##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflIII AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BbrPI BcgI
BciVI BclI BglII BlnI BlpI
Bpu1102I BsaBI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BssHII Bst1107I
BstAPI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DraI DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV FbaI FseI
FspI HgaI HincII HindII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NcoI NheI
NotI NruI PacI PaeR7I PflFI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SmaI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AflII 3298 CTTAAG C^TTAAG
AgeI 3554 ACCGGT A^CCGGT
AocI 3463 CCTGAGG CC^T[ACGT]AGG
ApaI 875 GGGCCC GGGCC^C
BalI 3011 TGGCCA TGG^CCA
BbvCI 714 CCTCAGC CCTCAGC(-5/-2)
BfrI 3298 CTTAAG C^TTAAG
BsaAI 2738 TACGTA [CT]AC^GT[GA]
BsmFI 3808 GTCCC GGGAC(10/14)
BsrBI 2660 CCGCTC CCGCTC(-3/-3)
BssSI 2609 CACGAG CACGAG(-5/-1)
BstBI 1900 TTCGAA TT^CGAA
Bsu36I 3463 CCTGAGG CC^T[ACGT]AGG
DrdI 3584 GACAGGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 3011 TGGCCA [CT]^GGCC[GA]
Eco81I 3463 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1416 ATGCAT ATGCA^T
MluNI 3011 TGGCCA TGG^CCA
MscI 3011 TGGCCA TGG^CCA
NaeI 355 GCCGGC GCC^GGC
NgoMIV 355 GCCGGC G^CCGGC
NsiI 1416 ATGCAT ATGCA^T
NspI 867 GCATGT [GA]CATG^[CT]
NspV 1900 TTCGAA TT^CGAA
PinAI 3554 ACCGGT A^CCGGT
PpuMI 1051 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2901 GACTTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 531 CTGCAG CTGCA^G
PvuII 658 CAGCTG CAG^CTG
SfuI 1900 TTCGAA TT^CGAA
SgrAI 375 CGCCGGTG C[GA]^CCGG[CT]G
SnaBI 2738 TACGTA TAC^GTA
StuI 1574 AGGCCT AGG^CCT
XcmI 323 CCATGAGACATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 3712 GTCTAC GT^[AC][GT]AC
3772 GTAGAC
AcyI 44 GGCGCC G[GA]^CG[CT]C
1594 GGCGCC
AlwNI 411 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
438 CAGTTTCTG
AseI 779 ATTAAT AT^TAAT
3916 ATTAAT
AsnI 779 ATTAAT AT^TAAT
3916 ATTAAT
BanI 44 GGCGCC G^G[CT][GA]CC
1594 GGCGCC
BbeI 44 GGCGCC GGCGC^C
1594 GGCGCC
BcnI 3402 CCGGG CC^[GC]GG
3536 CCGGG
BglI 5 GCCGGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
47 GCCGAGGCGGC
BsaHI 44 GGCGCC G[GA]^CG[CT]C
1594 GGCGCC
BsaWI 2442 ACCGGA [AT]^CCGG[AT]
3554 ACCGGT
BsrDI 3641 CATTGC GCAATG(2/0)
2632 GCAATG
BstXI 485 CCATCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3811 CCAGGACTTTGG
CfoI 45 GCGC GCG^C
1595 GCGC
EcoT14I 263 CCAAGG C^C[AT][AT]GG
403 CCAAGG
FauI 671 CCCGC CCCGC(4/6)
80 GCGGG
HaeII 44 GGCGCC [GA]GCGC^[CT]
1594 GGCGCC
HhaI 45 GCGC GCG^C
1595 GCGC
Hin1I 44 GGCGCC G[GA]^CG[CT]C
1594 GGCGCC
HinP1I 45 GCGC G^CGC
1595 GCGC
HindIII 739 AAGCTT A^AGCTT
1723 AAGCTT
KasI 44 GGCGCC G^GCGCC
1594 GGCGCC
MaeII 317 ACGT A^CGT
2739 ACGT
MspA1I 78 CAGCGG C[AC]G^C[GT]G
658 CAGCTG
NarI 44 GGCGCC GG^CGCC
1594 GGCGCC
NciI 3402 CCGGG CC^[GC]GG
3536 CCGGG
NdeI 2287 CATATG CA^TATG
3267 CATATG
PflMI 596 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
812 CCAGGAGTTGG
PshBI 779 ATTAAT AT^TAAT
3916 ATTAAT
SfoI 44 GGCGCC GGC^GCC
1594 GGCGCC
SmlI 3298 CTTAAG C^T[CT][GA]AG
3844 CTTGAG
StyI 263 CCAAGG C^C[AT][AT]GG
403 CCAAGG
Van91I 596 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
812 CCAGGAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BamHI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmI Bsp1286I
Bsp1407I BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr10I
Cfr13I DdeI DpnII DraII EarI
EcoNI EcoO109I EcoRI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI