##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Aor51HI AseI AsnI
Asp718I AspEI AviII BalI BamHI
BbrPI BciVI BclI BfrI BlpI
Bpu1102I BsaAI BsaBI BsiWI BsmI
BspDI BspHI BspLU11I Bst1107I BstEII
BstPI BstZ17I CelII ClaI Eam1105I
Eco47III EcoO65I EcoRI EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MluNI MscI MunI NdeI NheI
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI SalI SbfI ScaI
SfiI SgrAI SnaBI Sse8387I SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 1042 TCCGGA T^CCGGA
Alw44I 522 GTGCAC G^TGCAC
ApaLI 522 GTGCAC G^TGCAC
AscI 60 GGCGCGCC GG^CGCGCC
AspI 735 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BglII 3308 AGATCT A^GATCT
BsaI 2182 GGTCTC GGTCTC(1/5)
BseAI 1042 TCCGGA T^CCGGA
BsiEI 240 CGGCCG CG[GA][CT]^CG
Bsp1407I 4133 TGTACA T^GTACA
BspEI 1042 TCCGGA T^CCGGA
BsrDI 749 CATTGC GCAATG(2/0)
BsrGI 4133 TGTACA T^GTACA
BssHII 61 GCGCGC G^CGCGC
BssSI 355 CACGAG CACGAG(-5/-1)
BstAPI 78 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3856 TTCGAA TT^CGAA
CpoI 3924 CGGACCG CG^G[AT]CCG
DraIII 1990 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4153 GACACCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 240 CGGCCG C^GGCCG
EclXI 240 CGGCCG C^GGCCG
Eco52I 240 CGGCCG C^GGCCG
EcoNI 3144 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3294 ATGCAT ATGCA^T
MroI 1042 TCCGGA T^CCGGA
NcoI 35 CCATGG C^CATGG
NsiI 3294 ATGCAT ATGCA^T
NspI 701 GCATGC [GA]CATG^[CT]
NspV 3856 TTCGAA TT^CGAA
PflFI 735 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3483 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 801 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 3924 CGGACCG CG^G[AT]CCG
SacI 253 GAGCTC GAGCT^C
SapI 225 GCTCTTC GCTCTTC(1/4)
SexAI 3458 ACCAGGT A^CC[AT]GGT
SfuI 3856 TTCGAA TT^CGAA
SmlI 217 CTCAAG C^T[CT][GA]AG
SpeI 1962 ACTAGT A^CTAGT
SphI 701 GCATGC GCATG^C
SspBI 4133 TGTACA T^GTACA
StuI 4200 AGGCCT AGG^CCT
Tth111I 735 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3483 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1577 GTAGAC GT^[AC][GT]AC
2404 GTAGAC
AcsI 3037 AAATTC [GA]^AATT[CT]
3873 GAATTT
AlwNI 79 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
2498 CAGGAACTG
AocI 1395 CCTGAGG CC^T[ACGT]AGG
1850 CCTAAGG
ApoI 3037 AAATTC [GA]^AATT[CT]
3873 GAATTT
Asp700I 438 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1303 GAACGGCTTC
AvrII 2260 CCTAGG C^CTAGG
2386 CCTAGG
BbeI 877 GGCGCC GGCGC^C
4218 GGCGCC
BbsI 363 GAAGAC GAAGAC(2/6)
3912 GAAGAC
BbvCI 3698 CCTCAGC CCTCAGC(-5/-2)
3427 GCTGAGG
BcgI 1239 GCAAGTGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4173 GCATCAAGTTCG
BlnI 2260 CCTAGG C^CTAGG
2386 CCTAGG
BpuAI 363 GAAGAC GAAGAC(2/6)
3912 GAAGAC
BspMI 2478 ACCTGC ACCTGC(4/8)
4017 GCAGGT
BstXI 650 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2924 CCACCTGTGTGG
Bsu36I 1395 CCTGAGG CC^T[ACGT]AGG
1850 CCTAAGG
DraI 2351 TTTAAA TTT^AAA
4256 TTTAAA
Eco81I 1395 CCTGAGG CC^T[ACGT]AGG
1850 CCTAAGG
KasI 877 GGCGCC G^GCGCC
4218 GGCGCC
MaeII 482 ACGT A^CGT
4160 ACGT
NaeI 14 GCCGGC GCC^GGC
65 GCCGGC
NarI 877 GGCGCC GG^CGCC
4218 GGCGCC
NgoMIV 14 GCCGGC G^CCGGC
65 GCCGGC
SfoI 877 GGCGCC GGC^GCC
4218 GGCGCC
XmnI 438 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1303 GAACGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI