##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI AvrII BamHI BbrPI BcgI
BciVI BfrI BlnI BseAI BsiWI
BspDI BspEI BspHI BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI Eam1105I Eco47III EcoNI EcoRI
EcoRV FseI HincII HindII HindIII
HpaI MluI MroI NaeI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 2272 GACGTC GACGT^C
Acc65I 210 GGTACC G^GTACC
Alw44I 3478 GTGCAC G^TGCAC
ApaLI 3478 GTGCAC G^TGCAC
Asp700I 415 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 210 GGTACC G^GTACC
AspI 2646 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2591 TGCGCA TGC^GCA
BglII 1226 AGATCT A^GATCT
BsaAI 3617 TACGTG [CT]AC^GT[GA]
BsaBI 3999 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 3606 GCATTC GAATGC(1/-1)
BspMI 3966 GCAGGT ACCTGC(4/8)
BstEII 1972 GGTTACC G^GT[ACGT]ACC
BstPI 1972 GGTTACC G^GT[ACGT]ACC
BstXI 770 CCACGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 4082 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1972 GGTTACC G^GT[ACGT]ACC
EcoT22I 3499 ATGCAT ATGCA^T
FspI 2591 TGCGCA TGC^GCA
KpnI 210 GGTACC GGTAC^C
KspI 590 CCGCGG CCGC^GG
MamI 3999 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1215 CAATTG C^AATTG
MunI 1215 CAATTG C^AATTG
NdeI 3810 CATATG CA^TATG
NsiI 3499 ATGCAT ATGCA^T
PflFI 2646 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1752 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 590 CCGCGG CCGC^GG
SexAI 231 ACCAGGT A^CC[AT]GGT
SphI 3687 GCATGC GCATG^C
StuI 578 AGGCCT AGG^CCT
Tth111I 2646 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 415 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 1354 GAATTT [GA]^AATT[CT]
3413 GAATTT
AlwNI 364 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1750 CAGACCCTG
AocI 1393 CCTGAGG CC^T[ACGT]AGG
2257 CCTAAGG
ApaI 70 GGGCCC GGGCC^C
308 GGGCCC
ApoI 1354 GAATTT [GA]^AATT[CT]
3413 GAATTT
BbeI 106 GGCGCC GGCGC^C
2795 GGCGCC
BbvCI 3192 CCTCAGC CCTCAGC(-5/-2)
781 GCTGAGG
BclI 2421 TGATCA T^GATCA
2600 TGATCA
BglI 123 GCCCCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1438 GCCATCTGGGC
BlpI 2865 GCTTAGC GC^T[ACGT]AGC
3488 GCTCAGC
Bpu1102I 2865 GCTTAGC GC^T[ACGT]AGC
3488 GCTCAGC
BsaWI 471 TCCGGT [AT]^CCGG[AT]
2225 ACCGGA
BsrFI 848 ACCGGC [GA]^CCGG[CT]
2861 ACCGGC
BssHII 76 GCGCGC G^CGCGC
522 GCGCGC
BstAPI 2515 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3251 GCAACCCCTGC
Bsu36I 1393 CCTGAGG CC^T[ACGT]AGG
2257 CCTAAGG
CelII 2865 GCTTAGC GC^T[ACGT]AGC
3488 GCTCAGC
Cfr10I 848 ACCGGC [GA]^CCGG[CT]
2861 ACCGGC
DsaI 590 CCGCGG C^C[GA][CT]GG
3246 CCACGG
EagI 26 CGGCCG C^GGCCG
1485 CGGCCG
EclXI 26 CGGCCG C^GGCCG
1485 CGGCCG
Eco52I 26 CGGCCG C^GGCCG
1485 CGGCCG
Eco81I 1393 CCTGAGG CC^T[ACGT]AGG
2257 CCTAAGG
FbaI 2421 TGATCA T^GATCA
2600 TGATCA
HgaI 1629 GACGC GACGC(5/10)
1164 GCGTC
KasI 106 GGCGCC G^GCGCC
2795 GGCGCC
NarI 106 GGCGCC GG^CGCC
2795 GGCGCC
PflMI 1749 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2069 CCAAGATTTGG
PstI 756 CTGCAG CTGCA^G
3258 CTGCAG
SacI 649 GAGCTC GAGCT^C
3431 GAGCTC
SfcI 756 CTGCAG C^T[GA][CT]AG
3258 CTGCAG
SfoI 106 GGCGCC GGC^GCC
2795 GGCGCC
Van91I 1749 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2069 CCAAGATTTGG
XcmI 1439 CCATCTGGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3849 CCAATTCATGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspLU11I BsrBI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SmaI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI