##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII BanI
BbeI BbrPI BbvCI BclI BglI
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MluI MroI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 12 CGCG CG^CG
AocI 260 CCTGAGG CC^T[ACGT]AGG
AvaI 1317 CCCGAG C^[CT]CG[GA]G
BcgI 946 GCAGGAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1585 CCGGG CC^[GC]GG
BglII 249 AGATCT A^GATCT
BlpI 588 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 588 GCTCAGC GC^T[ACGT]AGC
BsaI 2938 GAGACC GGTCTC(1/5)
BsgI 1197 CTGCAC GTGCAG(16/14)
BsmI 901 GCATTC GAATGC(1/-1)
BsoBI 1317 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1088 TGTACA T^GTACA
BsrBI 2745 CCGCTC CCGCTC(-3/-3)
BsrGI 1088 TGTACA T^GTACA
BstUI 12 CGCG CG^CG
Bsu36I 260 CCTGAGG CC^T[ACGT]AGG
CelII 588 GCTCAGC GC^T[ACGT]AGC
DraIII 3443 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 260 CCTGAGG CC^T[ACGT]AGG
EcoRI 624 GAATTC G^AATTC
EcoT22I 2082 ATGCAT ATGCA^T
FauI 13 GCGGG CCCGC(4/6)
HindIII 2480 AAGCTT A^AGCTT
MfeI 3888 CAATTG C^AATTG
MunI 3888 CAATTG C^AATTG
MvnI 12 CGCG CG^CG
NciI 1585 CCGGG CC^[GC]GG
NcoI 87 CCATGG C^CATGG
NdeI 2281 CATATG CA^TATG
NsiI 2082 ATGCAT ATGCA^T
PflMI 2942 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 2910 CAGCTG CAG^CTG
SapI 3984 GAAGAGC GCTCTTC(1/4)
SexAI 313 ACCAGGT A^CC[AT]GGT
SspBI 1088 TGTACA T^GTACA
StuI 1732 AGGCCT AGG^CCT
Van91I 2942 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 920 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 2029 CTTAAG C^TTAAG
3472 CTTAAG
AlwNI 381 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2785 CAGGAGCTG
AvrII 1591 CCTAGG C^CTAGG
3614 CCTAGG
BalI 417 TGGCCA TGG^CCA
1267 TGGCCA
BciVI 80 GTATCC GTATCC(6/5)
508 GTATCC
BfrI 2029 CTTAAG C^TTAAG
3472 CTTAAG
BlnI 1591 CCTAGG C^CTAGG
3614 CCTAGG
BsaWI 308 ACCGGA [AT]^CCGG[AT]
933 ACCGGA
BssSI 522 CACGAG CACGAG(-5/-1)
2640 CTCGTG
CfoI 11 GCGC GCG^C
3255 GCGC
DraI 2199 TTTAAA TTT^AAA
3639 TTTAAA
DsaI 87 CCATGG C^C[GA][CT]GG
1756 CCGTGG
HhaI 11 GCGC GCG^C
3255 GCGC
HinP1I 11 GCGC G^CGC
3255 GCGC
MaeII 1764 ACGT A^CGT
3743 ACGT
MluNI 417 TGGCCA TGG^CCA
1267 TGGCCA
MscI 417 TGGCCA TGG^CCA
1267 TGGCCA
MspA1I 443 CAGCGG C[AC]G^C[GT]G
2910 CAGCTG
SspI 353 AATATT AAT^ATT
3702 AATATT
TaqI 955 TCGA T^CGA
1136 TCGA
TthHB8I 955 TCGA T^CGA
1136 TCGA
XcmI 84 CCACCATGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1065 CCAACGAGCAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaII
BamHI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspLU11I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI SacI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI