##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII BbeI
BcgI BfrI BglII BseAI BsiWI
BsmI BspEI BstBI DrdI Eam1105I
EcoNI EcoT22I FseI FspI HindIII
KasI KpnI MfeI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PmeI PshAI
PshBI Psp1406I PvuI SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Alw44I 3755 GTGCAC G^TGCAC
AocI 3819 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2713 AGCGCT AGC^GCT
ApaLI 3755 GTGCAC G^TGCAC
AvrII 3783 CCTAGG C^CTAGG
BamHI 4131 GGATCC G^GATCC
BbrPI 2792 CACGTG CAC^GTG
BbvCI 3668 GCTGAGG CCTCAGC(-5/-2)
BciVI 2007 GGATAC GTATCC(6/5)
BclI 2114 TGATCA T^GATCA
BglI 576 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3783 CCTAGG C^CTAGG
BlpI 467 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 467 GCTCAGC GC^T[ACGT]AGC
BsaBI 2211 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 214 CGGCCG CG[GA][CT]^CG
BspDI 517 ATCGAT AT^CGAT
BspHI 4161 TCATGA T^CATGA
BspLU11I 3010 ACATGT A^CATGT
BssHII 13 GCGCGC G^CGCGC
BssSI 4136 CACGAG CACGAG(-5/-1)
Bst1107I 3002 GTATAC GTA^TAC
BstAPI 153 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 988 CCATCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3002 GTATAC GTA^TAC
Bsu36I 3819 CCTGAGG CC^T[ACGT]AGG
CelII 467 GCTCAGC GC^T[ACGT]AGC
ClaI 517 ATCGAT AT^CGAT
CpoI 295 CGGACCG CG^G[AT]CCG
DraIII 2792 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 214 CGGCCG C^GGCCG
EarI 1642 GAAGAG CTCTTC(1/4)
EclXI 214 CGGCCG C^GGCCG
Eco47III 2713 AGCGCT AGC^GCT
Eco52I 214 CGGCCG C^GGCCG
Eco81I 3819 CCTGAGG CC^T[ACGT]AGG
EcoRI 2967 GAATTC G^AATTC
FbaI 2114 TGATCA T^GATCA
HincII 2935 GTTAAC GT[CT]^[GA]AC
HindII 2935 GTTAAC GT[CT]^[GA]AC
HpaI 2935 GTTAAC GTT^AAC
Ksp632I 1642 GAAGAG CTCTTC(1/4)
KspI 292 CCGCGG CCGC^GG
MamI 2211 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 3365 GCTAGC G^CTAGC
PmaCI 2792 CACGTG CAC^GTG
PmlI 2792 CACGTG CAC^GTG
PpuMI 964 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 4161 TCATGA T^CATGA
RsrII 295 CGGACCG CG^G[AT]CCG
SacII 292 CCGCGG CCGC^GG
ScaI 2240 AGTACT AGT^ACT
SpeI 121 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1938 GTAGAC GT^[AC][GT]AC
3002 GTATAC
AgeI 298 ACCGGT A^CCGGT
556 ACCGGT
AvaI 799 CTCGGG C^[CT]CG[GA]G
1001 CTCGGG
BalI 817 TGGCCA TGG^CCA
1927 TGGCCA
BsaAI 1977 TACGTG [CT]AC^GT[GA]
2792 CACGTG
BsgI 117 CTGCAC GTGCAG(16/14)
3969 GTGCAG
BsmFI 3639 GGGAC GGGAC(10/14)
1238 GTCCC
BsoBI 799 CTCGGG C^[CT]CG[GA]G
1001 CTCGGG
BsrBI 2457 CCGCTC CCGCTC(-3/-3)
360 GAGCGG
BsrDI 1994 CATTGC GCAATG(2/0)
2880 GCAATG
BstEII 881 GGTCACC G^GT[ACGT]ACC
3766 GGTTACC
BstPI 881 GGTCACC G^GT[ACGT]ACC
3766 GGTTACC
DraI 2867 TTTAAA TTT^AAA
3856 TTTAAA
EcoO65I 881 GGTCACC G^GT[ACGT]ACC
3766 GGTTACC
EcoT14I 3783 CCTAGG C^C[AT][AT]GG
4111 CCAAGG
MluNI 817 TGGCCA TGG^CCA
1927 TGGCCA
MscI 817 TGGCCA TGG^CCA
1927 TGGCCA
NspI 1089 GCATGT [GA]CATG^[CT]
3010 ACATGT
PflMI 683 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2076 CCATTCCGTGG
PinAI 298 ACCGGT A^CCGGT
556 ACCGGT
SacI 1355 GAGCTC GAGCT^C
2184 GAGCTC
StuI 2990 AGGCCT AGG^CCT
3982 AGGCCT
StyI 3783 CCTAGG C^C[AT][AT]GG
4111 CCAAGG
Van91I 683 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2076 CCATTCCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI Bsp1286I Bsp1407I BspMI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoRV
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI