##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI AspEI AspI AviII AvrII
BbeI BciVI BfrI BlnI BsaBI
BsaHI BsiWI BsmBI Bst1107I BstZ17I
Bsu36I CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV FseI FspI Hin1I
HincII HindII HpaI KasI MamI
MfeI MluI MunI NarI NheI
NotI NruI PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI SexAI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 3586 GGTACC G^GTACC
AccI 514 GTCTAC GT^[AC][GT]AC
AflIII 4013 ACATGT A^C[GA][CT]GT
Asp700I 2451 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3586 GGTACC G^GTACC
BamHI 3696 GGATCC G^GATCC
BcgI 14 GCACCCAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3405 TGATCA T^GATCA
BglII 669 AGATCT A^GATCT
BsiEI 276 CGACCG CG[GA][CT]^CG
Bsp1407I 196 TGTACA T^GTACA
BspDI 1385 ATCGAT AT^CGAT
BspHI 1011 TCATGA T^CATGA
BspLU11I 4013 ACATGT A^CATGT
BsrBI 38 GAGCGG CCGCTC(-3/-3)
BsrGI 196 TGTACA T^GTACA
BssHII 155 GCGCGC G^CGCGC
BstBI 1187 TTCGAA TT^CGAA
ClaI 1385 ATCGAT AT^CGAT
EcoT22I 3109 ATGCAT ATGCA^T
FbaI 3405 TGATCA T^GATCA
KpnI 3586 GGTACC GGTAC^C
KspI 2399 CCGCGG CCGC^GG
NaeI 856 GCCGGC GCC^GGC
NdeI 3889 CATATG CA^TATG
NgoMIV 856 GCCGGC G^CCGGC
NsiI 3109 ATGCAT ATGCA^T
NspV 1187 TTCGAA TT^CGAA
PaeR7I 3418 CTCGAG C^TCGAG
PvuII 2378 CAGCTG CAG^CTG
RcaI 1011 TCATGA T^CATGA
SacI 3152 GAGCTC GAGCT^C
SacII 2399 CCGCGG CCGC^GG
SapI 2481 GAAGAGC GCTCTTC(1/4)
ScaI 302 AGTACT AGT^ACT
SfuI 1187 TTCGAA TT^CGAA
SmaI 2657 CCCGGG CCC^GGG
SspBI 196 TGTACA T^GTACA
XbaI 3227 TCTAGA T^CTAGA
XhoI 3418 CTCGAG C^TCGAG
XmaCI 2657 CCCGGG C^CCGGG
XmaI 2657 CCCGGG C^CCGGG
XmnI 2451 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 1097 TCCGGA T^CCGGA
2533 TCCGGA
Alw44I 913 GTGCAC G^TGCAC
3090 GTGCAC
ApaLI 913 GTGCAC G^TGCAC
3090 GTGCAC
BalI 1196 TGGCCA TGG^CCA
3709 TGGCCA
BbrPI 1639 CACGTG CAC^GTG
2635 CACGTG
BlpI 2677 GCTCAGC GC^T[ACGT]AGC
3545 GCTGAGC
Bpu1102I 2677 GCTCAGC GC^T[ACGT]AGC
3545 GCTGAGC
BsaAI 1639 CACGTG [CT]AC^GT[GA]
2635 CACGTG
BseAI 1097 TCCGGA T^CCGGA
2533 TCCGGA
BspEI 1097 TCCGGA T^CCGGA
2533 TCCGGA
BspMI 3038 GCAGGT ACCTGC(4/8)
3244 GCAGGT
BsrDI 949 GCAATG GCAATG(2/0)
3894 GCAATG
BsrFI 856 GCCGGC [GA]^CCGG[CT]
3583 GCCGGT
BstEII 228 GGTGACC G^GT[ACGT]ACC
3563 GGTGACC
BstPI 228 GGTGACC G^GT[ACGT]ACC
3563 GGTGACC
BstXI 2138 CCATCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3517 CCAAGAGGCTGG
CelII 2677 GCTCAGC GC^T[ACGT]AGC
3545 GCTGAGC
Cfr10I 856 GCCGGC [GA]^CCGG[CT]
3583 GCCGGT
DrdI 1216 GACAACTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3449 GACATCACCGTC
EcoO65I 228 GGTGACC G^GT[ACGT]ACC
3563 GGTGACC
HaeII 1 AGCGCC [GA]GCGC^[CT]
984 GGCGCT
HgaI 908 GCGTC GACGC(5/10)
2557 GCGTC
HindIII 1565 AAGCTT A^AGCTT
3611 AAGCTT
MluNI 1196 TGGCCA TGG^CCA
3709 TGGCCA
MroI 1097 TCCGGA T^CCGGA
2533 TCCGGA
MscI 1196 TGGCCA TGG^CCA
3709 TGGCCA
NcoI 1020 CCATGG C^CATGG
1104 CCATGG
PflMI 3049 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3701 CCACTGATTGG
PmaCI 1639 CACGTG CAC^GTG
2635 CACGTG
PmlI 1639 CACGTG CAC^GTG
2635 CACGTG
PstI 1654 CTGCAG CTGCA^G
2801 CTGCAG
SmlI 306 CTTGAG C^T[CT][GA]AG
3418 CTCGAG
Van91I 3049 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3701 CCACTGATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII