##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI AspEI AspI AviII AvrII
BbrPI BciVI BclI BfrI BlnI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI MluI MroI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI SfuI SgrAI SnaBI SphI
Sse8387I SspBI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 3409 GGTACC G^GTACC
Asp700I 3874 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3409 GGTACC G^GTACC
BamHI 3900 GGATCC G^GATCC
BcgI 3170 CGAGTTTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3310 AGATCT A^GATCT
BsaAI 3588 CACGTA [CT]AC^GT[GA]
BsaI 3339 GAGACC GGTCTC(1/5)
BsaWI 3098 ACCGGA [AT]^CCGG[AT]
BsmI 2399 GAATGC GAATGC(1/-1)
BsrBI 70 GAGCGG CCGCTC(-3/-3)
BsrDI 2924 CATTGC GCAATG(2/0)
BsrFI 1558 ACCGGC [GA]^CCGG[CT]
Cfr10I 1558 ACCGGC [GA]^CCGG[CT]
DraI 3560 TTTAAA TTT^AAA
DraIII 1192 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
HgaI 234 GACGC GACGC(5/10)
KpnI 3409 GGTACC GGTAC^C
MfeI 562 CAATTG C^AATTG
MunI 562 CAATTG C^AATTG
SacI 692 GAGCTC GAGCT^C
SapI 2357 GAAGAGC GCTCTTC(1/4)
ScaI 2734 AGTACT AGT^ACT
SexAI 3405 ACCTGGT A^CC[AT]GGT
SfiI 2030 GGCCCATTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 2423 ACTAGT A^CTAGT
XcmI 3816 CCAGTGTAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3874 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 3213 GAATTT [GA]^AATT[CT]
3874 GAATTT
AflIII 2138 ACATGT A^C[GA][CT]GT
3970 ACATGT
ApaI 57 GGGCCC GGGCC^C
3296 GGGCCC
ApoI 3213 GAATTT [GA]^AATT[CT]
3874 GAATTT
AvaI 240 CCCGGG C^[CT]CG[GA]G
1996 CCCGGG
BalI 646 TGGCCA TGG^CCA
1364 TGGCCA
BbeI 106 GGCGCC GGCGC^C
127 GGCGCC
BsaBI 933 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1526 GATTGCCATC
BsoBI 240 CCCGGG C^[CT]CG[GA]G
1996 CCCGGG
BspLU11I 2138 ACATGT A^CATGT
3970 ACATGT
BstXI 325 CCACTATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2060 CCAGGCTCCTGG
EagI 52 CGGCCG C^GGCCG
658 CGGCCG
EclXI 52 CGGCCG C^GGCCG
658 CGGCCG
Eco52I 52 CGGCCG C^GGCCG
658 CGGCCG
HaeII 106 GGCGCC [GA]GCGC^[CT]
127 GGCGCC
KasI 106 GGCGCC G^GCGCC
127 GGCGCC
MaeII 3062 ACGT A^CGT
3589 ACGT
MamI 933 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1526 GATTGCCATC
MluNI 646 TGGCCA TGG^CCA
1364 TGGCCA
MscI 646 TGGCCA TGG^CCA
1364 TGGCCA
NarI 106 GGCGCC GG^CGCC
127 GGCGCC
NspI 2138 ACATGT [GA]CATG^[CT]
3970 ACATGT
SfoI 106 GGCGCC GGC^GCC
127 GGCGCC
SmaI 240 CCCGGG CCC^GGG
1996 CCCGGG
SspI 4148 AATATT AAT^ATT
4155 AATATT
TaqI 636 TCGA T^CGA
1928 TCGA
TthHB8I 636 TCGA T^CGA
1928 TCGA
XbaI 2478 TCTAGA T^CTAGA
3623 TCTAGA
XmaCI 240 CCCGGG C^CCGGG
1996 CCCGGG
XmaI 240 CCCGGG C^CCGGG
1996 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaLI
AspHI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII