##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AccIII AclI AhdI Alw44I ApaLI
AscI AseI AsnI AspEI AspI
AvrII BamHI BclI BglII BlnI
BsaBI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII Bst1107I BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
EcoRV FbaI FseI HpaI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NotI NruI PacI
PaeR7I PflFI PmeI PshBI Psp1406I
PvuI RsrII SbfI ScaI SfiI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1981 GACGTC GACGT^C
Acc65I 914 GGTACC G^GTACC
AflII 2623 CTTAAG C^TTAAG
AgeI 911 ACCGGT A^CCGGT
Aor51HI 1454 AGCGCT AGC^GCT
Asp718I 914 GGTACC G^GTACC
AvaI 1590 CTCGGG C^[CT]CG[GA]G
AviII 2356 TGCGCA TGC^GCA
BbeI 3775 GGCGCC GGCGC^C
BciVI 3590 GGATAC GTATCC(6/5)
BfrI 2623 CTTAAG C^TTAAG
BglI 1243 GCCCGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 3738 GGTCTC GGTCTC(1/5)
BsoBI 1590 CTCGGG C^[CT]CG[GA]G
BspHI 3710 TCATGA T^CATGA
BsrFI 911 ACCGGT [GA]^CCGG[CT]
BssSI 400 CACGAG CACGAG(-5/-1)
BstAPI 962 GCATCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 193 TTCGAA TT^CGAA
BstEII 3179 GGTGACC G^GT[ACGT]ACC
BstPI 3179 GGTGACC G^GT[ACGT]ACC
Cfr10I 911 ACCGGT [GA]^CCGG[CT]
DrdI 588 GACAGATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1454 AGCGCT AGC^GCT
EcoNI 519 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3179 GGTGACC G^GT[ACGT]ACC
EcoRI 3906 GAATTC G^AATTC
EcoT22I 2205 ATGCAT ATGCA^T
FspI 2356 TGCGCA TGC^GCA
HincII 3976 GTCGAC GT[CT]^[GA]AC
HindII 3976 GTCGAC GT[CT]^[GA]AC
HindIII 2549 AAGCTT A^AGCTT
KasI 3775 GGCGCC G^GCGCC
KpnI 914 GGTACC GGTAC^C
NarI 3775 GGCGCC GG^CGCC
NheI 3140 GCTAGC G^CTAGC
NsiI 2205 ATGCAT ATGCA^T
NspI 3995 GCATGT [GA]CATG^[CT]
NspV 193 TTCGAA TT^CGAA
PinAI 911 ACCGGT A^CCGGT
PshAI 1149 GACGCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3710 TCATGA T^CATGA
SalI 3976 GTCGAC G^TCGAC
SexAI 1061 ACCTGGT A^CC[AT]GGT
SfoI 3775 GGCGCC GGC^GCC
SfuI 193 TTCGAA TT^CGAA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 2631 ACGTGT A^C[GA][CT]GT
2851 ACACGT
AocI 408 CCTCAGG CC^T[ACGT]AGG
1991 CCTCAGG
ApaI 2051 GGGCCC GGGCC^C
3253 GGGCCC
Asp700I 864 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1377 GAAGTGCTTC
BbrPI 2630 CACGTG CAC^GTG
2852 CACGTG
BlpI 307 GCTGAGC GC^T[ACGT]AGC
1485 GCTGAGC
Bpu1102I 307 GCTGAGC GC^T[ACGT]AGC
1485 GCTGAGC
BsaAI 2630 CACGTG [CT]AC^GT[GA]
2852 CACGTG
BspMI 917 ACCTGC ACCTGC(4/8)
3061 ACCTGC
BsrDI 1837 CATTGC GCAATG(2/0)
4032 GCAATG
Bsu36I 408 CCTCAGG CC^T[ACGT]AGG
1991 CCTCAGG
CelII 307 GCTGAGC GC^T[ACGT]AGC
1485 GCTGAGC
DraIII 2497 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
2499 CACTGTGTG
Eco81I 408 CCTCAGG CC^T[ACGT]AGG
1991 CCTCAGG
PflMI 951 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3948 CCAAGCGTTGG
PmaCI 2630 CACGTG CAC^GTG
2852 CACGTG
PmlI 2630 CACGTG CAC^GTG
2852 CACGTG
PstI 427 CTGCAG CTGCA^G
3236 CTGCAG
Van91I 951 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3948 CCAAGCGTTGG
XcmI 2024 CCACAAGCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2559 CCATGTGTGTCCTGG
XmnI 864 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1377 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI SacI SacII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII