##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccI AccII AccIII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BcgI BglI
BlnI BlpI Bpu1102I BsaAI BsaWI
BseAI BsgI BsiEI BsiWI BspDI
BspEI BspLU11I BsrBI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
FseI FspI HgaI KpnI KspI
MluI MroI MvnI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PstI
PvuI RsrII SacII SalI SbfI
SfiI SfuI SgrAI SmaI SnaBI
Sse8387I StuI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 78 GACGTC GACGT^C
ApaI 21 GGGCCC GGGCC^C
AseI 2957 ATTAAT AT^TAAT
AsnI 2957 ATTAAT AT^TAAT
BalI 907 TGGCCA TGG^CCA
BbeI 96 GGCGCC GGCGC^C
BbvCI 1737 GCTGAGG CCTCAGC(-5/-2)
BciVI 3993 GGATAC GTATCC(6/5)
BclI 548 TGATCA T^GATCA
BglII 4316 AGATCT A^GATCT
BsaBI 4093 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 3255 GAGGAG GAGGAG(10/8)
BsmFI 184 GGGAC GGGAC(10/14)
Bsp1407I 4242 TGTACA T^GTACA
BsrFI 7 GCCGGC [GA]^CCGG[CT]
BsrGI 4242 TGTACA T^GTACA
BssSI 1666 CACGAG CACGAG(-5/-1)
BstXI 3310 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 7 GCCGGC [GA]^CCGG[CT]
DraIII 1228 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 548 TGATCA T^GATCA
HindIII 375 AAGCTT A^AGCTT
KasI 96 GGCGCC G^GCGCC
MamI 4093 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1543 CAATTG C^AATTG
MluNI 907 TGGCCA TGG^CCA
MscI 907 TGGCCA TGG^CCA
MunI 1543 CAATTG C^AATTG
NaeI 7 GCCGGC GCC^GGC
NarI 96 GGCGCC GG^CGCC
NcoI 757 CCATGG C^CATGG
NdeI 2657 CATATG CA^TATG
NgoMIV 7 GCCGGC G^CCGGC
NheI 1637 GCTAGC G^CTAGC
PflMI 1227 CCACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 184 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 2957 ATTAAT AT^TAAT
SapI 1353 GAAGAGC GCTCTTC(1/4)
SexAI 3814 ACCAGGT A^CC[AT]GGT
SfoI 96 GGCGCC GGC^GCC
SpeI 3973 ACTAGT A^CTAGT
SphI 3430 GCATGC GCATG^C
SspBI 4242 TGTACA T^GTACA
TaqI 401 TCGA T^CGA
TthHB8I 401 TCGA T^CGA
Van91I 1227 CCACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AclI 492 AACGTT AA^CGTT
4009 AACGTT
AcyI 78 GACGTC G[GA]^CG[CT]C
96 GGCGCC
AflII 474 CTTAAG C^TTAAG
3741 CTTAAG
AlwNI 1933 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
2149 CAGAATCTG
Asp700I 462 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1377 GAACTGGTTC
AvaI 1960 CTCGGG C^[CT]CG[GA]G
3451 CTCGGG
BfrI 474 CTTAAG C^TTAAG
3741 CTTAAG
BsaHI 78 GACGTC G[GA]^CG[CT]C
96 GGCGCC
BsaI 221 GAGACC GGTCTC(1/5)
3360 GAGACC
BsoBI 1960 CTCGGG C^[CT]CG[GA]G
3451 CTCGGG
BsrDI 3520 CATTGC GCAATG(2/0)
2351 GCAATG
HaeII 47 GGCGCT [GA]GCGC^[CT]
96 GGCGCC
Hin1I 78 GACGTC G[GA]^CG[CT]C
96 GGCGCC
HpaI 813 GTTAAC GTT^AAC
3858 GTTAAC
MslI 2833 CACTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2839 CATGGCTGTG
Psp1406I 492 AACGTT AA^CGTT
4009 AACGTT
SacI 155 GAGCTC GAGCT^C
582 GAGCTC
ScaI 3248 AGTACT AGT^ACT
4228 AGTACT
SfcI 541 CTGTAG C^T[GA][CT]AG
1415 CTGTAG
SspI 1108 AATATT AAT^ATT
2028 AATATT
XbaI 1349 TCTAGA T^CTAGA
2997 TCTAGA
XmnI 462 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1377 GAACTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AspHI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmI
Bsp1286I BspHI BspMI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PleI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII