##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccI AccIII AflII AgeI
Alw44I Aor51HI ApaLI AscI Asp718I
AviII AvrII BfrI BlnI BlpI
Bpu1102I BseAI BsiEI BsiWI BspEI
BspHI BsrFI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
Cfr10I CpoI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV FseI
FspI KpnI MluI MroI NaeI
NcoI NgoMIV NheI NotI NruI
NspV PacI PinAI PmeI PshAI
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 67 enzymes) #
###################################
AatII 1965 GACGTC GACGT^C
AclI 1732 AACGTT AA^CGTT
AflIII 3354 ACATGT A^C[GA][CT]GT
AhdI 3274 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 227 GGGCCC GGGCC^C
AspEI 3274 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2843 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 852 TGGCCA TGG^CCA
BamHI 192 GGATCC G^GATCC
BanI 58 GGCGCC G^G[CT][GA]CC
BbeI 58 GGCGCC GGCGC^C
BbrPI 700 CACGTG CAC^GTG
BbsI 3271 GAAGAC GAAGAC(2/6)
BcgI 407 CGAGGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3179 GTATCC GTATCC(6/5)
BclI 3419 TGATCA T^GATCA
BcnI 2036 CCCGG CC^[GC]GG
BglII 993 AGATCT A^GATCT
BpuAI 3271 GAAGAC GAAGAC(2/6)
BsaAI 700 CACGTG [CT]AC^GT[GA]
BsaBI 3665 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1357 ACCGGA [AT]^CCGG[AT]
BsgI 445 GTGCAG GTGCAG(16/14)
BsmBI 347 GAGACG CGTCTC(1/5)
Bsp1407I 542 TGTACA T^GTACA
BspDI 726 ATCGAT AT^CGAT
BspLU11I 3354 ACATGT A^CATGT
BspMI 286 ACCTGC ACCTGC(4/8)
BsrBI 91 CCGCTC CCGCTC(-3/-3)
BsrGI 542 TGTACA T^GTACA
BstAPI 383 GCAGGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 726 ATCGAT AT^CGAT
DraIII 1186 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2372 GACATATATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3026 CCGCGG C^C[GA][CT]GG
EaeI 852 TGGCCA [CT]^GGCC[GA]
Eam1105I 3274 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 639 CCTTGG C^C[AT][AT]GG
FauI 90 CCCGC CCCGC(4/6)
FbaI 3419 TGATCA T^GATCA
HaeII 58 GGCGCC [GA]GCGC^[CT]
HindIII 794 AAGCTT A^AGCTT
HpaI 2198 GTTAAC GTT^AAC
KasI 58 GGCGCC G^GCGCC
KspI 3026 CCGCGG CCGC^GG
MamI 3665 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1233 CAATTG C^AATTG
MluNI 852 TGGCCA TGG^CCA
MscI 852 TGGCCA TGG^CCA
MunI 1233 CAATTG C^AATTG
NarI 58 GGCGCC GG^CGCC
NciI 2036 CCCGG CC^[GC]GG
PaeR7I 495 CTCGAG C^TCGAG
PflFI 2843 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1185 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 700 CACGTG CAC^GTG
PmlI 700 CACGTG CAC^GTG
Psp1406I 1732 AACGTT AA^CGTT
PstI 486 CTGCAG CTGCA^G
SacII 3026 CCGCGG CCGC^GG
SfoI 58 GGCGCC GGC^GCC
SspBI 542 TGTACA T^GTACA
StyI 639 CCTTGG C^C[AT][AT]GG
Tth111I 2843 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1185 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 556 TCTAGA T^CTAGA
XhoI 495 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 43 CCTGAGG CC^T[ACGT]AGG
196 CCTAAGG
AseI 2744 ATTAAT AT^TAAT
3523 ATTAAT
AsnI 2744 ATTAAT AT^TAAT
3523 ATTAAT
AvaI 405 CCCGAG C^[CT]CG[GA]G
495 CTCGAG
BbvCI 3569 CCTCAGC CCTCAGC(-5/-2)
3043 GCTGAGG
BglI 845 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2552 GCCCTCTGGGC
BpmI 1410 CTCCAG CTGGAG(16/14)
1526 CTGGAG
BsmI 457 GAATGC GAATGC(1/-1)
3135 GCATTC
BsoBI 405 CCCGAG C^[CT]CG[GA]G
495 CTCGAG
BsrDI 2031 CATTGC GCAATG(2/0)
2142 GCAATG
Bsu36I 43 CCTGAGG CC^T[ACGT]AGG
196 CCTAAGG
Eco81I 43 CCTGAGG CC^T[ACGT]AGG
196 CCTAAGG
HgaI 147 GACGC GACGC(5/10)
66 GCGTC
MslI 1036 CATAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1270 CATACAGGTG
NdeI 1258 CATATG CA^TATG
1858 CATATG
PpuMI 1951 AGGTCCC [GA]G^G[AT]CC[CT]
2847 AGGTCCC
PshBI 2744 ATTAAT AT^TAAT
3523 ATTAAT
PvuII 1022 CAGCTG CAG^CTG
3441 CAGCTG
SphI 1337 GCATGC GCATG^C
1421 GCATGC
SspI 1816 AATATT AAT^ATT
4170 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BanII BbvI BfaI
BmyI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssHII BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRI EcoRII EcoT22I
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NsiI NspI PleI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI