################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII ApoI AscI Asp718I BciVI BfrI BsiWI BsmI BspLU11I BsrDI BssHII Bst1107I BstBI BstZ17I CpoI DraI DraIII EarI EcoRI EcoRV EcoT22I HindIII HpaI KpnI Ksp632I MfeI MluI MunI NcoI NdeI NheI NotI NsiI NspV PacI PaeR7I PmeI PshAI Psp1406I PvuI RsrII SalI SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 1037 TCCGGA T^CCGGA AgeI 884 ACCGGT A^CCGGT Alw44I 731 GTGCAC G^TGCAC ApaLI 731 GTGCAC G^TGCAC AseI 1532 ATTAAT AT^TAAT AsnI 1532 ATTAAT AT^TAAT Asp700I 1660 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 2900 TGCGCA TGC^GCA AvrII 1503 CCTAGG C^CTAGG BamHI 2723 GGATCC G^GATCC BbsI 997 GAAGAC GAAGAC(2/6) BclI 2874 TGATCA T^GATCA BglII 7 AGATCT A^GATCT BlnI 1503 CCTAGG C^CTAGG BlpI 1848 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1848 GCTGAGC GC^T[ACGT]AGC BpuAI 997 GAAGAC GAAGAC(2/6) BsaBI 1726 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1037 TCCGGA T^CCGGA Bsp1407I 3327 TGTACA T^GTACA BspDI 2837 ATCGAT AT^CGAT BspEI 1037 TCCGGA T^CCGGA BspHI 1703 TCATGA T^CATGA BsrGI 3327 TGTACA T^GTACA BssSI 3455 CACGAG CACGAG(-5/-1) BstAPI 2974 GCATGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 1848 GCTGAGC GC^T[ACGT]AGC ClaI 2837 ATCGAT AT^CGAT FbaI 2874 TGATCA T^GATCA FseI 2515 GGCCGGCC GGCCGG^CC FspI 2900 TGCGCA TGC^GCA HincII 3803 GTCAAC GT[CT]^[GA]AC HindII 3803 GTCAAC GT[CT]^[GA]AC KspI 662 CCGCGG CCGC^GG MamI 1726 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1037 TCCGGA T^CCGGA NruI 3773 TCGCGA TCG^CGA PinAI 884 ACCGGT A^CCGGT PshBI 1532 ATTAAT AT^TAAT RcaI 1703 TCATGA T^CATGA SacII 662 CCGCGG CCGC^GG ScaI 2820 AGTACT AGT^ACT SphI 2974 GCATGC GCATG^C SspBI 3327 TGTACA T^GTACA StuI 1274 AGGCCT AGG^CCT Tsp509I 3842 AATT ^AATT XcmI 1604 CCAGGGCAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1660 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AhdI 148 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3551 GACAGCAAGTC Aor51HI 555 AGCGCT AGC^GCT 3063 AGCGCT AspEI 148 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3551 GACAGCAAGTC BbrPI 1028 CACGTG CAC^GTG 3861 CACGTG BsaAI 1028 CACGTG [CT]AC^GT[GA] 3861 CACGTG BsgI 1887 CTGCAC GTGCAG(16/14) 3953 CTGCAC BsiEI 294 CGGCCG CG[GA][CT]^CG 3386 CGGCCG BsrBI 2705 CCGCTC CCGCTC(-3/-3) 1697 GAGCGG DrdI 2663 GACTCCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3071 GACGAGATCGTC EagI 294 CGGCCG C^GGCCG 3386 CGGCCG Eam1105I 148 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3551 GACAGCAAGTC EclXI 294 CGGCCG C^GGCCG 3386 CGGCCG Eco47III 555 AGCGCT AGC^GCT 3063 AGCGCT Eco52I 294 CGGCCG C^GGCCG 3386 CGGCCG MseI 1533 TTAA T^TAA 2275 TTAA PmaCI 1028 CACGTG CAC^GTG 3861 CACGTG PmlI 1028 CACGTG CAC^GTG 3861 CACGTG SacI 1132 GAGCTC GAGCT^C 1784 GAGCTC SmlI 710 CTCAAG C^T[CT][GA]AG 3908 CTTGAG Tru9I 1533 TTAA T^TAA 2275 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI AspHI AspI AvaI AvaII BalI BanI BanII BbeI BbvCI BbvI BcgI BcnI BfaI BglI BmyI BpmI BsaHI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI StyI TaqI TfiI TseI Tsp45I TspRI Tth111I TthHB8I Van91I XhoII XmaCI XmaI