGG CCTCAGC(-5/-2) BlnI 1273 CCTAGG C^CTAGG BlpI 2795 GCTAAGC GC^T[ACGT]AGC Bpu1102I 2795 GCTAAGC GC^T[ACGT]AGC BsaAI 2303 CACGTG [CT]AC^GT[GA] BseAI 38 TCCGGA T^CCGGA BsiEI 159 CGGTCG CG[GA][CT]^CG BsmBI 1574 CGTCTC CGTCTC(1/5) BspEI 38 TCCGGA T^CCGGA BspHI 3037 TCATGA T^CATGA BspLU11I 3771 ACATGT A^CATGT BspMI 2071 ACCTGC ACCTGC(4/8) BsrDI 66 CATTGC GCAATG(2/0) BssSI 2062 CTCGTG CACGAG(-5/-1) BstAPI 1231 GCACGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3839 GGTCACC G^GT[ACGT]ACC BstPI 3839 GGTCACC G^GT[ACGT]ACC CelII 2795 GCTAAGC GC^T[ACGT]AGC CpoI 35 CGGTCCG CG^G[AT]CCG Eam1105I 3885 GACTCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2607 AGCGCT AGC^GCT EcoO65I 3839 GGTCACC G^GT[ACGT]ACC EcoRV 1211 GATATC GAT^ATC EcoT22I 3757 ATGCAT ATGCA^T FspI 1225 TGCGCA TGC^GCA HindIII 2708 AAGCTT A^AGCTT HpaI 3767 GTTAAC GTT^AAC KasI 2924 GGCGCC G^GCGCC KspI 3700 CCGCGG CCGC^GG MluNI 669 TGGCCA TGG^CCA MroI 38 TCCGGA T^CCGGA MscI 669 TGGCCA TGG^CCA NarI 2924 GGCGCC GG^CGCC NsiI 3757 ATGCAT ATGCA^T NspI 3771 ACATGT [GA]CATG^[CT] PinAI 3182 ACCGGT A^CCGGT PmaCI 2303 CACGTG CAC^GTG PmlI 2303 CACGTG CAC^GTG PstI 2183 CTGCAG CTGCA^G RcaI 3037 TCATGA T^CATGA RsrII 35 CGGTCCG CG^G[AT]CCG SacII 3700 CCGCGG CCGC^GG SalI 722 GTCGAC G^TCGAC SfiI 3782 GGCCCTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 2924 GGCGCC GGC^GCC SgrAI 3181 CACCGGTG C[GA]^CCGG[CT]G SpeI 1795 ACTAGT A^CTAGT XbaI 1189 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 722 GTCGAC GT^[AC][GT]AC 4204 GTAGAC ApaI 2210 GGGCCC GGGCC^C 2276 GGGCCC BanI 2924 GGCGCC G^G[CT][GA]CC 3185 GGTGCC BciVI 4028 GGATAC GTATCC(6/5) 3961 GTATCC BsaWI 38 TCCGGA [AT]^CCGG[AT] 3182 ACCGGT BsrBI 24 CCGCTC CCGCTC(-3/-3) 389 GAGCGG BsrFI 2951 GCCGGT [GA]^CCGG[CT] 3182 ACCGGT Cfr10I 2951 GCCGGT [GA]^CCGG[CT] 3182 ACCGGT DraI 4010 TTTAAA TTT^AAA 4124 TTTAAA EcoNI 580 CCTACTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 646 CCTGGACAAGG NcoI 2932 CCATGG C^CATGG 3344 CCATGG PaeR7I 1025 CTCGAG C^TCGAG 2362 CTCGAG PflMI 310 CCATGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 367 CCAGAACATGG PpuMI 975 GGGACCT [GA]G^G[AT]CC[CT] 1003 GGGTCCC SmaI 3387 CCCGGG CCC^GGG 3590 CCCGGG StuI 2268 AGGCCT AGG^CCT 2679 AGGCCT TfiI 1441 GATTC G^A[AT]TC 1517 GAATC Van91I 310 CCATGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 367 CCAGAACATGG XhoI 1025 CTCGAG C^TCGAG 2362 CTCGAG XmaCI 3387 CCCGGG C^CCGGG 3590 CCCGGG XmaI 3387 CCCGGG C^CCGGG 3590 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI ApoI AspHI AvaI AvaII BanII BbsI BbvI BcgI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrI BssHII BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII