##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BbrPI
BfrI BglII BlnI BlpI Bpu1102I
BseAI BsiWI BsmBI BspDI BspEI
BspLU11I BssSI Bst1107I BstBI BstZ17I
CelII ClaI DraI Eam1105I EcoRI
EcoRV EcoT22I HpaI KpnI MfeI
MluI MroI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI SbfI
SfuI SnaBI SpeI SphI Sse8387I
SspI SwaI Tru9I Tth111I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflIII 2352 ACGTGT A^C[GA][CT]GT
AgeI 223 ACCGGT A^CCGGT
Aor51HI 3185 AGCGCT AGC^GCT
AviII 999 TGCGCA TGC^GCA
BcgI 2737 CGACCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3411 GTATCC GTATCC(6/5)
BclI 2533 TGATCA T^GATCA
BsaBI 2863 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 321 GCATTC GAATGC(1/-1)
Bsp1407I 1581 TGTACA T^GTACA
BspHI 2958 TCATGA T^CATGA
BsrGI 1581 TGTACA T^GTACA
BstAPI 1596 GCAAGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 225 CGGTCCG CG^G[AT]CCG
DraIII 1635 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3185 AGCGCT AGC^GCT
FbaI 2533 TGATCA T^GATCA
FseI 3382 GGCCGGCC GGCCGG^CC
FspI 999 TGCGCA TGC^GCA
HindIII 4172 AAGCTT A^AGCTT
KspI 241 CCGCGG CCGC^GG
MamI 2863 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PinAI 223 ACCGGT A^CCGGT
RcaI 2958 TCATGA T^CATGA
RsrII 225 CGGTCCG CG^G[AT]CCG
SacI 788 GAGCTC GAGCT^C
SacII 241 CCGCGG CCGC^GG
SalI 2415 GTCGAC G^TCGAC
SapI 1942 GAAGAGC GCTCTTC(1/4)
ScaI 1932 AGTACT AGT^ACT
SfiI 4198 GGCCAGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 411 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1551 CCCGGG CCC^GGG
SspBI 1581 TGTACA T^GTACA
XcmI 638 CCAGAAAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1551 CCCGGG C^CCGGG
XmaI 1551 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 779 GTCTAC GT^[AC][GT]AC
2415 GTCGAC
AcsI 2393 GAATTT [GA]^AATT[CT]
3116 AAATTC
ApoI 2393 GAATTT [GA]^AATT[CT]
3116 AAATTC
BalI 468 TGGCCA TGG^CCA
2451 TGGCCA
BbeI 203 GGCGCC GGCGC^C
1055 GGCGCC
BbsI 3353 GAAGAC GAAGAC(2/6)
2171 GTCTTC
BfaI 450 CTAG C^TAG
2919 CTAG
BpuAI 3353 GAAGAC GAAGAC(2/6)
2171 GTCTTC
BsaWI 223 ACCGGT [AT]^CCGG[AT]
3099 ACCGGA
BsiEI 979 CGGCCG CG[GA][CT]^CG
1791 CGGCCG
BsrBI 33 GAGCGG CCGCTC(-3/-3)
1895 GAGCGG
BstEII 2566 GGTCACC G^GT[ACGT]ACC
3373 GGTCACC
BstPI 2566 GGTCACC G^GT[ACGT]ACC
3373 GGTCACC
EagI 979 CGGCCG C^GGCCG
1791 CGGCCG
EclXI 979 CGGCCG C^GGCCG
1791 CGGCCG
Eco52I 979 CGGCCG C^GGCCG
1791 CGGCCG
EcoNI 2503 CCTAAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3867 CCTCCCTCAGG
EcoO65I 2566 GGTCACC G^GT[ACGT]ACC
3373 GGTCACC
KasI 203 GGCGCC G^GCGCC
1055 GGCGCC
MaeI 450 CTAG C^TAG
2919 CTAG
MluNI 468 TGGCCA TGG^CCA
2451 TGGCCA
MscI 468 TGGCCA TGG^CCA
2451 TGGCCA
NarI 203 GGCGCC GG^CGCC
1055 GGCGCC
PaeR7I 1535 CTCGAG C^TCGAG
2555 CTCGAG
SfoI 203 GGCGCC GGC^GCC
1055 GGCGCC
StuI 2511 AGGCCT AGG^CCT
3610 AGGCCT
XhoI 1535 CTCGAG C^TCGAG
2555 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BamHI
BanI BanII BbvCI BbvI BcnI
BglI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII