##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspI AviII BbeI
BciVI BclI BfrI BsaHI BseAI
BsiEI BsiWI BspEI BspHI BsrDI
BssHII Bst1107I BstAPI BstBI BstZ17I
DsaI EagI EclXI Eco47III Eco52I
EcoNI EcoRI EcoT22I FbaI FseI
FspI Hin1I HpaI KasI KspI
MluI MroI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI SacII
SapI ScaI SexAI SfoI SfuI
SgrAI SnaBI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 1264 GGTACC G^GTACC
AccI 2051 GTCGAC GT^[AC][GT]AC
AhdI 2274 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 804 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1264 GGTACC G^GTACC
AspEI 2274 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4196 CCTAGG C^CTAGG
BamHI 3947 GGATCC G^GATCC
BbrPI 2867 CACGTG CAC^GTG
BcgI 1707 GCAGAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 4196 CCTAGG C^CTAGG
BlpI 2816 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2816 GCTCAGC GC^T[ACGT]AGC
BsaAI 2867 CACGTG [CT]AC^GT[GA]
BsaBI 1676 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 324 GAGACC GGTCTC(1/5)
BsaWI 3937 TCCGGT [AT]^CCGG[AT]
BsmBI 3126 GAGACG CGTCTC(1/5)
Bsp1407I 896 TGTACA T^GTACA
BspDI 1776 ATCGAT AT^CGAT
BsrFI 1127 GCCGGC [GA]^CCGG[CT]
BsrGI 896 TGTACA T^GTACA
CelII 2816 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1127 GCCGGC [GA]^CCGG[CT]
ClaI 1776 ATCGAT AT^CGAT
CpoI 1524 CGGACCG CG^G[AT]CCG
DraI 2995 TTTAAA TTT^AAA
Eam1105I 2274 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1287 GATATC GAT^ATC
HincII 2051 GTCGAC GT[CT]^[GA]AC
HindII 2051 GTCGAC GT[CT]^[GA]AC
KpnI 1264 GGTACC GGTAC^C
MamI 1676 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1127 GCCGGC GCC^GGC
NgoMIV 1127 GCCGGC G^CCGGC
PaeR7I 758 CTCGAG C^TCGAG
PmaCI 2867 CACGTG CAC^GTG
PmlI 2867 CACGTG CAC^GTG
RsrII 1524 CGGACCG CG^G[AT]CCG
SacI 1090 GAGCTC GAGCT^C
SalI 2051 GTCGAC G^TCGAC
SbfI 3755 CCTGCAGG CCTGCA^GG
SmaI 1915 CCCGGG CCC^GGG
SpeI 2370 ACTAGT A^CTAGT
Sse8387I 3755 CCTGCAGG CCTGCA^GG
SspBI 896 TGTACA T^GTACA
SspI 509 AATATT AAT^ATT
XhoI 758 CTCGAG C^TCGAG
XmaCI 1915 CCCGGG C^CCGGG
XmaI 1915 CCCGGG C^CCGGG
XmnI 804 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 2024 GAATTT [GA]^AATT[CT]
2999 AAATTT
ApoI 2024 GAATTT [GA]^AATT[CT]
2999 AAATTT
BbvCI 3617 CCTCAGC CCTCAGC(-5/-2)
3749 CCTCAGC
BglII 752 AGATCT A^GATCT
956 AGATCT
BsgI 520 GTGCAG GTGCAG(16/14)
1620 GTGCAG
BsmI 3284 GCATTC GAATGC(1/-1)
3560 GCATTC
BspLU11I 1762 ACATGT A^CATGT
4269 ACATGT
BsrBI 3172 CCGCTC CCGCTC(-3/-3)
1551 GAGCGG
BssSI 1739 CACGAG CACGAG(-5/-1)
4069 CACGAG
BstEII 915 GGTGACC G^GT[ACGT]ACC
3062 GGTAACC
BstPI 915 GGTGACC G^GT[ACGT]ACC
3062 GGTAACC
DraIII 123 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3924 CACACTGTG
EcoO65I 915 GGTGACC G^GT[ACGT]ACC
3062 GGTAACC
HindIII 136 AAGCTT A^AGCTT
3596 AAGCTT
MfeI 1103 CAATTG C^AATTG
1405 CAATTG
MunI 1103 CAATTG C^AATTG
1405 CAATTG
SfiI 2842 GGCCATCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3070 GGCCCCTCTGGCC
SphI 2423 GCATGC GCATG^C
3116 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DrdI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII