##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AvaI
AviII BamHI BbrPI BciVI BcnI
BglI BglII BlpI Bpu1102I BsaAI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI FauI FseI FspI HindIII
HpaI KpnI KspI MaeII MfeI
MluI MunI NaeI NciI NgoMIV
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SgrAI SmaI
SnaBI Sse8387I SspBI StuI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccII 2833 CGCG CG^CG
AflII 1064 CTTAAG C^TTAAG
AspI 1399 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2523 CCTAGG C^CTAGG
BanII 2436 GAGCTC G[GA]GC[CT]^C
BbeI 2978 GGCGCC GGCGC^C
BcgI 155 GCAGTGGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2376 TGATCA T^GATCA
BfrI 1064 CTTAAG C^TTAAG
BlnI 2523 CCTAGG C^CTAGG
BstBI 1212 TTCGAA TT^CGAA
BstUI 2833 CGCG CG^CG
BstXI 2856 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2517 GGATCT [GA]^GATC[CT]
DrdI 215 GACCCTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1050 CCTATGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2376 TGATCA T^GATCA
KasI 2978 GGCGCC G^GCGCC
MflI 2517 GGATCT [GA]^GATC[CT]
MvnI 2833 CGCG CG^CG
NarI 2978 GGCGCC GG^CGCC
NheI 1423 GCTAGC G^CTAGC
NspV 1212 TTCGAA TT^CGAA
PflFI 1399 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1432 CCAATCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 752 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 2540 CAGCTG CAG^CTG
SacI 2436 GAGCTC GAGCT^C
SapI 629 GAAGAGC GCTCTTC(1/4)
SfoI 2978 GGCGCC GGC^GCC
SfuI 1212 TTCGAA TT^CGAA
SmlI 1064 CTTAAG C^T[CT][GA]AG
SpeI 3611 ACTAGT A^CTAGT
SphI 1427 GCATGC GCATG^C
Tth111I 1399 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1432 CCAATCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2062 TCTAGA T^CTAGA
XhoII 2517 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 169 TCCGGA T^CCGGA
2827 TCCGGA
AcyI 866 GGCGTC G[GA]^CG[CT]C
2978 GGCGCC
AflIII 721 ACATGT A^C[GA][CT]GT
1701 ACATGT
AocI 770 CCTCAGG CC^T[ACGT]AGG
2889 CCTGAGG
Asp700I 3220 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3573 GAAAGGATTC
BanI 2978 GGCGCC G^G[CT][GA]CC
3494 GGCACC
BbsI 709 GAAGAC GAAGAC(2/6)
1610 GAAGAC
BbvCI 2871 GCTGAGG CCTCAGC(-5/-2)
3006 GCTGAGG
BpmI 3074 CTCCAG CTGGAG(16/14)
201 CTGGAG
BpuAI 709 GAAGAC GAAGAC(2/6)
1610 GAAGAC
BsaBI 428 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3282 GATCAAAATC
BsaHI 866 GGCGTC G[GA]^CG[CT]C
2978 GGCGCC
BsaI 254 GAGACC GGTCTC(1/5)
1716 GGTCTC
BsaWI 169 TCCGGA [AT]^CCGG[AT]
2827 TCCGGA
BseAI 169 TCCGGA T^CCGGA
2827 TCCGGA
BsmBI 141 CGTCTC CGTCTC(1/5)
2936 CGTCTC
BsmI 2446 GAATGC GAATGC(1/-1)
2309 GCATTC
BspEI 169 TCCGGA T^CCGGA
2827 TCCGGA
BspLU11I 721 ACATGT A^CATGT
1701 ACATGT
BspMI 750 GCAGGT ACCTGC(4/8)
2877 GCAGGT
Bsu36I 770 CCTCAGG CC^T[ACGT]AGG
2889 CCTGAGG
CfoI 964 GCGC GCG^C
2979 GCGC
DraII 752 AGGTCCT [GA]G^G[ACGT]CC[CT]
3422 AGGCCCT
Eco81I 770 CCTCAGG CC^T[ACGT]AGG
2889 CCTGAGG
EcoO109I 752 AGGTCCT [GA]G^G[ACGT]CC[CT]
3422 AGGCCCT
EcoRV 1007 GATATC GAT^ATC
1340 GATATC
HaeII 963 GGCGCT [GA]GCGC^[CT]
2978 GGCGCC
HapII 170 CCGG C^CGG
2828 CCGG
HgaI 2831 GACGC GACGC(5/10)
867 GCGTC
HhaI 964 GCGC GCG^C
2979 GCGC
Hin1I 866 GGCGTC G[GA]^CG[CT]C
2978 GGCGCC
HinP1I 964 GCGC G^CGC
2979 GCGC
HpaII 170 CCGG C^CGG
2828 CCGG
MamI 428 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3282 GATCAAAATC
MroI 169 TCCGGA T^CCGGA
2827 TCCGGA
MspA1I 86 CCGCTG C[AC]G^C[GT]G
2540 CAGCTG
MspI 170 CCGG C^CGG
2828 CCGG
PstI 1023 CTGCAG CTGCA^G
1392 CTGCAG
TaqI 1213 TCGA T^CGA
1631 TCGA
TthHB8I 1213 TCGA T^CGA
1631 TCGA
XcmI 1266 CCATTGTTTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1409 CCAAATGGAAAATGG
XmnI 3220 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3573 GAAAGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AspHI AvaII
BalI BbvI BfaI BmyI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MvaI
MwoI NcoI NdeI NdeII NlaIII
NlaIV NsiI NspI PleI PshBI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI