##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII ApoI AscI Asp718I BciVI
BfrI BsiWI BsmI BspLU11I BsrDI
BssHII Bst1107I BstBI BstZ17I CpoI
DraI DraIII EarI EcoRI EcoRV
EcoT22I HindIII HpaI KpnI Ksp632I
MfeI MluI MunI NcoI NdeI
NheI NotI NsiI NspV PacI
PaeR7I PmeI PshAI Psp1406I PvuI
RsrII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 1037 TCCGGA T^CCGGA
AgeI 884 ACCGGT A^CCGGT
Alw44I 731 GTGCAC G^TGCAC
ApaLI 731 GTGCAC G^TGCAC
AseI 1532 ATTAAT AT^TAAT
AsnI 1532 ATTAAT AT^TAAT
Asp700I 1660 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2900 TGCGCA TGC^GCA
AvrII 1503 CCTAGG C^CTAGG
BamHI 2723 GGATCC G^GATCC
BbsI 997 GAAGAC GAAGAC(2/6)
BclI 2874 TGATCA T^GATCA
BglII 7 AGATCT A^GATCT
BlnI 1503 CCTAGG C^CTAGG
BlpI 1848 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1848 GCTGAGC GC^T[ACGT]AGC
BpuAI 997 GAAGAC GAAGAC(2/6)
BsaBI 1726 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1037 TCCGGA T^CCGGA
Bsp1407I 3327 TGTACA T^GTACA
BspDI 2837 ATCGAT AT^CGAT
BspEI 1037 TCCGGA T^CCGGA
BspHI 1703 TCATGA T^CATGA
BsrGI 3327 TGTACA T^GTACA
BssSI 3455 CACGAG CACGAG(-5/-1)
BstAPI 2974 GCATGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1848 GCTGAGC GC^T[ACGT]AGC
ClaI 2837 ATCGAT AT^CGAT
FbaI 2874 TGATCA T^GATCA
FseI 2515 GGCCGGCC GGCCGG^CC
FspI 2900 TGCGCA TGC^GCA
HincII 3803 GTCAAC GT[CT]^[GA]AC
HindII 3803 GTCAAC GT[CT]^[GA]AC
KspI 662 CCGCGG CCGC^GG
MamI 1726 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1037 TCCGGA T^CCGGA
NruI 3773 TCGCGA TCG^CGA
PinAI 884 ACCGGT A^CCGGT
PshBI 1532 ATTAAT AT^TAAT
RcaI 1703 TCATGA T^CATGA
SacII 662 CCGCGG CCGC^GG
ScaI 2820 AGTACT AGT^ACT
SphI 2974 GCATGC GCATG^C
SspBI 3327 TGTACA T^GTACA
StuI 1274 AGGCCT AGG^CCT
Tsp509I 3842 AATT ^AATT
XcmI 1604 CCAGGGCAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1660 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AhdI 148 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3551 GACAGCAAGTC
Aor51HI 555 AGCGCT AGC^GCT
3063 AGCGCT
AspEI 148 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3551 GACAGCAAGTC
BbrPI 1028 CACGTG CAC^GTG
3861 CACGTG
BsaAI 1028 CACGTG [CT]AC^GT[GA]
3861 CACGTG
BsgI 1887 CTGCAC GTGCAG(16/14)
3953 CTGCAC
BsiEI 294 CGGCCG CG[GA][CT]^CG
3386 CGGCCG
BsrBI 2705 CCGCTC CCGCTC(-3/-3)
1697 GAGCGG
DrdI 2663 GACTCCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3071 GACGAGATCGTC
EagI 294 CGGCCG C^GGCCG
3386 CGGCCG
Eam1105I 148 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3551 GACAGCAAGTC
EclXI 294 CGGCCG C^GGCCG
3386 CGGCCG
Eco47III 555 AGCGCT AGC^GCT
3063 AGCGCT
Eco52I 294 CGGCCG C^GGCCG
3386 CGGCCG
MseI 1533 TTAA T^TAA
2275 TTAA
PmaCI 1028 CACGTG CAC^GTG
3861 CACGTG
PmlI 1028 CACGTG CAC^GTG
3861 CACGTG
SacI 1132 GAGCTC GAGCT^C
1784 GAGCTC
SmlI 710 CTCAAG C^T[CT][GA]AG
3908 CTTGAG
Tru9I 1533 TTAA T^TAA
2275 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AspI AvaI AvaII BalI
BanI BanII BbeI BbvCI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BsaHI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI StyI TaqI
TfiI TseI Tsp45I TspRI Tth111I
TthHB8I Van91I XhoII XmaCI XmaI