##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AgeI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AviII BamHI
BbeI BglII BsaHI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DrdI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI Hin1I HpaI KasI KpnI
MfeI MluI MroI MunI NarI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PpuMI
PshAI PshBI PvuI RcaI RsrII
SalI SapI SexAI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AclI 2583 AACGTT AA^CGTT
AflII 182 CTTAAG C^TTAAG
AflIII 978 ACATGT A^C[GA][CT]GT
AhdI 988 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1231 AGCGCT AGC^GCT
AspEI 988 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2602 CACGTG CAC^GTG
BcgI 3058 GCAGACCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 26 GGATAC GTATCC(6/5)
BfrI 182 CTTAAG C^TTAAG
BlpI 947 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 947 GCTGAGC GC^T[ACGT]AGC
BsaAI 2602 CACGTG [CT]AC^GT[GA]
BsaBI 1517 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1016 TCCGGT [AT]^CCGG[AT]
BspLU11I 978 ACATGT A^CATGT
BssSI 1104 CTCGTG CACGAG(-5/-1)
CelII 947 GCTGAGC GC^T[ACGT]AGC
DraI 1771 TTTAAA TTT^AAA
DraIII 3377 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 622 CGGCCG C^GGCCG
Eam1105I 988 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 622 CGGCCG C^GGCCG
Eco47III 1231 AGCGCT AGC^GCT
Eco52I 622 CGGCCG C^GGCCG
HaeII 1231 AGCGCT [GA]GCGC^[CT]
KspI 3211 CCGCGG CCGC^GG
MamI 1517 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 3294 GCCGGC GCC^GGC
NdeI 1622 CATATG CA^TATG
NgoMIV 3294 GCCGGC G^CCGGC
PflMI 3511 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2602 CACGTG CAC^GTG
PmlI 2602 CACGTG CAC^GTG
Psp1406I 2583 AACGTT AA^CGTT
SacI 4147 GAGCTC GAGCT^C
SacII 3211 CCGCGG CCGC^GG
SbfI 1008 CCTGCAGG CCTGCA^GG
ScaI 4242 AGTACT AGT^ACT
SmaI 3832 CCCGGG CCC^GGG
Sse8387I 1008 CCTGCAGG CCTGCA^GG
TaqI 3354 TCGA T^CGA
TfiI 1970 GAATC G^A[AT]TC
TthHB8I 3354 TCGA T^CGA
Van91I 3511 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1096 CCAAGGGCCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3832 CCCGGG C^CCGGG
XmaI 3832 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 1774 AAATTT [GA]^AATT[CT]
3356 GAATTT
ApoI 1774 AAATTT [GA]^AATT[CT]
3356 GAATTT
AspI 1801 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4022 GACTGTGTC
AvrII 1708 CCTAGG C^CTAGG
2312 CCTAGG
BbsI 2221 GTCTTC GAAGAC(2/6)
2749 GTCTTC
BlnI 1708 CCTAGG C^CTAGG
2312 CCTAGG
BpuAI 2221 GTCTTC GAAGAC(2/6)
2749 GTCTTC
BsiEI 622 CGGCCG CG[GA][CT]^CG
3043 CGACCG
BsmBI 1799 GAGACG CGTCTC(1/5)
3907 GAGACG
BsrDI 3968 CATTGC GCAATG(2/0)
4051 CATTGC
BsrFI 3045 ACCGGC [GA]^CCGG[CT]
3294 GCCGGC
BstAPI 3112 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3266 GCAGGATTTGC
Cfr10I 3045 ACCGGC [GA]^CCGG[CT]
3294 GCCGGC
PflFI 1801 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4022 GACTGTGTC
SfiI 1101 GGCCTCGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2990 GGCCTTCAGGGCC
Tth111I 1801 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4022 GACTGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BclI BcnI BfaI BglI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII