################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AccIII AclI AhdI Alw44I ApaLI AscI AseI AsnI AspEI AspI AvrII BamHI BclI BglII BlnI BsaBI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII Bst1107I BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoRV FbaI FseI HpaI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NotI NruI PacI PaeR7I PflFI PmeI PshBI Psp1406I PvuI RsrII SbfI ScaI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 1981 GACGTC GACGT^C Acc65I 914 GGTACC G^GTACC AflII 2623 CTTAAG C^TTAAG AgeI 911 ACCGGT A^CCGGT Aor51HI 1454 AGCGCT AGC^GCT Asp718I 914 GGTACC G^GTACC AvaI 1590 CTCGGG C^[CT]CG[GA]G AviII 2356 TGCGCA TGC^GCA BbeI 3775 GGCGCC GGCGC^C BciVI 3590 GGATAC GTATCC(6/5) BfrI 2623 CTTAAG C^TTAAG BglI 1243 GCCCGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 3738 GGTCTC GGTCTC(1/5) BsoBI 1590 CTCGGG C^[CT]CG[GA]G BspHI 3710 TCATGA T^CATGA BsrFI 911 ACCGGT [GA]^CCGG[CT] BssSI 400 CACGAG CACGAG(-5/-1) BstAPI 962 GCATCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 193 TTCGAA TT^CGAA BstEII 3179 GGTGACC G^GT[ACGT]ACC BstPI 3179 GGTGACC G^GT[ACGT]ACC Cfr10I 911 ACCGGT [GA]^CCGG[CT] DrdI 588 GACAGATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1454 AGCGCT AGC^GCT EcoNI 519 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3179 GGTGACC G^GT[ACGT]ACC EcoRI 3906 GAATTC G^AATTC EcoT22I 2205 ATGCAT ATGCA^T FspI 2356 TGCGCA TGC^GCA HincII 3976 GTCGAC GT[CT]^[GA]AC HindII 3976 GTCGAC GT[CT]^[GA]AC HindIII 2549 AAGCTT A^AGCTT KasI 3775 GGCGCC G^GCGCC KpnI 914 GGTACC GGTAC^C NarI 3775 GGCGCC GG^CGCC NheI 3140 GCTAGC G^CTAGC NsiI 2205 ATGCAT ATGCA^T NspI 3995 GCATGT [GA]CATG^[CT] NspV 193 TTCGAA TT^CGAA PinAI 911 ACCGGT A^CCGGT PshAI 1149 GACGCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 3710 TCATGA T^CATGA SalI 3976 GTCGAC G^TCGAC SexAI 1061 ACCTGGT A^CC[AT]GGT SfoI 3775 GGCGCC GGC^GCC SfuI 193 TTCGAA TT^CGAA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AflIII 2631 ACGTGT A^C[GA][CT]GT 2851 ACACGT AocI 408 CCTCAGG CC^T[ACGT]AGG 1991 CCTCAGG ApaI 2051 GGGCCC GGGCC^C 3253 GGGCCC Asp700I 864 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1377 GAAGTGCTTC BbrPI 2630 CACGTG CAC^GTG 2852 CACGTG BlpI 307 GCTGAGC GC^T[ACGT]AGC 1485 GCTGAGC Bpu1102I 307 GCTGAGC GC^T[ACGT]AGC 1485 GCTGAGC BsaAI 2630 CACGTG [CT]AC^GT[GA] 2852 CACGTG BspMI 917 ACCTGC ACCTGC(4/8) 3061 ACCTGC BsrDI 1837 CATTGC GCAATG(2/0) 4032 GCAATG Bsu36I 408 CCTCAGG CC^T[ACGT]AGG 1991 CCTCAGG CelII 307 GCTGAGC GC^T[ACGT]AGC 1485 GCTGAGC DraIII 2497 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG 2499 CACTGTGTG Eco81I 408 CCTCAGG CC^T[ACGT]AGG 1991 CCTCAGG PflMI 951 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3948 CCAAGCGTTGG PmaCI 2630 CACGTG CAC^GTG 2852 CACGTG PmlI 2630 CACGTG CAC^GTG 2852 CACGTG PstI 427 CTGCAG CTGCA^G 3236 CTGCAG Van91I 951 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3948 CCAAGCGTTGG XcmI 2024 CCACAAGCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2559 CCATGTGTGTCCTGG XmnI 864 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1377 GAAGTGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BalI BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI SacI SacII SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII