##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BbrPI BfrI BlnI BsaWI
BseAI BseRI BsiEI BsiWI BsmI
BspDI BspEI BsrBI BsrDI Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI FseI FspI HpaI KpnI
KspI MluI MroI NaeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SbfI SfiI SfuI SgrAI SpeI
Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AcyI 476 GGCGCC G[GA]^CG[CT]C
Alw44I 1990 GTGCAC G^TGCAC
ApaLI 1990 GTGCAC G^TGCAC
Asp700I 1773 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1940 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 927 TGGCCA TGG^CCA
BbeI 476 GGCGCC GGCGC^C
BbvCI 2510 CCTCAGC CCTCAGC(-5/-2)
BcgI 109 GCAGGGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3951 GTATCC GTATCC(6/5)
BlpI 4062 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4062 GCTCAGC GC^T[ACGT]AGC
BsaBI 680 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 476 GGCGCC G[GA]^CG[CT]C
BsaI 660 GGTCTC GGTCTC(1/5)
BsmBI 2233 CGTCTC CGTCTC(1/5)
Bsp1407I 3931 TGTACA T^GTACA
BsrFI 405 ACCGGC [GA]^CCGG[CT]
BsrGI 3931 TGTACA T^GTACA
BssSI 3467 CTCGTG CACGAG(-5/-1)
BstEII 691 GGTGACC G^GT[ACGT]ACC
BstPI 691 GGTGACC G^GT[ACGT]ACC
CelII 4062 GCTCAGC GC^T[ACGT]AGC
Cfr10I 405 ACCGGC [GA]^CCGG[CT]
DraIII 931 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1568 CCATGG C^C[GA][CT]GG
EcoO65I 691 GGTGACC G^GT[ACGT]ACC
EcoRI 671 GAATTC G^AATTC
EcoRV 2533 GATATC GAT^ATC
Hin1I 476 GGCGCC G[GA]^CG[CT]C
KasI 476 GGCGCC G^GCGCC
MamI 680 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2577 CAATTG C^AATTG
MluNI 927 TGGCCA TGG^CCA
MscI 927 TGGCCA TGG^CCA
MunI 2577 CAATTG C^AATTG
NarI 476 GGCGCC GG^CGCC
NcoI 1568 CCATGG C^CATGG
NdeI 608 CATATG CA^TATG
PaeR7I 4032 CTCGAG C^TCGAG
PflFI 1940 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 2107 CTGCAG CTGCA^G
SalI 1223 GTCGAC G^TCGAC
ScaI 65 AGTACT AGT^ACT
SexAI 1465 ACCTGGT A^CC[AT]GGT
SfoI 476 GGCGCC GGC^GCC
SmaI 3341 CCCGGG CCC^GGG
SnaBI 1958 TACGTA TAC^GTA
SspBI 3931 TGTACA T^GTACA
Tth111I 1940 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4032 CTCGAG C^TCGAG
XmaCI 3341 CCCGGG C^CCGGG
XmaI 3341 CCCGGG C^CCGGG
XmnI 1773 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1223 GTCGAC GT^[AC][GT]AC
1874 GTAGAC
AocI 3071 CCTCAGG CC^T[ACGT]AGG
4078 CCTGAGG
ApaI 335 GGGCCC GGGCC^C
2166 GGGCCC
BclI 1348 TGATCA T^GATCA
1667 TGATCA
BspHI 2610 TCATGA T^CATGA
3691 TCATGA
BspLU11I 3256 ACATGT A^CATGT
3524 ACATGT
BssHII 219 GCGCGC G^CGCGC
221 GCGCGC
BstAPI 1078 GCAGATTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2397 GCACGTCTTGC
Bsu36I 3071 CCTCAGG CC^T[ACGT]AGG
4078 CCTGAGG
Eco81I 3071 CCTCAGG CC^T[ACGT]AGG
4078 CCTGAGG
EcoT14I 1568 CCATGG C^C[AT][AT]GG
3221 CCAAGG
EcoT22I 971 ATGCAT ATGCA^T
1009 ATGCAT
FbaI 1348 TGATCA T^GATCA
1667 TGATCA
HaeII 138 AGCGCC [GA]GCGC^[CT]
476 GGCGCC
HindIII 740 AAGCTT A^AGCTT
3919 AAGCTT
NsiI 971 ATGCAT ATGCA^T
1009 ATGCAT
PflMI 697 CCATCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
930 CCACTAGGTGG
RcaI 2610 TCATGA T^CATGA
3691 TCATGA
SacI 3583 GAGCTC GAGCT^C
3595 GAGCTC
SphI 2415 GCATGC GCATG^C
3553 GCATGC
StuI 1507 AGGCCT AGG^CCT
3385 AGGCCT
StyI 1568 CCATGG C^C[AT][AT]GG
3221 CCAAGG
Van91I 697 CCATCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
930 CCACTAGGTGG
XcmI 1856 CCACCTTGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4097 CCACCACCTGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII